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01-01-2010 | Original Article

Effects of Helicobacter pylori Infection on the Expressions of Bax and Bcl-2 in Patients with Chronic Gastritis and Gastric Cancer

Authors: Waldemar Bartchewsky Jr, Mariana R. Martini, Aline C. Squassoni, Marisa C. Alvarez, Marcelo S. P. Ladeira, Daisy M. F. Salvatore, Miriam A. Trevisan, José Pedrazzoli Jr, Marcelo L. Ribeiro

Published in: Digestive Diseases and Sciences | Issue 1/2010

Abstract

The aim of the present study is to evaluate the influence of Helicobacter pylori on Bax and Bcl-2 mRNA and protein levels in patients with chronic gastritis and gastric cancer. The study included 217 patients, of which 26 were uninfected; 127 had chronic gastritis and were H. pylori-positive, and 64 had gastric cancer. Bacterial genotypes were evaluated by PCR, and the expression values were determined by quantitative real-time PCR and immunohistochemistry. Our data showed that the up-regulationary effects of H. pylori infection on the pro-apoptotic gene, Bax, were stronger than its induction of Bcl-2; this effect may increase apoptosis in patients with chronic gastritis. In patients with gastric cancer, the up-regulation of the anti-apoptotic gene, Bcl-2, counteracted the pro-apoptotic effects of Bax, leading to a deregulation of apoptosis-associated gene expression, favoring cell proliferation. Thus, the disturbance in Bax and Bcl-2 balance, induced by H. pylori, might be important in gastric cancer development.

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Title: Effects of Helicobacter pylori Infection on the Expressions of Bax and Bcl-2 in Patients with Chronic Gastritis and Gastric Cancer
Authors: Waldemar Bartchewsky Jr
Mariana R. Martini
Aline C. Squassoni
Marisa C. Alvarez
Marcelo S. P. Ladeira
Daisy M. F. Salvatore
Miriam A. Trevisan
José Pedrazzoli Jr
Marcelo L. Ribeiro
Publication date: 01-01-2010
Publisher: Springer US
Published in: Digestive Diseases and Sciences / Issue 1/2010
Print ISSN: 0163-2116
Electronic ISSN: 1573-2568
DOI: https://doi.org/10.1007/s10620-008-0699-7

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