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Intensive Care Medicine
Published in: Intensive Care Medicine 4/2007

01-04-2007 | Experimental

Effects of a cantaloupe melon extract/wheat gliadin biopolymer during aortic cross-clamping

Authors: Jochen Kick, Balázs Hauser, Hendrik Bracht, Maura Albicini, Sükrü Öter, Florian Simon, Ulrich Ehrmann, Catherine Garrel, Jörn Sträter, Uwe B. Brückner, Xavier M. Leverve, Hubert Schelzig, Günter Speit, Peter Radermacher, Claus-Martin Muth

Published in: Intensive Care Medicine | Issue 4/2007

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Abstract

Objective

We previously reported in healthy volunteers that a cantaloupe melon extract chemically combined with wheat gliadin (melon extract/gliadin) and containing SOD, catalase and residual glutathione peroxidase (GPx), protected against DNA strand-break damage induced by hyperbaric oxygen (HBO), a well-established model of DNA damage resulting from oxidative stress. Aortic cross-clamping is a typical example of ischemia/reperfusion injury-related oxidative stress, and therefore we investigated whether this melon extract/gliadin would also reduce DNA damage after aortic cross-clamping and reperfusion.

Design

Prospective, randomized, controlled experimental study.

Setting

Animal laboratory.

Patients and participants

18 anesthetized, mechanically ventilated and instrumented pigs.

Interventions

After 14 days of oral administration of 1250 mg of the melon extract/gliadin (n = 9) or vehicle (n = 9), animals underwent 30 min of thoracic aortic cross-clamping and 4 h of reperfusion.

Measurements and results

Before clamping, immediately before declamping, and at 2 and 4 h of reperfusion, we measured blood isoprostane (immunoassay) and malondialdehyde concentrations (fluorimetric thiobarbituric acid test), SOD, catalase and GPx activities (spectrophotometric kits), NO formation (nitrate+nitrite; chemoluminescence), DNA damage in whole blood samples and isolated lymphocytes exposed to hyperbaric oxygen (comet assay). Organ function was also evaluated. Kidney and spinal cord specimen were analysed for apoptosis (TUNEL assay). The melon extract/gliadin blunted the DNA damage, reduced spinal cord apoptosis and attenuated NO release, however, without any effect on lipid peroxidation and organ function.

Conclusions

Pre-treatment with the oral melon extract/gliadin may be a therapeutic option to reduce oxidative cell injury affiliated with aortic cross-clamping.
Appendix
Available only for authorised users
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Metadata
Title
Effects of a cantaloupe melon extract/wheat gliadin biopolymer during aortic cross-clamping
Authors
Jochen Kick
Balázs Hauser
Hendrik Bracht
Maura Albicini
Sükrü Öter
Florian Simon
Ulrich Ehrmann
Catherine Garrel
Jörn Sträter
Uwe B. Brückner
Xavier M. Leverve
Hubert Schelzig
Günter Speit
Peter Radermacher
Claus-Martin Muth
Publication date
01-04-2007
Publisher
Springer-Verlag
Published in
Intensive Care Medicine / Issue 4/2007
Print ISSN: 0342-4642
Electronic ISSN: 1432-1238
DOI
https://doi.org/10.1007/s00134-006-0518-6

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