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Journal of the International Society of Sports Nutrition

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Open Access 01-12-2016 | Research article

Effects of dietary Docosahexaenoic, training and acute exercise on lipid mediators

Authors: X. Capó, M. Martorell, A. Sureda, J. A. Tur, A. Pons

Published in: Journal of the International Society of Sports Nutrition | Issue 1/2016

Abstract

Background

Eicosanoids mediate initiation and resolution of inflammation. Our aim was evaluating the effects of training, exercise and docosahexaenoic (DHA) supplementation on plasma eicosanoids levels and peripheral blood mononuclear cells (PBMCs) eicosanoids production.

Methods

Fifteen male footballers were distributed to placebo and experimental groups. Experimental group consumed DHA-enriched beverage (1.16 g DHA/day) for 8 weeks, placebo group consumed a placebo beverage. Blood samples were taken before and after the nutritional intervention in basal conditions and 2 h after acute exercise.

Results

Training increased basal Prostaglandin E1 (PGE₁) plasma levels and PBMCs cyclooxygenase 2 (COX-2) protein levels in both groups, but COX-1 protein levels only in the experimental group. Acute exercise increased plasma PGE₂ and PBMCs active NFκβ levels. Lipopolysaccharide (LPS)-stimulated PBMCs increases eicosanoids production (PGE₁, PGE₂, RvD1) in both groups and increased LPS-stimulated PBMCs active NFκβ. DHA supplementation increased COX-2 levels but decreased LPS-stimulated PBMCs PGE₁ and PGE₂ production. Neither DHA supplementation nor acute exercise altered the expression of NFκβ, COX-2, 15-LOX2, 5-LOX, or IL-1β genes in PBMCs.

Conclusions

The increase of PGE₁ plasma levels after training promoted systemic anti-inflammatory and vasodilator environment. Exercise and DHA supplementation acted synergistically by increasing plasma PGE₂ with anti-inflammatory effects. Exercise primed PBMCs to enhance PGE₁, PGE₂ and RvD1 production in response to LPS.

Trial registration

The project was registered at ClinicalTrial.gov (NCT02177383).

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Title: Effects of dietary Docosahexaenoic, training and acute exercise on lipid mediators
Authors: X. Capó
M. Martorell
A. Sureda
J. A. Tur
A. Pons
Publication date: 01-12-2016
Publisher: BioMed Central
Published in: Journal of the International Society of Sports Nutrition / Issue 1/2016
Electronic ISSN: 1550-2783
DOI: https://doi.org/10.1186/s12970-016-0126-y

Keynote webinar | Spotlight on medication adherence

Springer Medicine

Effects of dietary Docosahexaenoic, training and acute exercise on lipid mediators

Abstract

Background

Methods

Results

Conclusions

Trial registration

Keynote webinar | Spotlight on medication adherence

Springer Medicine

Abstract

Background

Methods

Results

Conclusions

Trial registration

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