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Journal of Cardiothoracic Surgery

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Open Access 01-12-2010 | Research article

Effects of inosine on reperfusion injury after cardiopulmonary bypass

Authors: Gábor Veres, Tamás Radovits, Leila Seres, Ferenc Horkay, Matthias Karck, Gábor Szabó

Published in: Journal of Cardiothoracic Surgery | Issue 1/2010

Abstract

Objective

Inosine, a break-down product of adenosine has been recently shown to exert inodilatory and anti-inflammatory properties. Furthermore inosine might be a key substrate of pharmacological post-conditioning. In the present pre-clinical study, we investigated the effects of inosine on cardiac function during reperfusion in an experimental model of cardioplegic arrest and extracorporal circulation.

Methods

Twelve anesthetized dogs underwent hypothermic cardiopulmonary bypass. After 60 minutes of hypothermic cardiac arrest, reperfusion was started after application of either saline vehicle (control, n = 6), or inosine (100 mg/kg, n = 6). Left ventricular end-systolic pressure volume relationship (ESPVR) was measured by a combined pressure-volume-conductance catheter at baseline and after 60 minutes of reperfusion. Left anterior descendent coronary blood flow (CBF), endothelium-dependent vasodilatation to acetylcholine (ACh) and endothelium-independent vasodilatation to sodium nitroprusside (SNP) were also determined.

Results

The administration of inosine led to a significantly better recovery (given as percent of baseline) of ESPVR 90 ± 9% vs. 46 ± 6%, p < 0.05. CBF and was also significantly higher in the inosine group (56 ± 8 vs. 23 ± 4, ml/min, p < 0.05). While the vasodilatatory response to SNP was similar in both groups, ACh resulted in a significantly higher increase in CBF (58 ± 6% vs. 25 ± 5%, p < 0.05) in the inosine group.

Conclusions

Application of inosine improves myocardial and endothelial function after cardiopulmonary bypass with hypothermic cardiac arrest.

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Title: Effects of inosine on reperfusion injury after cardiopulmonary bypass
Authors: Gábor Veres
Tamás Radovits
Leila Seres
Ferenc Horkay
Matthias Karck
Gábor Szabó
Publication date: 01-12-2010
Publisher: BioMed Central
Published in: Journal of Cardiothoracic Surgery / Issue 1/2010
Electronic ISSN: 1749-8090
DOI: https://doi.org/10.1186/1749-8090-5-106

Keynote webinar | Spotlight on medication adherence

Springer Medicine

Effects of inosine on reperfusion injury after cardiopulmonary bypass

Abstract

Objective

Methods

Results

Conclusions

Keynote webinar | Spotlight on medication adherence

Springer Medicine

Abstract

Objective

Methods

Results

Conclusions

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