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BMC Oral Health

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Open Access 01-12-2023 | Research

Evaluation of different decontamination procedures on bond strength to sound and caries affected dentin using “no-wait” universal adhesive

Authors: Lamiaa M Moharam, Haidy N Salem, Sherif Khadr, Ahmed Abdou

Published in: BMC Oral Health | Issue 1/2023

Abstract

Background

Current study aimed to evaluate the effect of different decontamination procedures on micro-shear bond strength (μSBS) of sound (SoD) and caries-affected dentin (CAD) of two universal adhesives after blood-saliva contamination.

Methods

One hundred and eighty bovine anterior teeth were prepared and allocated into the respective groups according to tested dentin substrates [SoD, CAD], universal adhesives [Clearfil Bond Universal Quick (UBQ), All-Bond-Universal (ABU)], adhesive contamination stage [none, contamination before and after adhesives light-curing], and according to decontamination procedures [no decontamination, water rinsing, adhesive rebond, Ethylenediaminetetraacetic acid (ETDA) and chlorhexidine (CHX) application]. Universal adhesives were applied according to manufacturer instructions in self-etch (SE) bonding mode. Four composite microrods were built for each tooth. Specimens were kept in distilled water for 24 hours at 37°C before testing μSBS. Four-way ANOVA and Tukey HSD tests were used for data analysis.

Results

A statistically significant difference between contamination stages of both universal adhesives at different decontamination procedures for SoD and CAD. Highest μSBS was recorded for UBQ control group at SoD, while the least was recorded for light-cured ABU upon water rinsing decontamination procedure of CAD.

Conclusions

Proper cavity isolation is mandatory to avoid possible contamination which can dramatically affect μSBS. CHX is a potent cavity decontaminant that can restore different dentin substrates bond strength. EDTA presents a promising substitute. UBQ adhesive showed better bonding performance than ABU to both dentin substrates. Application of regular cavity decontamination approaches is highly advised in daily practice to avoid possible detrimental effect of accidental cavity contamination.

Ahmed MH, Yoshihara K, Mercelis B, Van Landuyt K, Peumans M, Van Meerbeek B. Quick bonding using a universal adhesive. Clin Oral Investig. 2020;24:2837–51.PubMed

Moharam LM, Afifi RH. Influence of adhesive application method and thermocycling on the bonding performance of different adhesive systems to dentin. J Int Oral Health. 2023;15:168.

Perdigão J. Current perspectives on dental adhesion: (1) dentin adhesion – not there yet. Jpn Dent Sci Rev. 2020;56:190–207.PubMedPubMedCentral

Llerena-Icochea AE, Costa RM, Borges A, Bombonatti J, Furuse AY. Bonding polycrystalline zirconia with 10-MDP-containing adhesives. Oper Dent. 2017;42:335–41.PubMed

Papadogiannis D, Dimitriadi M, Zafiropoulou M, Gaintantzopoulou MD, Eliades D. Universal adhesives: setting characteristics and reactivity with dentin. Mater (Basel). 2019;12:1720.

Kalay TS, Zaim B. Effect of alternative self-etch applications on dentin bond strength of “no wait concept” universal adhesives. ODOVTOS-Int J Dent Sci. 2022;24:58–66.

Saikaew P, Chowdhury AF, Fukuyama M, Kakuda S, Carvalho RM, Sano H. The effect of dentine surface preparation and reduced application time of adhesive on bonding strength. J Dent. 2016;47:63–70.PubMed

Huang XQ, Pucci CR, Luo T, Breschi L, Pashley DH, Niu LN, Tay FR. No- waiting dentine self-etching concept-merit or hype. J Dent. 2017;62:54–63.PubMed

Sofan E, Sofan A, Palaia G, Tenore G, Romeo U, Migliau G. Classification review of dental adhesive systems: from the IV generation to the universal type. Ann Stomatol (Roma). 2017;8:1–17.PubMedPubMedCentral

10.

Isolan CP, Sarkis-Onofre R, Giana S, Lima GS, Moraes RR. Bonding to sound and caries-affected dentin: a systematic review and meta-analysis. J Adhes Dent. 2018;20:7–18.PubMed

11.

Shibata S, Vieira LC, Baratieri LN, Fu J, Hoshika S, Matsuda Y, et al. Evaluation of microtensile bond strength of self-etching adhesives on normal and caries-affected dentin. Dent Mater J. 2016;35:166–73.PubMed

12.

Kusumasari C, Abdou A, Nakajima M, Tagami J. Deproteinization of caries-affected dentin with chemo-mechanical caries removal agents and its effect on dentin bonding with self-etch adhesives. J Dent. 2021;109:103665.PubMed

13.

Prasad M, Mohamed S, Nayak K, Shetty SK, Talapaneni AK. Effect of moisture, saliva, and blood contamination on the shear bond strength of brackets bonded with a conventional bonding system and self–etched bonding system. J Nat Sci Biol Med. 2014;5:123–9.PubMedPubMedCentral

14.

Haralur SB, Alharthi SM, Abohasel SA, Alqahtani KM. Effect of decontamination treatments on micro-shear bond strength between blood–saliva-contaminated post-etched dentin substrate and composite resin. Healthcare. 2019;7:128.PubMedPubMedCentral

15.

Mempel CA, Jacker-Guhr S, Lührs AK. Contamination of dentin with hemostatic agents - is EDTA a valuable decontaminant before using a self-etch universal adhesive? J Adhes Dent. 2022;24:345–54.PubMed

16.

Coelho A, Amaro I, Rascão B, Marcelino I, Paula A, Saraiva J, et al. Effect of cavity disinfectants on dentin bond strength and clinical success of composite restorations: a systematic review of in vitro, in situ and clinical studies. Int J Mol Sci. 2021;22:353.

17.

Moharam LM, Salem HN, Elgamily HM. The effect of incorporating different concentrations of chlorhexidine digluconate on the degree of conversion of an experimental adhesive resin. J Clin Exp Dent. 2018;10:e371–7.PubMedPubMedCentral

18.

Marchesi G, Frassetto A, Mazzoni A, Apolonio F, Diolosa M, Cadenaro M, et al. Adhesive performance of a multi-mode adhesive system: 1-year in vitro study. J Dent. 2014;42:603–12.PubMed

19.

Coelho A, Vilhena L, Antunes M, Amaro I, Paula A, Marto CM, Saraiva J, et al. Effect of different cavity disinfectants on adhesion to dentin of permanent teeth. J Funct Biomater. 2022;13:209.PubMedPubMedCentral

20.

Kasraei S, Yarmohamadi E, Ranjbaran Jahromi P, Akbarzadeh M. Effect of 940nm diode laser irradiation on microtensile bond strength of an etch and rinse adhesive (single bond 2) to dentin. J Dent (Shiraz). 2019;20:30–6.PubMed

21.

Atef Y, Yousry T, El Harouny NM. Shear bond strength of metallic brackets bonded to enamel pretreated with Er,Cr: YSGG LASER and CPP-ACP. BMC Oral Health. 2021;21:1.

22.

Bayraktar G, Guvener B, Bural C, Uresin Y. Influence of polymerization method, curing process, and length of time of storage in water on the residual methyl methacrylate content in dental acrylic resins. J Biomed Mater Res B: Appl Biomater. 2006;76:340–5.PubMed

23.

Elkassas D, Arafa A. Assessment of post contamination treatments affecting different bonding stages to dentin. Eur J Dent. 2016;10:327–32.PubMedPubMedCentral

24.

Nicoloso GF, Antoniazzi BF, Lenzi TL, Soares FZM, Rocha RO. The bonding performance of a universal adhesive to artificially created caries-affected dentin. J Adhes Dent. 2017;19:317–21.PubMed

25.

Bolme J, Gjerdet R, Torgils Lægreid R. Effect of saliva contamination on the bond strength of single-step and three-step adhesive systems. Eur J Oral Sci. 2022;130:e12838.PubMed

26.

Mohanty PR, Mishra L, Saczuk K, Lapinska B. Optimizing Adhesive Bonding to Caries affected dentin: a comprehensive systematic review and Meta-analysis of Dental Adhesive Strategies following chemo-mechanical caries removal. Appl Sci. 2023;13:7295.

27.

Kusumasari C, Abdou A, Tichy A, Hatayama T, Hosaka K, Foxton RM, et al. Effect of smear layer deproteinization with chemo-mechanical caries removal agents on sealing performances of self-etch adhesives. J Dent. 2020;94:103300.PubMed

28.

Lund RG, Da Silveira IA, Ribeiro JS, Rubin D, Peralta SL, Cuevas–Suárez CE, et al. Influence of blood contamination and decontamination procedures on bond strength of a two–step etch and rinse adhesive system. Eur J Gen Dent. 2019;8:71–5.

29.

Nair P, Ilie N. The long-term consequence of salivary contamination at various stages of adhesive application and clinically feasible remedies to decontaminate. Clin Oral Investig. 2020;24:4413–26.PubMedPubMedCentral

30.

Oonsombat C, Bishara SE, Ajlouni R. The effect of blood contamination on the shear bond strength of orthodontic brackets with the use of a new self-etch primer. Am J Orthod Dentofac Orthop. 2003;123:547–50.

31.

Irmak Ö, Yaman BC, Orhan EO, Ozer F, Blatz MB. Effect of rubbing force magnitude on bond strength of universal adhesives applied in self-etch mode. Dent Mater J. 2018;37:139–45.PubMed

32.

Yazici AR, Tuncer D, Dayangaç B, Ozgünaltay G, Onen A. The effect of saliva contamination on microleakage of an etch-and-rinse and a self-etching adhesive. J Adhes Dent. 2007;9:305–9.PubMed

33.

de Carvalho MEC, Vieira SN, Kawaguchi FA, Powers J, Matos AB. Influence of blood contamination on bond strength of a self-etching system. Eur J Dent. 2010;4:280–6.

34.

Chang SW, Cho BH, Lim RY, Kyung SH, Park DS, Oh TS, et al. Effects of blood contamination on microtensile bond strength to dentin of three self-etch adhesives. Oper Dent. 2010;35:330–6.PubMed

35.

Pashley DH, Tay FR, Yiu C, Hashimoto M, Breschi L, Carvalho RM. Collagen degradation by host-derived enzymes during aging. J Dent Res. 2004;83:216–21.PubMed

36.

Ebrahimi M, Majidinia S, Sarraf A. Effect of chlorhexidine on immediate and delayed bond strength between resin and dentin of primary teeth: a systematic review and meta-analysis. Front Dent. 2022;31:19–39.

37.

Elkassas DW, Fawzi EM, El Zohairy A. The effect of cavity disinfectants on the micro-shear bond strength of dentin adhesives. Eur J Dent. 2014;8:184–90.PubMedPubMedCentral

38.

Meiers JC, Kresin JC. Cavity disinfectants and dentin bonding. Oper Dent. 1996;21:153–9.PubMed

39.

Furuse AY, da Cunha LF, Benetti AR, Mondelli J. Bond strength of resin-resin interfaces contaminated with saliva and submitted to different surface treatments. J Appl Oral Sci. 2007;15:6.

40.

Bakry AS, Abbassy MA. Application modes affect two universal adhesive systems’ nanoleakage expression and shear bond strength. BioMed Res Int. 2021; 7375779.

41.

De Munck J, Poitevin A, Lührs AK, Pongprueksa P, Van Ende A, Van Lan duyt KL, Van Meerbeek B. Interfacial fracture toughness of aged adhesive-dentin interfaces. Dent Mater. 2015;31:462–72.PubMed

42.

Yoshihara K, Nagaoka N, Nakamura A, Hara T, Yoshida Y, Van Meerbeek B. Nano-layering adds strength to the adhesive interface. J Dent Res. 2020;100:515–21.PubMed

Title: Evaluation of different decontamination procedures on bond strength to sound and caries affected dentin using “no-wait” universal adhesive
Authors: Lamiaa M Moharam
Haidy N Salem
Sherif Khadr
Ahmed Abdou
Publication date: 01-12-2023
Publisher: BioMed Central
Published in: BMC Oral Health / Issue 1/2023
Electronic ISSN: 1472-6831
DOI: https://doi.org/10.1186/s12903-023-03314-2

2024 ASCO Annual Meeting

Springer Medicine

Evaluation of different decontamination procedures on bond strength to sound and caries affected dentin using “no-wait” universal adhesive

Abstract

Background

Methods

Results

Conclusions

2024 ASCO Annual Meeting

Springer Medicine

Abstract

Background

Methods

Results

Conclusions

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