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Journal of Natural Medicines
Published in: Journal of Natural Medicines 2/2016

01-04-2016 | Original Paper

Enhancement of energy production by black ginger extract containing polymethoxy flavonoids in myocytes through improving glucose, lactic acid and lipid metabolism

Authors: Kazuya Toda, Shogo Takeda, Shoketsu Hitoe, Seikou Nakamura, Hisashi Matsuda, Hiroshi Shimoda

Published in: Journal of Natural Medicines | Issue 2/2016

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Abstract

Enhancement of muscular energy production is thought to improve locomotive functions and prevent metabolic syndromes including diabetes and lipidemia. Black ginger (Kaempferia parviflora) has been cultivated for traditional medicine in Thailand. Recent studies have shown that black ginger extract (KPE) activated brown adipocytes and lipolysis in white adipose tissue, which may cure obesity-related dysfunction of lipid metabolism. However, the effect of KPE on glucose and lipid utilization in muscle cells has not been examined yet. Hence, we evaluated the effect of KPE and its constituents on energy metabolism in pre-differentiated (p) and differentiated (d) C2C12 myoblasts. KPE (0.1–10 μg/ml) was added to pC2C12 cells in the differentiation process for a week or used to treat dC2C12 cells for 24 h. After culturing, parameters of glucose and lipid metabolism and mitochondrial biogenesis were assessed. In terms of the results, KPE enhanced the uptake of 2-deoxyglucose and lactic acid as well as the mRNA expression of glucose transporter (GLUT) 4 and monocarboxylate transporter (MCT) 1 in both types of cells. The expression of peroxisome proliferator-activated receptor γ coactivator (PGC)-1α was enhanced in pC2C12 cells. In addition, KPE enhanced the production of ATP and mitochondrial biogenesis. Polymethoxy flavonoids in KPE including 5-hydroxy-7-methoxyflavone, 5-hydroxy-3,7,4′-trimethoxyflavone and 5,7-dimethoxyflavone enhanced the expression of GLUT4 and PGC-1α. Moreover, KPE and 5,7-dimethoxyflavone enhanced the phosphorylation of 5′AMP-activated protein kinase (AMPK). In conclusion, KPE and its polymethoxy flavonoids were found to enhance energy metabolism in myocytes. KPE may improve the dysfunction of muscle metabolism that leads to metabolic syndrome and locomotive dysfunction.
Literature
1.
2.
3.
Rooyackers OE, Adey DB, Ades PA, Nair KS (1996) Effect of age on in vivo rates of mitochondrial protein synthesis in human skeletal muscle. Proc Natl Acad Sci USA 93:15364–15369PubMedCentralCrossRefPubMed
4.
Barazzoni R, Short KR, Nair KS (2000) Effects of aging on mitochondrial DNA copy number and cytochrome c oxidase gene expression in rat skeletal muscle, liver, and heart. J Biol Chem 275:3343–3347CrossRefPubMed
5.
Dirks AJ, Hofer T, Marzetti E, Pahor M, Leeuwenburgh C (2006) Mitochondrial DNA mutations, energy metabolism and apoptosis in aging muscle. Ageing Res Rev 5:179–195CrossRefPubMed
6.
7.
Petersen KF, Befroy D, Dufour S, Dziura J, Ariyan C, Rothman DL, DiPietro L, Cline GW, Shulman GI (2003) Mitochondrial dysfunction in the elderly: possible role in insulin resistance. Science 300:1140–1142PubMedCentralCrossRefPubMed
8.
Montessuit C, Rosenblatt-Velin N, Papageorgiou I, Campos L, Pellieux C, Palma T, Lerch R (2004) Regulation of glucose transporter expression in cardiac myocytes: p38 MAPK is a strong inducer of GLUT4. Cardiovasc Res 64:94–104CrossRefPubMed
9.
Richter EA, Hargreaves M (2013) Exercise, GLUT4, and skeletal muscle glucose uptake. Physiol Rev 93:993–1017CrossRefPubMed
10.
11.
12.
dos Santos JM, Benite-Ribeiro SA, Queiroz G, Duarte JA (2012) The effect of age on glucose uptake and GLUT1 and GLUT4 expression in rat skeletal muscle. Cell Biochem Funct 30:191–197CrossRefPubMed
13.
Akase T, Shimada T, Terabayashi S, Ikeya Y, Sanada H, Aburada M (2011) Antiobesity effects of Kaempferia parviflora in spontaneously obese type II diabetic mice. J Nat Med 65:73–80CrossRefPubMed
14.
Shimada T, Horikawa T, Ikeya Y, Matsuo H, Kinoshita K, Taguchi T, Ichinose K, Takahashi K, Aburada M (2011) Preventive effect of Kaempferia parviflora ethyl acetate extract and its major components polymethoxyflavonoid on metabolic diseases. Fitoterapia 82:1272–1278CrossRefPubMed
15.
Rujjanawate C, Kanjanapothi D, Amornlerdpison D, Pojanagaroon SJ (2005) Anti-gastric ulcer effect of Kaempferia parviflora. Ethno pharmacol 102:120–122
16.
Kusirisin W, Srichairatanakool S, Lerttrakarnnon P, Lailerd N, Suttajit M, Jaikang C, Chaiyasut C (2009) Antioxidative activity, polyphenolic content and anti-glycation effect of some Thai medicinal plants traditionally used in diabetic patients. Med Chem 5:139–147CrossRefPubMed
17.
Chaturapanich G, Chaiyakul S, Verawatnapakul V, Yimlamai T, Pholpramool C (2012) Enhancement of aphrodisiac activity in male rats by ethanol extract of Kaempferia parviflora and exercise training. Andrologia 44:323–328CrossRefPubMed
18.
Chaipech S, Morikawa T, Ninomiya K, Yoshikawa M, Pongpiriyadacha Y, Hayakawa T, Muraoka O (2012) Structures of two new phenolic glycosides, kaempferiaosides A and B, and hepatoprotective constituents from the rhizomes of Kaempferia parviflora. Chem Pharm Bull 60:62–69CrossRefPubMed
19.
Sutthanut K, Sripanidkulchai B, Yenjai C, Jay M (2007) Simultaneous identification and quantitation of 11 flavonoid constituents in Kaempferia parviflora by gas chromatography. J Chromatography A 1143:227–233CrossRef
20.
Murata K, Hayashi H, Matsumura S, Matsuda H (2013) Suppression of benign prostate hyperplasia by Kaempferia parviflora rhizome. Pharmacognosy Res 5:309–314PubMedCentralCrossRefPubMed
21.
Yenjai C, Wanich S, Pitchuanchom S, Sripanidkulchai B (2009) Structural modification of 5,7-dimethoxyflavone from Kaempferia parviflora and biological activities. Arch Pharm Res 32:1179–1184CrossRefPubMed
22.
Patanasethanont D, Nagai J, Matsuura C, Fukui K, Sutthanut K, Sripanidkulchai BO, Yumoto R, Takano M (2007) Modulation of function of multidrug resistance associated-proteins by Kaempferia parviflora extracts and their components. Eur J Pharmacol 566:67–74CrossRefPubMed
23.
Sae-Wong C, Matsuda H, Tewtrakul S, Tansakul P, Nakamura S, Nomura Y, Yoshikawa M (2011) Suppressive effects of methoxyflavonoids isolated from Kaempferia parviflora on inducible nitric oxide synthase (iNOS) expression in RAW 264.7 cells. J Ethnopharm 136:488–495CrossRef
24.
Lee YS, Cha BY, Saito K, Yamakawa H, Choi SS, Yamaguchi K, Yonezawa T, Teruya T, Nagai K, Woo JT (2010) Nobiletin improves hyperglycemia and insulin resistance in obese diabetic ob/ob mice. Biochem Pharmacol 79:1674–1683CrossRefPubMed
25.
Matsushita M, Yoneshiro T, Aita S, Kamiya T, Kusaba N, Yamaguchi K, Takagaki K, Kameya T, Sugie H, Saito M (2015) Kaempferia parviflora extract increases whole-body energy expenditure in humans. Roles of brown adipose tissue. J Nutr Sci Vitaminol 61:79–83 (Tokyo) CrossRefPubMed
26.
Promthep K, Eungpinichpong W, Sripanidkulchai B, Chatchawan U (2015) Effect of Kaempferia parviflora extract on physical fitness of soccer players. A randomized double-blind placebo-controlled trial. Med Sci Monit Basic Res 21:100–108PubMedCentralPubMed
27.
Yoshino S, Kim M, Awa R, Kuwahara H, Kano Y, Kawada T (2014) Kaempferia parviflora extract increases energy consumption through activation of BAT in mice. Food Sci Nutr 2:634–637PubMedCentralCrossRefPubMed
28.
Okabe Y, Shimada T, Horikawa T, Kinoshita K, Koyama K, Ichinose K, Aburada M, Takahashi K (2014) Suppression of adipocyte hypertrophy by polymethoxyflavonoids isolated from Kaempferia parviflora. Phytomedicine 21:800–806CrossRefPubMed
29.
Montessuit C, Rosenblatt-Velin N, Papageorgiou I, Campos L, Pellieux C, Palma T, Lerch R (2004) Regulation of glucose transporter expression in cardiac myocytes: p38 MAPK is a strong inducer of GLUT4. Cardiovasc Res 64:94–104CrossRefPubMed
30.
31.
Handschin C, Spiegelman BM (2011) PGC-1 coactivators and the regulation of skeletal muscle fiber-type determination. Cell Metabol 13:351CrossRef
32.
Lira VA, Brown DL, Lira AK, Kavazis AN, Soltow QA, Zeanah EH, Criswell DS (2010) Nitric oxide and AMPK cooperatively regulate PGC-1 in skeletal muscle cells. J Physiol 588(Pt 18):3551–3566PubMedCentralCrossRefPubMed
33.
34.
Kim MS, Hur HJ, Kwon DY, Hwang JT (2012) Tangeretin stimulates glucose uptake via regulation of AMPK signaling pathways in C2C12 myotubes and improves glucose tolerance in high-fat diet-induced obese mice. Mol Cell Endocrinol 358:127–134CrossRefPubMed
35.
Tsutsumi R, Yoshida T, Nii Y, Okahisa N, Iwata S, Tsukayama M, Hashimoto R, Taniguchi Y, Sakaue H, Hosaka T, Shuto E, Sakai T (2014) Sudachitin, a polymethoxylated flavone, improves glucose and lipid metabolism by increasing mitochondrial biogenesis in skeletal muscle. Nutr Metab (Lond) 4(11):32. doi:10.​1186/​1743-7075-11-32 CrossRef
36.
Matsuda H, Kogami Y, Nakamura S, Sugiyama T, Ueno T, Yoshikawa M (2011) Structural requirements of flavonoids for the adipogenesis of 3T3-L1 cells. Bioorg Med Chem 19:2835–2841CrossRefPubMed
Metadata
Title
Enhancement of energy production by black ginger extract containing polymethoxy flavonoids in myocytes through improving glucose, lactic acid and lipid metabolism
Authors
Kazuya Toda
Shogo Takeda
Shoketsu Hitoe
Seikou Nakamura
Hisashi Matsuda
Hiroshi Shimoda
Publication date
01-04-2016
Publisher
Springer Japan
Published in
Journal of Natural Medicines / Issue 2/2016
Print ISSN: 1340-3443
Electronic ISSN: 1861-0293
DOI
https://doi.org/10.1007/s11418-015-0948-y

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