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Tumor Biology
Published in: Tumor Biology 12/2015

01-12-2015 | Research Article

Elevated expression of TGIF is involved in lung carcinogenesis

Authors: Yadong Wang, Haiyu Wang, Huiyan Gao, Bing Xu, Wenlong Zhai, Jiangmin Li, Congke Zhang

Published in: Tumor Biology | Issue 12/2015

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Abstract

The purpose of this study was to explore the expression of TG-interacting factor (TGIF) in lung carcinogenesis. Malignant transformation of human bronchial epithelial (16HBE) cell was established by benzo(a)pyrene (BaP) treatment. Soft agar assay and tumor formation assay in nude mice were applied. Tumorigenesis experiment in vivo was done by BaP treatment. Western blotting, immunohistochemistry, and quantitative polymerase chain reaction were used to detect TGIF expression. We observed a higher level of TGIF messenger RNA (mRNA) in lung cancer tissues than that in paracancerous tissues. We observed significantly higher levels of TGIF mRNA and protein in A549 and H1299 cell lines than that in 16HBE cell. Increased expressions of TGIF protein and mRNA were observed in 16HBE cells induced by BaP treatment as compared to those in solvent control group. We observed significantly higher levels of TGIF mRNA and protein in 16HBE-BaP cells than that in 16HBE-control cells. We observed significantly higher levels of TGIF mRNA and protein in mice lung tissues treated with BaP than that in control group. Our results suggested that elevated expression of TGIF was involved in lung carcinogenesis.
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Metadata
Title
Elevated expression of TGIF is involved in lung carcinogenesis
Authors
Yadong Wang
Haiyu Wang
Huiyan Gao
Bing Xu
Wenlong Zhai
Jiangmin Li
Congke Zhang
Publication date
01-12-2015
Publisher
Springer Netherlands
Published in
Tumor Biology / Issue 12/2015
Print ISSN: 1010-4283
Electronic ISSN: 1423-0380
DOI
https://doi.org/10.1007/s13277-015-3615-8

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