Skip to main content
Key Specialties
Cardiology Diabetology Endocrinology Gastroenterology Geriatrics and Gerontology Gynecology and Obstetrics Hematology Infectious Disease Internal Medicine Nephrology Neurology Oncology Pediatrics Respiratory Medicine Rheumatology Surgery
Congress Coverage
2024 ACC Congress 2023 ESMO Congress 2023 EASD Congress 2023 ERS Congress 2023 ESC Congress
Clinical Collections
Advances in Alzheimer’s

At a glance: The STEP trials

A round-up of the STEP phase 3 clinical trials evaluating semaglutide for weight loss in people with overweight or obesity.
ADVANCED SEARCH
Log in
Top
BMC Complementary Medicine and Therapies
Published in: BMC Complementary Medicine and Therapies 1/2018

Open Access 01-12-2018 | Research article

Effects of different extracts of curcumin on TPC1 papillary thyroid cancer cell line

Authors: Angelica Perna, Antonio De Luca, Laura Adelfi, Tammaro Pasquale, Bruno Varriale, Teresa Esposito

Published in: BMC Complementary Medicine and Therapies | Issue 1/2018

Login to get access

Abstract

Background

The thyroid gland is one of the largest endocrine glands in the body. The vast majority of TCs (> 90%) originate from follicular cells and are defined as differentiated thyroid cancers (DTC) and the two histological subtypes are the papillary TC with its variants and the follicular TC. Curcumin possesses a wide variety of biological functions, and thanks to its properties, it has gained considerable attention due to its profound medicinal values (Prasad, Gupta, Tyagi, and Aggarwal, Biotechnol Adv 32:1053–1064, 2014). We have undertaken the present work in order to define the possible role of curcumin in modulating the genetic expression of cell markers and to understand the effectiveness of this nutraceutical in modulating the regression of cancer phenotype.

Methods

As a template we used the TPC-1 cells treated with the different extracts of turmeric, and examined the levels of expression of different markers (proliferative, inflammatory, antioxidant, apoptotic).

Results

Treatment with the three different curcumin extracts displays anti-inflammatory, antioxidant properties and it is able to influence cell cycle with slightly different effects upon the extracts. Furthermore curcumin is able to influence cell metabolic activity vitality.

Conclusions

In conclusion curcumin has the potential to be developed as a safe therapeutic but further studies are needed to verify its antitumor ability in vivo.
Literature
1.
Cardone A, Angelini F, Esposito T, Comitato R, Varriale B. The expression of androgen receptor messenger RNA is regulated by tri-iodothyronine in lizard testis. J Steroid Biochem Mol Biol. 2000;72(3):133–41.CrossRefPubMed
2.
Esposito T, Astore E, Cardone A, Angelini F, Varriale B. Regulation of androgen receptor mRNA expression in primary culture of Harderian gland cells: cross-talk between steroid hormones. Comp Biochem Physiol B: Biochem Mol Biol. 2002;132(1):97–105.CrossRef
3.
Santangelo G, Pellino G, De Falco N, Colella G, D'Amato S, Maglione MG, De Luca R, Canonico S, De Falco M. Prevalence, diagnosis and management of ectopic thyroid glands. Int J Surg. 2016;28(Supplement 1):S1–6.CrossRefPubMed
4.
Leite AKN, Cavalheiro BG, Kulcsar MA, Hoff AO, Brandão LG, Cernea CR, Matos LL. Deaths related to differentiated thyroid cancer: a rare but real event. Arch Endocrinol Metab. 2017;61:222–7.CrossRefPubMed
5.
Repplinger D, Bargren A, Zhang YW, Adler JT, Haymart M, Chen H. Is Hashimoto's thyroiditis a risk factor for papillary thyroid cancer? J Surg Res. 2008;150(1):49–52.CrossRefPubMed
6.
Esposito T, Varriale B: Correlation between Thyroid Endocrine Status, Change in Body Weight and Macular Thickness in Obese Patients. General Medicine: Open Access. 2015;3:5–8.
7.
Messina G, Monda V, Moscatelli F: Role of Orexin System in Obesity. Biology and Medicine. 2015;3:5–8.
8.
9.
Prasad S, Gupta SC, Tyagi AK, Aggarwal BB. Curcumin, a component of golden spice: from bedside to bench and back. Biotechnol Adv. 2014;32(6):1053–64.CrossRefPubMed
10.
Kunnumakkara AB, Bordoloi D, Padmavathi G, Monisha J, Roy NK, Prasad S, Aggarwal BB. Curcumin, the golden nutraceutical: multitargeting for multiple chronic diseases. Br J Pharmacol. 2017;174(11):1325–48.CrossRefPubMed
11.
Kotecha R, Takami A, Espinoza JL.: Dietary phytochemicals and cancer chemoprevention: a review of the clinical evidence. Oncotarget 2016;7(32):52517–29.
12.
Kashat L, So AKC, Masui O, Wang XS, Cao J, Meng X, MacMillan C, Ailles LE, Siu KWM, Ralhan R, et al. Secretome-based identification and characterization of potential biomarkers in thyroid cancer. J Proteome Res. 2010;9(11):5757–69.CrossRefPubMed
13.
Schweppe RE, Klopper JP, Korch C, Pugazhenthi U, Benezra M, Knauf JA, Fagin JA, Marlow LA, Copland JA, Smallridge RC, et al. Deoxyribonucleic acid profiling analysis of 40 human thyroid cancer cell lines reveals cross-contamination resulting in cell line redundancy and misidentification. J Clin Endocrinol Metab. 2008;93(11):4331–41.CrossRefPubMedPubMedCentral
14.
Fagin JA, Mitsiades N. Molecular pathology of thyroid cancer: diagnostic and clinical implications. Best Pract Res Clin Endocrinol Metab. 2008;22(6):955–69.CrossRefPubMedPubMedCentral
15.
Zhang CY, Zhang L, Yu HX, Bao JD, Sun Z, Lu RR. Curcumin inhibits invasion and metastasis in K1 papillary thyroid cancer cells. Food Chem. 2013;139(1–4):1021–8.CrossRefPubMed
16.
Zhao X-C, Zhang L, Yu H-X, Sun Z, Lin X-F, Tan C, Lu R-R. Curcumin protects mouse neuroblastoma neuro-2A cells against hydrogen-peroxide-induced oxidative stress. Food Chem. 2011;129(2):387–94.CrossRef
17.
Esposito V, Perna A, Lucariello A, Carleo MA, Viglietti R, Sangiovanni V, Coppola N, Guerra G, De Luca A, Chirianni A. Different impact of antiretroviral drugs on bone differentiation in an in vitro model. J Cell Biochem. 2015;116(10):2188–94.CrossRefPubMed
18.
19.
Villeneuve NF, Sun Z, Chen W, Zhang DD. Nrf2 and p21 regulate the fine balance between life and death by controlling ROS levels. Cell Cycle. 2009;8(20):3255–6.CrossRefPubMedPubMedCentral
20.
Kim TH, Hur EG, Kang SJ, Kim JA, Thapa D, Lee YM, Ku SK, Jung Y, Kwak MK. NRF2 blockade suppresses colon tumor angiogenesis by inhibiting hypoxia-induced activation of HIF-1α. Cancer Res. 2011;71(6):2260–75.CrossRefPubMed
21.
Epelbaum R, Schaffer M, Vizel B, Badmaev V, Bar-Sela G. Curcumin and gemcitabine in patients with advanced pancreatic cancer. Nutr Cancer. 2010;62(8):1137–41.CrossRefPubMed
22.
Kanai M, Yoshimura K, Asada M, Imaizumi A, Suzuki C, Matsumoto S, Nishimura T, Mori Y, Masui T, Kawaguchi Y, et al. A phase I/II study of gemcitabine-based chemotherapy plus curcumin for patients with gemcitabine-resistant pancreatic cancer. Cancer Chemother Pharmacol. 2011;68(1):157–64.CrossRefPubMed
23.
Irving GRB, Howells LM, Sale S, Kralj-Hans I, Atkin WS, Clark SK, Britton RG, Jones DJL, Scott EN, Berry DP, et al. Prolonged biologically active colonic tissue levels of curcumin achieved after oral administration—a clinical pilot study including assessment of patient acceptability. Cancer Prev Res. 2013;6(2):119–28.CrossRef
24.
Greg M. Cole BT, and Sally A. Frautschy Neuroprotective effects of curcumin. Adv Exp Med Biol. 2007;595:197–212.
25.
Perry MC, Demeule M, Regina A, Moumdjian R, Beliveau R. Curcumin inhibits tumor growth and angiogenesis in glioblastoma xenografts. Mol Nutr Food Res. 2010;54(8):1192–201.PubMed
26.
Kunnumakkara AB, Anand P, Aggarwal BB. Curcumin inhibits proliferation, invasion, angiogenesis and metastasis of different cancers through interaction with multiple cell signaling proteins. Cancer Lett. 2008;269(2):199–225.CrossRefPubMed
27.
Sundram V, Chauhan SC, Ebeling M, Jaggi M. Curcumin attenuates β-catenin signaling in prostate cancer cells through activation of protein kinase D1. PLoS One. 2012;7(4):e35368.CrossRefPubMedPubMedCentral
28.
Wu G, Huang H, Abreu JG, He X. Inhibition of GSK3 phosphorylation of β-catenin via phosphorylated PPPSPXS motifs of Wnt Coreceptor LRP6. PLoS One. 2009;4(3):e4926.CrossRefPubMedPubMedCentral
29.
Vousden KH, Lu X. Live or let die: the cell's response to p53. Nat Rev Cancer. 2002;2(8):594–604.CrossRefPubMed
30.
31.
Ishizawa J, Kojima K, Hail N, Tabe Y, Andreeff M. Expression, function, and targeting of the nuclear exporter chromosome region maintenance 1 (CRM1) protein. Pharmacol Ther. 2015;153:25–35.CrossRefPubMedPubMedCentral
32.
Noske A, Weichert W, Niesporek S, Röske A, Buckendahl A-C, Koch I, Sehouli J, Dietel M, Denkert C. Expression of the nuclear export protein chromosomal region maintenance/exportin 1/Xpo1 is a prognostic factor in human ovarian cancer. Cancer. 2008;112(8):1733–43.CrossRefPubMed
33.
Weis K. Importins and exportins: how to get in and out of the nucleus. Trends Biochem Sci. 1998;23(5):185–9.CrossRefPubMed
34.
Wang X-J, Sun Z, Villeneuve NF, Zhang S, Zhao F, Li Y, Chen W, Yi X, Zheng W, Wondrak GT, et al. Nrf2 enhances resistance of cancer cells to chemotherapeutic drugs, the dark side of Nrf2. Carcinogenesis. 2008;29(6):1235–43.CrossRefPubMedPubMedCentral
35.
Moon EJ, Giaccia A. The dual roles of NRF2 in tumor prevention and progression: possible implications in cancer treatment. Free Radic Biol Med. 2015;0:292–9.CrossRef
36.
Shibata T, Ohta T, Tong KI, Kokubu A, Odogawa R, Tsuta K, Asamura H, Yamamoto M, Hirohashi S. Cancer related mutations in NRF2 impair its recognition by Keap1-Cul3 E3 ligase and promote malignancy. Proc Natl Acad Sci U S A. 2008;105(36):13568–73.CrossRefPubMedPubMedCentral
37.
Nioi P, Nguyen T. A mutation of Keap1 found in breast cancer impairs its ability to repress Nrf2 activity. Biochem Biophys Res Commun. 2007;362(4):816–21.CrossRefPubMed
38.
Stacy DR, Ely K, Massion PP, Yarbrough WG, Hallahan DE, Sekhar KR, Freeman ML. Increased expression of nuclear factor E2 p45-related factor 2 (NRF2) in head and neck squamous cell carcinomas. Head Neck. 2006;28(9):813–8.CrossRefPubMed
39.
van der Wijst MGP, Brown R, Rots MG. Nrf2, the master redox switch: the Achilles’ heel of ovarian cancer? Biochim Biophys Acta. 2014;1846(2):494–509.PubMed
40.
Jiang T, Chen N, Zhao F, Wang X-J, Kong B, Zheng W, Zhang DD. High levels of Nrf2 determine chemoresistance in type II endometrial cancer. Cancer Res. 2010;70(13):5486–96.CrossRefPubMedPubMedCentral
41.
Buommino E, Donnarumma G, Manente L, De Filippis A, Silvestri F, Iaquinto S, Tufano MA, De Luca A. The helicobacter pylori protein HspB interferes with Nrf2/Keap1 pathway altering the antioxidant response of Ags cells. Helicobacter. 2012;17(6):417–25.CrossRefPubMed
42.
Rajitha B, Nagaraju GP, Shaib WL, Alese OB, Snyder JP, Shoji M, Pattnaik S, Alam A, El-Rayes BF. Novel synthetic curcumin analogs as potent antiangiogenic agents in colorectal cancer. Mol Carcinog. 2017;56(1):288–99.CrossRefPubMed
43.
Jee S-H, Shen S-C, Kuo M-L, Tseng C-R, Chiu H-C. Curcumin induces a p53-dependent apoptosis in human basal cell carcinoma cells. J Investig Dermatol. 1998;111(4):656–61.CrossRefPubMed
44.
Watson JL, Greenshields A, Hill R, Hilchie A, Lee PW, Giacomantonio CA, Hoskin DW. Curcumin-induced apoptosis in ovarian carcinoma cells is p53-independent and involves p38 mitogen-activated protein kinase activation and downregulation of Bcl-2 and survivin expression and akt signaling. Mol Carcinog. 2010;49(1):13–24.PubMed
Metadata
Title
Effects of different extracts of curcumin on TPC1 papillary thyroid cancer cell line
Authors
Angelica Perna
Antonio De Luca
Laura Adelfi
Tammaro Pasquale
Bruno Varriale
Teresa Esposito
Publication date
01-12-2018
Publisher
BioMed Central
Published in
BMC Complementary Medicine and Therapies / Issue 1/2018
Electronic ISSN: 2662-7671
DOI
https://doi.org/10.1186/s12906-018-2125-9

Other articles of this Issue 1/2018

BMC Complementary Medicine and Therapies 1/2018 Go to the issue

Research article

Withania somnifera modulates cancer cachexia associated inflammatory cytokines and cell death in leukaemic THP-1 cells and peripheral blood mononuclear cells (PBMC’s)

Research article

Acanthus ebracteatus leaf extract provides neuronal cell protection against oxidative stress injury induced by glutamate

Research article

Brazilian propolis ethanol extract and its component kaempferol induce myeloid-derived suppressor cells from macrophages of mice in vivo and in vitro

Research article

Effect of tt-farnesol and myricetin on in vitro biofilm formed by Streptococcus mutans and Candida albicans

Research article

The mutagenic and antimutagenic activity of Sutherlandia frutescens extracts and marker compounds

Research article

A Lanosteryl triterpene from Protorhus longifolia augments insulin signaling in type 1 diabetic rats