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BMC Complementary Medicine and Therapies
Published in: BMC Complementary Medicine and Therapies 1/2023

Open Access 01-12-2023 | Enteropathogenic Escherichia Coli | Research

Encapsulation of Polygonum bistorta root phenolic compounds as a novel phytobiotic and its protective effects in the mouse model of enteropathogenic Escherichia coli infection

Authors: Zahra kadkhoda Mezerji, Reza Boshrouyeh, Seyedehfarnaz Hafezian Razavi, Shaghayegh Ghajari, Hasti Hajiha, Negin Shafaei, Ehsan Karimi, Ehsan Oskoueian

Published in: BMC Complementary Medicine and Therapies | Issue 1/2023

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Abstract

Background

Microencapsulation technology is the fundamental delivery system for encapsulating the natural bioactive compounds especially phenolic in order to developing bioavailability, stability and controlling release. This study was conducted to determine the antibacterial and health-promoting potential of the phenolic rich extract (PRE)-loaded microcapsules obtained from Polygonum bistorta root as a dietary phytobiotic in mice challenged by enteropathogenic Escherichia coli (E. coli).

Method

The PRE was obtained from Polygonum bistorta root using fractionation by different polarity solvents and the highest PRE was encapsulated by the combination of modified starch, maltodextrin, and whey protein concentrate as wall materials using a spray dryer. Then, the physicochemical characterization (particle size, zeta potential, Morphology and polydispersity index) of microcapsules have been assessed. For the invivo study, 30 mice at five treatment were designed and antibacterial properties were analyzed. Furthermore, relative fold changes in the ileum population of E. coli was investigated using Real time PCR.

Results

The encapsulation of PRE resulted in the production of phenolic enriched extract-loaded microcapsules (PRE-LM) with a mean diameter of 330 nm and relatively high entrapment efficiency (87.2% w/v). The dietary supplementation of PRE-LM improved weight gain, liver enzymes, gene expression, morphometric characteristics of the ileum and decreased the population of E. coli present in the ileum significantly (p < 0.05).

Conclusion

Our funding suggested PRE-LM as a promising phytobiotic against E. coli infection in mice.
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Metadata
Title
Encapsulation of Polygonum bistorta root phenolic compounds as a novel phytobiotic and its protective effects in the mouse model of enteropathogenic Escherichia coli infection
Authors
Zahra kadkhoda Mezerji
Reza Boshrouyeh
Seyedehfarnaz Hafezian Razavi
Shaghayegh Ghajari
Hasti Hajiha
Negin Shafaei
Ehsan Karimi
Ehsan Oskoueian
Publication date
01-12-2023
Publisher
BioMed Central
Published in
BMC Complementary Medicine and Therapies / Issue 1/2023
Electronic ISSN: 2662-7671
DOI
https://doi.org/10.1186/s12906-023-03868-2

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