Published in:
01-01-2018 | Original Article
Emergence of multi drug resistance strains causing diabetic foot infection in Salem, Tamil Nadu, India
Authors:
Pugazhendhi Sugandhi, Dorairaj Arvind Prasanth
Published in:
International Journal of Diabetes in Developing Countries
|
Issue 1/2018
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Abstract
This study was to assess the emergence of mecA and bla
CTX-M
in multidrug resistant MRSA/MRSE and ESBL strains in diabetic foot infections. A total of 160 pus samples were collected from the diabetic foot care hospitals, Salem, Tamil Nadu, India. The samples were processed for microbiological investigations and further evaluating the resistant strains. The bacterial strain Staphylococcus aureus was the predominant organisms isolated from the sample. Cefoxitin, Oxacillin and Vancomycin were found to be the most effective antimicrobial agents for therapy of gram positive organisms while Meropenem, Piperacillin, Cefoperazone/Sulbactam, Piperacillin/ Tazobactam and Amikacin were found to be the most effective antimicrobial agents for the gram negative organisms. In this study, four isolates of Methicillin resistant Staphylococcus aureus (MRSA) strains, one isolate of Methicillin resistant Staphylococcus epidermidis (MRSE) and five isolates of Pseudomonas aeruginosa were found to be extended spectrum of β - lactamase (ESBL) producers by phenotypic method. These strains subjected to genotypic confirmation confirmed the presence of resistant genes namely bla
CTX-M
in ESBL and SCC mecA in Staphylococcus spp. The 16S rRNA partial gene sequencing and phylogenetic analysis of the multi drug resistant organisms Pseudomonas aeruginosa (APS01) and Staphylococcus epidermidis (APS02) was confirmed the resistant pattern in these strains. The resistant organism was eradicated only by the effective management of this infection; knowledge on the causative agent causing cruel diabetic foot infections will be very useful in selecting the appropriate therapy.