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Archives of Virology
Published in: Archives of Virology 5/2019

01-05-2019 | Brief Report

Establishment of a rescue system for porcine parvovirus using a seamless cloning method

Authors: Lingling Zhang, Dou Gao, Yongle Yu, Yebing Liu, Weiquan Liu, Jinxiang Li, Shangjin Cui

Published in: Archives of Virology | Issue 5/2019

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Abstract

In this study, we describe a novel and rapid method for the construction of a full-length infectious clone (pPPV). The constructed clone contained an engineered EcoRv site that served as a genetic marker and was shown to be infectious when transfected into a monolayer of PK-15 cells. The rescued virus (rPPV) of the infectious clone was found to be indistinguishable from wild-type virus BQ in terms of its biological properties. The generation of this PPV infectious clone provides a potentially powerful tool with which to elucidate the molecular pathogenesis of PPV.
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Metadata
Title
Establishment of a rescue system for porcine parvovirus using a seamless cloning method
Authors
Lingling Zhang
Dou Gao
Yongle Yu
Yebing Liu
Weiquan Liu
Jinxiang Li
Shangjin Cui
Publication date
01-05-2019
Publisher
Springer Vienna
Published in
Archives of Virology / Issue 5/2019
Print ISSN: 0304-8608
Electronic ISSN: 1432-8798
DOI
https://doi.org/10.1007/s00705-019-04209-w

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