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Aesthetic Plastic Surgery

Published in:

01-04-2017 | Original Article

Effects of Platelet-Rich Plasma on Tissue Expansion in Rabbits

Authors: Wang Jinming, Liu Caiyue, Wu Baojin, Liu Antang, Zhang Yingfan, Wang Hui, Zhu Lie, Jiang Hua

Published in: Aesthetic Plastic Surgery | Issue 2/2017

Abstract

Background

Tissue expansion has been widely applied in clinic practice. However, the expanded skin is always insufficient for clinical use and how to promote tissue expansion is still a challenge. Platelet-rich plasma (PRP) contains multiple tissue growth factors and has been widely utilized in hard- and soft-tissue augmentation. However, its feasibility to promote tissue expansion has not been explored. The purpose of this study was to evaluate the possible effect of PRP in tissue expansion.

Methods

Tissue expanders were implanted in the dorsal sides of rabbit ears, and the left and right side were for the PRP group and the control group randomly. The PRP group was injected with 1.0 mL of autologous PRP in the expansion skin and the control group with the same volume of saline solution. The expanders were inflated regularly by injecting saline solution under the same pressure. After the inflation phase, the expansion skin was harvested and the inflation volume, gross specimen, and histological characteristics were assayed and evaluated.

Results

The mean inflation volume of the PRP group was significantly higher than that of the control (p < 0.05). H&E staining of expanded skin sections showed more epidermal cell layers and thicker skin in the PRP group than that in the control group. There were significantly more capillaries in the expanded skin in the PRP group than those from the control group by IHC staining with its marker anti-CD31. A higher level of mRNA expression of VEGF was observed in the PRP group by real-time PCR.

Conclusions

The present study suggests that PRP plays a pivotal role in tissue expansion and skin proliferation.

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Neumann CG (1946, 1957). The expansion of an area of skin by progressive distention of a subcutaneous balloon; use of the method for securing skin for subtotal reconstruction of the ear. Plast Reconst Surg 19: 124–130

Radovan C (1982) Breast reconstruction after mastectomy using the temporary expander. Plast Reconstr Surg 69:195–208CrossRefPubMed

Spencer JR (1992) Experience with novel uses of tissue expanders in burn reconstruction of the face and neck. Ann Plast Surg 28(5):453–464CrossRef

Bozkurt A, Groger A, O’Dey D et al (2008) Retrospective analysis of tissue expansion in reconstructive burn surgery: evaluation of complication rates. Burns 34:1113–1118CrossRefPubMed

Di Mascio D, Castagnetti F, Mazzeo F et al (2006) Overexpansion technique in burn scar management. Burns 32:490–498CrossRefPubMed

Vergnes P, Taieb A, Maleville J et al (1993) Repeated skin expansion for excision of congenital giant nevi in infancy and childhood. Plast Reconstr Surg 91:450–455CrossRefPubMed

Lee P, Squier CA, Bardach J (1985) Enhancement of tissue expansion by anticontractile agents. Plast Reconstr Surg 76:604–610CrossRefPubMed

Yang M, Li Q, Sheng L et al (2011) Bone marrow-derived mesenchymal stem cells transplantation accelerates tissue expansion by promoting skin regeneration during expansion. Ann Surg 253:202–209CrossRefPubMed

Matt BH, Squier CA, Kelly KM et al (1990) Enhancement of expansion of guinea pig skin by local delivery of an anticontractile agent using a new bilumen expander. Ann Plast Surg 24:335–341CrossRefPubMed

10.

Raposio E, Santi PL (1999) Topical application of DMSO as an adjunct to tissue expansion for breast reconstruction. Br J Plast Surg 52:194–197CrossRefPubMed

11.

Marx RE (2004) Platelet-rich plasma: evidence to support its use. J Oral Maxillofac Surg 62:489–496CrossRefPubMed

12.

Ng F, Boucher S, Koh S, Sastry KS, Chase L, Lakshmipathy U et al (2008) PDGF, TGF-beta, and FGF signaling is important for differentiation and growth of mesenchymal stem cells (MSCs): transcriptional profiling can identify markers and signaling pathways important in differentiation of MSCs into adipogenic, chondrogenic, and osteogenic lineages. Blood 112:295–307CrossRefPubMed

13.

Garg AK, Gargenese D, Peace I (2000) Using platelet-rich plasma to develop an autologous membrane for growth factor delivery in dental implant therapy. Dent Implantol Update 11:41–44PubMed

14.

Abuzeni PZ, Alexander RW (2001) Enhancement of autologous fat transplantation with platelet rich plasma. Am J Cosmet Surg 18:59–70

15.

Man D, Plosker H, Winland-Brown JE (2001) The use of autologous platelet-rich plasma (platelet gel) and autologous platelet-poor plasma (fibrin glue) in cosmetic surgery. Plast Reconstr Surg 107:229–239CrossRefPubMed

16.

Powell DM, Chang E, Farrior EH (2001) Recovery from deep-plane rhytidectomy following unilateral wound treatment with autologous platelet gel. Arch Facial Plant Surg 3:245–250CrossRef

17.

Kassolis JD, Reynolds MA (2005) Evaluation of the adjunctive benefits of platelet rich plasma in subantral sinus segmentation. J Craniofac Surg 16:280–287CrossRefPubMed

18.

Sadati KS, Corrado AC, Alexander RW (2006) Platelet-rich plasma (PRP) utilized to promote greater graft volume retention in autologous fat grafting. Am J Cosmet Surg 23:203–211CrossRef

19.

Landesberg R, Roy M, Glickman RS (2000) Quantification of growth factor levels using a simplified method of platelet-rich plasma gel preparation. J Oral Maxillofac Surg 58(3):297–300CrossRefPubMed

20.

Castello JR, Garro L, Najera A, Mirelis E, Sanchez-Olaso A, Barros J (2000) Immediate breast reconstruction in two stages using anatomical tissue expansion. Scand J Plast Reconstr Surg Hand Surg 34:667–671

21.

Argenta LC, Watanabe MJ, Grabb WC (1983) The use of tissue expansion in head and neck reconstruction. Ann Plast Surg 11:31–37CrossRefPubMed

22.

Liu X, Zhang Q, Quan Y, Xie Y, Shi L (2010) Bilateral microtia reconstruction. J Plast Reconstr Aesthet Surg 63(8):1275–1278CrossRefPubMed

23.

Kheradmand AA, Garajei A, Motamedi MH (2011) Nasal reconstruction: experience using tissue expansion and forehead flap. J Oral Maxillofac Surg 69(5):1478–1484CrossRefPubMed

24.

Johnson T, Lowe L, Brown M (1993) Histology and physiology of tissue expansion. J Dermatol Surg Oncol 19:1074–1078CrossRefPubMed

25.

Mazzucco L, Medici D, Serra M et al (2004) The use of autologous platelet gel to treat difficult-to-heal wounds: a pilot study. Transfusion 44:1013–1018CrossRefPubMed

26.

Pietramaggiori G, Kaipainen A, Czeczuga JM et al (2006) Freezedried platelet-rich plasma shows beneficial healing properties in chronic wounds. Wound Rep Reg 14:573–580CrossRef

27.

Sugimori E, Shintani S, Ishikawa K et al (2006) Effect of apatite foam combined with platelet-rich plasma on regeneration of bone defects. Dent Mater J 25:591–596CrossRefPubMed

28.

29.

Bhanot S, Alex JC (2002) Current applications of platelet gels in facial plastic surgery. Facial Plast Surg 18:27–33CrossRefPubMed

30.

Powell DM, Chang E, Farrior EH (2001) Recovery from deep-plane rhytidectomy following unilateral wound treatment with autologous platelet gel. Arch Facial Plast Surg 3:245–250CrossRefPubMed

31.

Hosgood G (1993) Wound healing: the role of platelet-derived growth factor and transforming growth factor beta. Vet Surg 22:490–495CrossRefPubMed

32.

Christgau M, Moder D, Hiller KA, Dada A, Schmitz G, Schmalz G (2006) Growth factors and cytokines in autologous platelet concentrate and their correlation to periodontal regeneration outcomes. J Periodontol 33:837–845CrossRef

33.

Cho JM, Lee YH, Baek RM, Lee SW (2011) Effect of platelet-rich plasma on ultraviolet b-induced skin wrinkles in nude mice. J Plast Reconstr Aesthet Surg 64(2):e31–e39CrossRefPubMed

34.

de Vos RJ (2016) Does platelet-rich plasma increase tendon metabolism? Adv Exp Med Biol 920:263–273CrossRefPubMed

35.

Yu TY, Pang JH, Wu KP et al (2015) Platelet-rich plasma increases proliferation of tendon cells by modulating Stat3 and p27 to up-regulate expression of cyclins and cyclin-dependent kinases. Cell Prolif 48(4):413–420CrossRefPubMed

36.

Zhang L, Chen S, Chang P et al (2016) Harmful effects of leukocyte-rich platelet-rich plasma on rabbit tendon stem cells in vitro. Am J Sports Med 44(8):1941–1951CrossRefPubMed

37.

Flamme I, von Reutern M, Drexler HC, Syed-Ali S, Risau W (1995) Overexpression of vascular endothelial growth factor in the avian embryo induces hypervascularization and increased vascular permeability without alterations of embryonic pattern formation. Dev Biol 171:399–414CrossRefPubMed

Title: Effects of Platelet-Rich Plasma on Tissue Expansion in Rabbits
Authors: Wang Jinming
Liu Caiyue
Wu Baojin
Liu Antang
Zhang Yingfan
Wang Hui
Zhu Lie
Jiang Hua
Publication date: 01-04-2017
Publisher: Springer US
Published in: Aesthetic Plastic Surgery / Issue 2/2017
Print ISSN: 0364-216X
Electronic ISSN: 1432-5241
DOI: https://doi.org/10.1007/s00266-017-0797-z

Keynote webinar | Spotlight on medication adherence

Springer Medicine

Effects of Platelet-Rich Plasma on Tissue Expansion in Rabbits

Abstract

Background

Methods

Results

Conclusions

No Level Assigned

Keynote webinar | Spotlight on medication adherence

Springer Medicine

Abstract

Background

Methods

Results

Conclusions

No Level Assigned

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