Skip to main content
Key Specialties
Cardiology Diabetology Endocrinology Gastroenterology Geriatrics and Gerontology Gynecology and Obstetrics Hematology Infectious Disease Internal Medicine Nephrology Neurology Oncology Pediatrics Respiratory Medicine Rheumatology Surgery
Congress Coverage
2024 ACC Congress 2023 ESMO Congress 2023 EASD Congress 2023 ERS Congress 2023 ESC Congress
Clinical Collections
Advances in Alzheimer’s

At a glance: The STEP trials

A round-up of the STEP phase 3 clinical trials evaluating semaglutide for weight loss in people with overweight or obesity.
ADVANCED SEARCH
Log in
Top
Lasers in Medical Science
Published in: Lasers in Medical Science 1/2014

01-01-2014 | Original Article

Effects of early and delayed laser application on nerve regeneration

Authors: Tuba Akgul, Murat Gulsoy, Halil O. Gulcur

Published in: Lasers in Medical Science | Issue 1/2014

Login to get access

Abstract

The aim of this study is to analyze the differences between early and delayed use of low-level laser therapy (LLLT) in functional and morphological recovery of the peripheral nerve. Thirty male Wistar rats were divided into three groups after the sciatic nerve was crushed: (1) control group without laser treatment, (2) early group with laser treatment started immediately after surgery and lasted 14 days, and (3) delayed group with laser treatment starting on the postoperative day 7 and lasted until day 21. A 650-nm diode laser (model: DH650-24-3(5), Huanic, China) with an output power of 25 mW exposed transcutaneously at three equidistant points on the surgical mark corresponding to the crushed nerve. The length of the laser application was calculated as 57 s to satisfy approximately 10 J/cm2. A Sciatic Functional Index (SFI) was used to evaluate functional improvement in groups at pre- and post-surgery (on days 7, 14, and 21). Compound action potential (CAP) was measured after the sacrifice and histological examination was performed for all groups. SFI results showed that there was no significant difference between groups at different days (p > 0.05). On the other hand, the latency of CAP decreased significantly (p < 0.05) in the delayed group. Histological examination confirmed that the number of mononuclear cells was lower (p < 0.05) in both early and delayed groups. In conclusion, results supported the hypothesis that LLLT could accelerate the rate of recovery of injured peripheral nerves in this animal model. Though both laser groups had positive outcomes, delayed group showed better recovery.
Literature
1.
Carlson NR (2010) Physiology of behavior. Pearson, Upper Saddle River, NJ
2.
Deumens R, Bozkurt A, Meek MF, Marcus MA, Joosten EA, Weis J, Brook GA (2010) Reparing injured peripheral nerves: bridging the gap. Prog Neurobiol 92:245–276PubMedCrossRef
3.
Brushart TM, Jari R, Verge V, Rohde C, Gordon T (2005) Electrical stimulation restores the specificity of sensory axon regeneration. Exp Neurol 194:221–229PubMedCrossRef
4.
Al-Majed AA, Neumann CM, Brushart TM, Gordon T (2000) Brief electrical stimulation promotes the speed and accuracy of motor axonal regeneration. J Neurosci 20:2602–2608PubMed
5.
Asensio-Pinilla E, Udina E, Jaramillo J, Navarro X (2009) Electrical stimulation combined with exercise increase axonal regeneration after peripheral nerve injury. Exp Neurol 219:258–265PubMedCrossRef
6.
La JL, Jalali S, Shami SA (2005) Morphological studies on crushed sciatic nerve of rabbits with electroacupuncture or diclofenac sodium treatment. Am J Chin Med 33:663–669PubMedCrossRef
7.
Gigo-Benato D, Geuna S, Rochkind S (2005) Phototherapy for enhancing peripheral nerve repair: a review of the literature. Muscle Nerve 31:694–701PubMedCrossRef
8.
Rochkind S (2009) Phototherapy in peripheral nerve regeneration: from basic science to clinical study. Neurosurg Focus 26
9.
Hrnjak M, Kuljic-Kapulica N, Budisin A, Giser A (1995) Stimulatory effect of low-power density He-Ne laser radiation on human fibroblasts in vitro. Vojnosanit Pregl 52(6):539–546PubMed
10.
Trelles MA, Mayayo E (1987) Bone fracture consolidates faster with low-power laser. Lasers Surg Med 7(1):36–45PubMedCrossRef
11.
Pinheiro AL, Gerbi ME (2006) Photoengineering of bone repair processes. Photomed Laser Surg 24(2):169–178PubMedCrossRef
12.
Xavier M, David DR, de Souza RA, Arrieiro AN, Miranda H, Santana ET, Silva JA Jr, Salgado MA, Aimbire F, Albertini R (2010) Anti-inflammatory effects of low-level light emitting diode therapy on Achilles tendinitis in rats. Lasers Surg Med 42(6):553–558PubMedCrossRef
13.
Soudry M, Franquin JC, Pourreau-Schreider N, Martin PM (1988) Effect of a helium-neon laser on cellular growth: an in vitro study of human gingival fibroblasts. J Biol Buccale 16(3):129–135PubMed
14.
Dall Agnol MA, Nicolau RA, de Lima CJ, Munin E (2009) Comparative analysis of coherent light action (laser) versus non-coherent light (light-emitting diode) for tissue repair in diabetic rats. Lasers Med Sci 24(6):909–916PubMedCrossRef
15.
Ihsan FRM (2005) Low-level laser therapy accelerates collateral circulation and enhances microcirculation. Photome Laser Surg 23:289–294CrossRef
16.
Shin DH, Lee E, Hyun JK, Lee SJ, Chang YP, Kim JW, Choi YS, Kwon BS (2003) Growth-associated protein-43 is elevated in the injured rat sciatic nerve after low power laser irradiation. Neurosci Lett 344:71–74PubMedCrossRef
17.
Eslamian L, Borzabadi-Farahani A, Hassanzadeh-Azhiri A, Badiee MR, Fekrazad R (2013) The effect of 810 nm low-level laser therapy on pain caused by orthodontic elearomic separator. Lasers Med Sci. doi:10.​1007/​s10103-012-1258-1
18.
Novoselova EG, Glushkova OV, Cherenkov DA, Chudnovsky VM, Fesenko EE (2006) Effects of low laser radiation on mice immunity. Photodermatol Photoimmunol Photomed 22(1):33–38PubMedCrossRef
19.
Serafim KG, Ramos Sde P, de Lima FM, Carandina M, Ferrari O, Dias IF, Toginho Filho Dde O, Siqueira CP (2012) Effects of 940 nm light-emitting diode (led) on sciatic nerve regeneration in rats. Lasers Med Sci 27:113–119PubMedCrossRef
20.
Barbosa RI, Marcolino AM, de Jesus Guirro RR, Mazzer N, Barbieri CH, de Cássia Registro Fonseca M (2010) Comparative effects of wavelengths of low-power laser in regeneration of sciatic nerve in rats following crushing lesion. Lasers Med Sci 25:423–430PubMedCrossRef
21.
Medalha CC, Di Gangi GC, Barbosa CB, Fernandes M, Aguiar O, Faloppa F, Leite VM, Renno AC (2012) Low-level laser therapy improves repair following complete resection of the sciatic nerve. Lasers Med Sci 27:629–635PubMedCrossRef
22.
dos Reis FA, Belchior AC, de Carvalho PT, da Silva BA, Pereira DM, Silva IS, Nicolau RA (2009) Effect of laser therapy (660 nm) on recovery of the sciatic nerve in rats after injury through neurotmesis followed by epineural anastomosis. Lasers Med Sci 24:741–747PubMedCrossRef
23.
Shamir MH, Rochkind S, Sandbank J, Alon M (2001) Double-blind randomized study evaluating regeneration of the rat transected sciatic nerve after suturing and postoperative low-power laser treatment. J Reconstr Microsurg 17:133–137PubMedCrossRef
24.
Mohammed IF, Al-Mustawfi N, Kaka LN (2007) Promotion of regenerative process in injured peripheral nerve induced by low-level laser therapy. Photomed Laser Surg 25:107–111PubMedCrossRef
25.
Chen YS, Hsu SF, Chiu CW, Lin JG, Chen CT, Yao CH (2005) Effects of low-power pulsed laser on peripheral nerve regeneration in rats. Microsurgery 25:83–89PubMedCrossRef
26.
Santos AP, Suaid CA, Xavier M, Yamane F (2011) Functional and morphometric differences between the early and delayed use of phototherapy in crushed median nerves of rats. Lasers Med Sci 27:479–486PubMedCrossRef
27.
Zochodne DW (2008) Neurobiology of peripheral nerve regeneration. Cambridge University Press, CambridgeCrossRef
28.
Rochkind S, Vogler I, Barr-Nea L (1990) Spinal cord response to laser treatment of injured peripheral nerve. Spine 15:6–10PubMedCrossRef
29.
Nissan M, Rochkind S, Ringel M (1998) Strain-gauged haemostatic forceps for clinical and experimental use. Med Biol Eng Comput 26:448–450CrossRef
30.
Varejão AS, Cabrita AM, Meek MF, Bulas-Cruz J, Melo-Pinto P, Raimondo S, Geuna S, Giacobini-Robecchi MG (2004) Functional and morphological assessment of standardized rat sciatic nerve crush injury with a non-serrated clamp. J Neurotrauma 21:1652–1670PubMed
31.
Chen LE, Seaber AV, Glisson RR, Davies H, Murrell GA, Anthony DC, Urbaniak JR (1992) The functional recovery of peripheral nerves following defined acute crush injuries. J Orthop Res 10:657–664PubMedCrossRef
32.
Mazzer PY, Barbieri CH, Mazzer N, Fazan VP (2008) Morphological and morphometric evaluation of experimental acute crush injuries of the sciatic nerve of rats. J Neurosci Methods 173:249–258PubMedCrossRef
33.
de Medinaceli L, Freed WJ, Wyatt RJ (1982) An index of the functional condition of rat sciatic nerve based on measurements made from walking tracks. Exp Neurol 77:634–643PubMedCrossRef
34.
Bain JR, Mackinnon SE, Hunter DA (1989) Functional evaluation of complete sciatic, peroneal, and posterior tibial nerve lesions in the rat. Plast Reconstr Surg 83:129–138PubMedCrossRef
35.
Anders JJ, Borke RC, Woolery SK, Van de Merwe WP (1993) Low power laser irradiation alters the rate of regeneration of the rat facial nerve. Lasers Surg Med 13:72–82PubMedCrossRef
36.
Gigo-Benato D, Russo TL, Tanaka EH, Assis L, Salvini TF, Parizotto NA (2010) Effects of 660 and 780 nm low-level laser therapy on neuromuscular recovery after crush injury in rat sciatic nerve. Lasers Med Sci 42:673–682
37.
Byrnes KR, Waynant RW, Ilev IK, Wu X, Barna L, Smith K, Heckert R, Gerst H, Anders JJ (2005) Light promotes regeneration and functional recovery and alter the immune response after spinal cord injury. Lasers Surg Med 36(3):171–185PubMedCrossRef
38.
Stoll G, Jander S, Myers RR (2002) Degeneration and regeneration of the peripheral nervous system: from Augustus Waller’s observations to neuroinflammation. J Periphe Nerv Syst 7:13–27CrossRef
39.
Nissan M, Rochkind S, Razon N, Bartal A (1986) HeNe laser irradiation delivered transcutaneously: its effect on the sciatic nerve of rats. Lasers Surg Med 6:435–438PubMedCrossRef
40.
Karu T, Pyatibrat L, Kalendo G (1995) Irradiation with He-Ne laser increases ATP level in cells cultivated in vitro. J Photochem Photobiol B 27:219–223PubMedCrossRef
41.
Fillipin LI, Mauriz JL, Vedovelli K, Moreira AJ, Zettler CG, Lech O, Marroni NP, González-Gallego J (2005) Low-level laser therapy (LLLT) prevents oxidative stress and reduces fibrosis in rat traumatized Achilles tendon. Lasers Surg Med 37(4):293–300PubMedCrossRef
42.
Rochkind S, Drory V, Alon M, Nissan M, Ouaknine GE (2007) Laser phototherapy (780 nm), a new modality in treatment of long-term incomplete peripheral nerve injury: a randomized double-blind placebo-controlled study. Photomed Laser Surg 25:436–442PubMedCrossRef
Metadata
Title
Effects of early and delayed laser application on nerve regeneration
Authors
Tuba Akgul
Murat Gulsoy
Halil O. Gulcur
Publication date
01-01-2014
Publisher
Springer London
Published in
Lasers in Medical Science / Issue 1/2014
Print ISSN: 0268-8921
Electronic ISSN: 1435-604X
DOI
https://doi.org/10.1007/s10103-013-1355-9

Other articles of this Issue 1/2014

Lasers in Medical Science 1/2014 Go to the issue

Original Article

Temperature imaging of laser-induced thermotherapy (LITT) by MRI: evaluation of different sequences in phantom

Original Article

Effects of low-power LED and therapeutic ultrasound in the tissue healing and inflammation in a tendinitis experimental model in rats

Original Article

Effect of low-level laser therapy on types I and III collagen and inflammatory cells in rats with induced third-degree burns

Original Article

Character of skin on photo-thermal response and its regeneration process using second-harmonic generation microscopy

Original Article

An in vitro evaluation of the responses of human osteoblast-like SaOs-2 cells to SLA titanium surfaces irradiated by erbium:yttrium–aluminum–garnet (Er:YAG) lasers

Original Article

Effect of 830-nm diode laser irradiation on human sperm motility