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01-06-2018 | Original Article

ER stress and genomic instability induced by gamma radiation in mice primary cultured glial cells

Authors: Jit Chatterjee, Rajesha K. Nairy, Jaldeep Langhnoja, Ashutosh Tripathi, Rajashekhar K. Patil, Prakash P. Pillai, Mohammed S. Mustak

Published in: Metabolic Brain Disease | Issue 3/2018

Abstract

Ionizing radiation induces various pathophysiological conditions by altering central nervous system (CNS) homeostasis, leading to neurodegenerative diseases. However, the potential effect of ionizing radiation response on cellular physiology in glial cells is unclear. In the present study, micronucleus test, comet assay, and RT-PCR were performed to investigate the potential effect of gamma radiation in cultured oligodendrocytes and astrocytes with respect to genomic instability, Endoplasmic Reticulum (ER) stress, and inflammation. Further, we studied the effect of alteration in ER stress specific gene expression in cortex post whole body radiation in mice. Results showed that exposure of gamma radiation of 2Gy in-vitro cultured astrocytes and oligodendrocytes and 7Gy in-vivo induced ER stress and Inflammation along with profuse DNA damage and Chromosomal abnormality. Additionally, we observed downregulation of myelin basic protein levels in cultured oligodendrocytes exposed to radiation. The present data suggests that ER stress and pro inflammatory cytokines serve as the major players in inducing glial cell dysfunction post gamma irradiation along with induction of genomic instability. Taken together, these results indicate that ER stress, DNA damage, and inflammatory pathways may be critical events leading to glial cell dysfunction and subsequent cell death following exposure to ionizing radiation.

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Title: ER stress and genomic instability induced by gamma radiation in mice primary cultured glial cells
Authors: Jit Chatterjee
Rajesha K. Nairy
Jaldeep Langhnoja
Ashutosh Tripathi
Rajashekhar K. Patil
Prakash P. Pillai
Mohammed S. Mustak
Publication date: 01-06-2018
Publisher: Springer US
Published in: Metabolic Brain Disease / Issue 3/2018
Print ISSN: 0885-7490
Electronic ISSN: 1573-7365
DOI: https://doi.org/10.1007/s11011-018-0183-9

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ER stress and genomic instability induced by gamma radiation in mice primary cultured glial cells

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