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Neuroradiology

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01-10-2009 | Interventional Neuroradiology

Endovascular transplantation of stem cells to the injured rat CNS

Authors: Johan Lundberg, Katarina Le Blanc, Mikael Söderman, Tommy Andersson, Staffan Holmin

Published in: Neuroradiology | Issue 10/2009

Abstract

Introduction

Transplantation procedures using intraparenchymal injection of stem cells result in tissue injury in addition to associated surgical risks. Intravenous injection of mesenchymal stem cells gives engraftment to lesions, but the method has low efficiency and specificity. In traumatic brain injuries (TBI), there is a transient breakdown of the blood–brain barrier and an inflammatory response, which increase migration of cells from blood to parenchyma. The aim of this investigation was to analyze the effect of intra-arterial administration on cellular engraftment.

Methods

Experimental TBI was produced in a rat model. Endovascular technique was used to administer human mesenchymal stem cells in the ipsilateral internal carotid artery. Evaluation of engraftment and side effects were performed by immunohistochemical analysis of the brain and several other organs. The results were compared to intravenous administration of stem cells.

Results

Intra-arterial transplantion of mesenchymal stem cells resulted in central nervous system (CNS) engraftment without thromboembolic ischemia. We observed a significantly higher number of transplanted cells in the injured hemisphere after intra-arterial compared to intravenous administration both 1 day (p < 0.01) and 5 days (p < 0.05) after the transplantation. Some cells were also detected in the spleen but not in the other organs analyzed.

Conclusion

Selective intra-arterial administration of mesenchymal stem cells to the injured CNS is a minimally invasive method for transplantation. The method is significantly more efficient than the intravenous route and causes no side effects in the current model. The technique can potentially be used for repeated transplantation to the CNS after TBI and in other diseases.

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Title: Endovascular transplantation of stem cells to the injured rat CNS
Authors: Johan Lundberg
Katarina Le Blanc
Mikael Söderman
Tommy Andersson
Staffan Holmin
Publication date: 01-10-2009
Publisher: Springer-Verlag
Published in: Neuroradiology / Issue 10/2009
Print ISSN: 0028-3940
Electronic ISSN: 1432-1920
DOI: https://doi.org/10.1007/s00234-009-0551-6

Keynote webinar | Spotlight on medication adherence

Springer Medicine

Endovascular transplantation of stem cells to the injured rat CNS

Abstract

Introduction

Methods

Results

Conclusion

Keynote webinar | Spotlight on medication adherence

Springer Medicine

Abstract

Introduction

Methods

Results

Conclusion

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