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Graefe's Archive for Clinical and Experimental Ophthalmology

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01-10-2018 | Basic Science

Effects of titanium dioxide nanoparticles on the inhibition of cellular activity in human Tenon’s fibroblasts under UVA exposure

Authors: Seung-Uk Lee, Ji-Eun Lee, Su-Jin Kim, Jong-Soo Lee

Published in: Graefe's Archive for Clinical and Experimental Ophthalmology | Issue 10/2018

Abstract

Purpose

To investigate the effect of titanium dioxide (TiO₂) nanoparticles on the inhibition of the in vitro cellular activity of human Tenon’s fibroblasts (HTFs) under UVA exposure.

Methods

The effects of TiO₂ nanoparticles on human Tenon’s fibroblasts were evaluated after 1, 4, 6, and 24 h of exposure to UVA at levels of 2.5, 5.0, and 10 J/cm². The methyl thiazolyl tetrazolium (MTT) assay was performed to measure the suppression of cellular metabolic activity. The lactate dehydrogenase (LDH) assay was performed to determine the extent of cell membrane damage. Flow cytometric analysis and inverted phase-contrast and electron microscopy were performed. The scratch wound assay was performed to visualize suppression of cellular migration.

Results

MTT assay values were similar between the UVA-exposed groups and the control group without UVA exposure. However, the combined exposure of TiO₂ nanoparticles and UVA exposure induced significant dose-dependent inhibition of cellular viability and damage to HTFs, especially at concentrations of TiO₂ equal to or greater than 100 μg/mL and 2.5 J/cm² of UVA irradiation. Changes in cellular morphology increased in a dose-dependent pattern with a TiO₂ concentration greater than 100 μg/mL under UVA exposure. At a TiO₂ concentration of 150 μg/mL, damage to the cellular morphology of the HTFs was significantly increased, and nanoparticles were seen inside of the cytoplasm in the affected HTFs exposed to UVA. There was a significant reduction of cellular migration at TiO₂ concentrations higher than 150 μg/mL.

Conclusion

TiO₂ nanoparticles inhibited the cellular activity of HTFs under UVA irradiation and showed potential for use to prevent the wound scarring of Tenon’s fibroblasts. Further studies will be necessary to determine the optimal concentration of TiO₂ nanoparticles and UVA exposure dose for clinical applications.

Lockwood A, Brocchini S, Khaw PT (2013) New developments in the pharmacological modulation of wound healing after glaucoma filtration surgery. Curr Opin Pharmacol 13(1):65–71. https://doi.org/10.1016/j.coph.2012.10.008 CrossRefPubMed

Jampel HD, Pasquale LR, Dibernardo C (1992) Hypotony maculopathy following trabeculectomy with mitomycin C. Arch Ophthalmol 110(8):1049–1050CrossRefPubMed

Chawla A, Mercieca K, Fenerty C, Jones NP (2013) Outcomes and complications of trabeculectomy enhanced with 5-fluorouracil in adults with glaucoma secondary to uveitis. J Glaucoma 22(8):663–666. https://doi.org/10.1097/IJG.0b013e318255dc07 CrossRefPubMed

Higginbotham EJ, Stevens RK, Musch DC, Karp KO, Lichter PR, Bergstrom TJ, Skuta GL (1996) Bleb-related endophthalmitis after trabeculectomy with mitomycin C. Ophthalmology 103(4):650–656CrossRefPubMed

Akova YA, Bulut S, Dabil H, Duman S (1999) Late bleb-related endophthalmitis after trabeculectomy with mitomycin C. Ophthalmic Surg Lasers 30(2):146–151PubMed

Stamper RL, McMenemy MG, Lieberman MF (1992) Hypotonous maculopathy after trabeculectomy with subconjunctival 5-fluorouracil. Am J Ophthalmol 114(5):544–553CrossRefPubMed

Darian-Smith E, Toh T (2014) Corneal decompensation following bleb needling with 0.01% mitomycin C: a case study. Clin Exp Ophthalmol 42(9):901–902. https://doi.org/10.1111/ceo.12345 CrossRefPubMed

Chen X, Selloni A (2014) Introduction: titanium dioxide (TiO2) nanomaterials. Chem Rev 114(19):9281–9282. https://doi.org/10.1021/cr500422r CrossRefPubMed

Chen X, Mao SS (2007) Titanium dioxide nanomaterials: synthesis, properties, modifications, and applications. Chem Rev 107(7):2891–2959. https://doi.org/10.1021/cr0500535 CrossRefPubMed

10.

Kang SJ, Lee YJ, Kim BM, Choi YJ, Chung HW (2011) Cytotoxicity and genotoxicity of titanium dioxide nanoparticles in UVA-irradiated normal peripheral blood lymphocytes. Drug Chem Toxicol 34(3):277–284. https://doi.org/10.3109/01480545.2010.546800 CrossRefPubMed

11.

Tu M, Huang Y, Li HL, Gao ZH (2012) The stress caused by nitrite with titanium dioxide nanoparticles under UVA irradiation in human keratinocyte cell. Toxicology 299(1):60–68. https://doi.org/10.1016/j.tox.2012.05.011 CrossRefPubMed

12.

Sayes CM, Wahi R, Kurian PA, Liu Y, West JL, Ausman KD, Warheit DB, Colvin VL (2006) Correlating nanoscale titania structure with toxicity: a cytotoxicity and inflammatory response study with human dermal fibroblasts and human lung epithelial cells. Toxicol Sci 92(1):174–185. https://doi.org/10.1093/toxsci/kfj197 CrossRefPubMed

13.

Yin JJ, Liu J, Ehrenshaft M, Roberts JE, Fu PP, Mason RP, Zhao B (2012) Phototoxicity of nano titanium dioxides in HaCaT keratinocytes--generation of reactive oxygen species and cell damage. Toxicol Appl Pharmacol 263(1):81–88. https://doi.org/10.1016/j.taap.2012.06.001 CrossRefPubMedPubMedCentral

14.

Sanders K, Degn LL, Mundy WR, Zucker RM, Dreher K, Zhao B, Roberts JE, Boyes WK (2012) In vitro phototoxicity and hazard identification of nano-scale titanium dioxide. Toxicol Appl Pharmacol 258(2):226–236. https://doi.org/10.1016/j.taap.2011.10.023 CrossRefPubMed

15.

Seo JW, Chung H, Kim MY, Lee J, Choi IH, Cheon J (2007) Development of water-soluble single-crystalline TiO2 nanoparticles for photocatalytic cancer-cell treatment. Small 3(5):850–853. https://doi.org/10.1002/smll.200600488 CrossRefPubMed

16.

Cai R, Kubota Y, Shuin T, Sakai H, Hashimoto K, Fujishima A (1992) Induction of cytotoxicity by photoexcited TiO2 particles. Cancer Res 52(8):2346–2348PubMed

17.

Adams LK, Lyon DY, McIntosh A, Alvarez PJ (2006) Comparative toxicity of nano-scale TiO2, SiO2 and ZnO water suspensions. Water Sci Technol 54(11–12):327–334CrossRefPubMed

18.

Hirakawa K, Mori M, Yoshida M, Oikawa S, Kawanishi S (2004) Photo-irradiated titanium dioxide catalyzes site specific DNA damage via generation of hydrogen peroxide. Free Radic Res 38(5):439–447CrossRefPubMed

19.

Desmouliere A, Redard M, Darby I, Gabbiani G (1995) Apoptosis mediates the decrease in cellularity during the transition between granulation tissue and scar. Am J Pathol 146(1):56–66PubMedPubMedCentral

20.

Liao CM, Chiang YH, Chio CP (2009) Assessing the airborne titanium dioxide nanoparticle-related exposure hazard at workplace. J Hazard Mater 162(1):57–65. https://doi.org/10.1016/j.jhazmat.2008.05.020 CrossRefPubMed

21.

Rehn B, Seiler F, Rehn S, Bruch J, Maier M (2003) Investigations on the inflammatory and genotoxic lung effects of two types of titanium dioxide: untreated and surface treated. Toxicol Appl Pharmacol 189(2):84–95CrossRefPubMed

Title: Effects of titanium dioxide nanoparticles on the inhibition of cellular activity in human Tenon’s fibroblasts under UVA exposure
Authors: Seung-Uk Lee
Ji-Eun Lee
Su-Jin Kim
Jong-Soo Lee
Publication date: 01-10-2018
Publisher: Springer Berlin Heidelberg
Published in: Graefe's Archive for Clinical and Experimental Ophthalmology / Issue 10/2018
Print ISSN: 0721-832X
Electronic ISSN: 1435-702X
DOI: https://doi.org/10.1007/s00417-018-4091-9

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Springer Medicine

Effects of titanium dioxide nanoparticles on the inhibition of cellular activity in human Tenon’s fibroblasts under UVA exposure

Abstract

Purpose

Methods

Results

Conclusion

Keynote webinar | Spotlight on medication adherence

Springer Medicine

Abstract

Purpose

Methods

Results

Conclusion

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