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01-03-2018 | Original Article

Evaluation of combination therapy with hydrocortisone, vitamin C, and vitamin E in a rat model of intestine ischemia-reperfusion injury

Authors: Mehdi Tavasoli, Omid Azari, Reza Kheirandish, Mohammad Farajli Abbasi

Published in: Comparative Clinical Pathology | Issue 2/2018

Abstract

This study was designed to use different antioxidant as hydrocortisone, vitamin C, vitamin E, and combination of them against experimental intestine ischemia-reperfusion (IR) for decrease free radical damage in soft tissue. Thirty adult male Wistar rats were selected randomly and divided into six equal groups. Group Sham: intact group, group control: IR group with 45 min of ischemia followed by 1 h of reperfusion, group vitamin C: IR plus vitamin C (50 mg/kg, IV) treatment immediately after reperfusion, group vitamin E: IR plus vitamin E (20 mg/kg, IM) treatment 15 min before reperfusion, group hydrocortisone: IR plus hydrocortisone (50 mg/kg, IV) treatment immediately after reperfusion and group combination: IR plus combination of vitamin C, E, and hydrocortisone. Ischemia was induced by clamping the supraciliac artery. In the end of time of reperfusion, the proximal segment of jejunum was collected as a tissue specimen for histopathologic examination. In histopathological study, severe damage was detected in intestinal mucosa to all control and treatment groups, and histopathologic score was significantly decreased in the treatment groups. Analysis of the histopathological results showed that in the all treatment groups, the intestinal mucosal injury was decreased compared to the control group and in the combination group, the protection was better than the other treatment groups. The results showed simultaneous administration of vitamin C, E, and hydrocortisone combination before reperfusion of blood flow to the ischemic tissue could improve the deleterious effects of IR injuries in intestinal mucosa.

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Title: Evaluation of combination therapy with hydrocortisone, vitamin C, and vitamin E in a rat model of intestine ischemia-reperfusion injury
Authors: Mehdi Tavasoli
Omid Azari
Reza Kheirandish
Mohammad Farajli Abbasi
Publication date: 01-03-2018
Publisher: Springer London
Published in: Comparative Clinical Pathology / Issue 2/2018
Print ISSN: 1618-5641
Electronic ISSN: 1618-565X
DOI: https://doi.org/10.1007/s00580-017-2610-4

At a glance: The STEP trials

Springer Medicine

Evaluation of combination therapy with hydrocortisone, vitamin C, and vitamin E in a rat model of intestine ischemia-reperfusion injury

Abstract

At a glance: The STEP trials

Springer Medicine

Abstract

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