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Published in: Pediatric Nephrology 7/2003

01-07-2003 | Original Article

Effective chemical preservation of morphology of urinary erythrocytes

Authors: Olga Komarova, Wim van der Meer, Elena Levtchenko, Leo Monnens

Published in: Pediatric Nephrology | Issue 7/2003

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Abstract

Between 2 and 4 h after miction the morphology of urinary erythrocytes has changed, clouding the distinction between glomerular and non-glomerular bleeding in pediatric patients. Glomerular bleeding is characterized by microscopically visible alteration in urinary erythrocytes due to glomerular disease. Fixation by Cellfix, a formaldehyde-based fixative, allows the preservation of the morphology for at least 24 h and can be recommended for clinical practice. In our experience, thiomersal was not effective for preservation of the morphology of erythrocytes.
Literature
1.
go back to reference Rizzoni G, Braggion F, Zacchello G (1983) Evaluation of glomerular and non glomerular hematuria by phase contrast microscopy. J Pediatr 103:370–374PubMed Rizzoni G, Braggion F, Zacchello G (1983) Evaluation of glomerular and non glomerular hematuria by phase contrast microscopy. J Pediatr 103:370–374PubMed
2.
go back to reference Roth S, Renner R, Rathert P (1991) Microscopic hematuria: advances in identification of glomerular dysmorphic erythrocytes. J Urol 146:680–684PubMed Roth S, Renner R, Rathert P (1991) Microscopic hematuria: advances in identification of glomerular dysmorphic erythrocytes. J Urol 146:680–684PubMed
3.
go back to reference Anpalahan M, Birch D, Becker G (1994) Chemical preservation of urine sediment for phase contrast microscopic examination. Nephron 68:180–183PubMed Anpalahan M, Birch D, Becker G (1994) Chemical preservation of urine sediment for phase contrast microscopic examination. Nephron 68:180–183PubMed
4.
go back to reference Matsuyama T, Morita M, Ikeda M, Ishihara Y, Hiramoto R, Kamiyama Y, Honda M, Ito H (1997) Evaluation of preserved urinary red blood cells by light microscopy. Clin Nephrol 47:271–272PubMed Matsuyama T, Morita M, Ikeda M, Ishihara Y, Hiramoto R, Kamiyama Y, Honda M, Ito H (1997) Evaluation of preserved urinary red blood cells by light microscopy. Clin Nephrol 47:271–272PubMed
5.
go back to reference Rodriguez Moreno M, Rodriguez Moreno I, Leon M, Boy M, Agnieszka (1999) A new chemical preservative that permits analysis of urine sediment for light microscopic examination 12 h. after emission. Nephron 82:65–71PubMed Rodriguez Moreno M, Rodriguez Moreno I, Leon M, Boy M, Agnieszka (1999) A new chemical preservative that permits analysis of urine sediment for light microscopic examination 12 h. after emission. Nephron 82:65–71PubMed
6.
go back to reference Snoek B van der, Koene R (1997) Fixation of urinary sediment. Lancet 350:933–934 Snoek B van der, Koene R (1997) Fixation of urinary sediment. Lancet 350:933–934
7.
go back to reference Wandel E, Köhler H (1998) Acanthocytes in urinary sediment—a pathognomonic marker? Nephrol Dial Transplant 13:206–207 Wandel E, Köhler H (1998) Acanthocytes in urinary sediment—a pathognomonic marker? Nephrol Dial Transplant 13:206–207
Metadata
Title
Effective chemical preservation of morphology of urinary erythrocytes
Authors
Olga Komarova
Wim van der Meer
Elena Levtchenko
Leo Monnens
Publication date
01-07-2003
Publisher
Springer-Verlag
Published in
Pediatric Nephrology / Issue 7/2003
Print ISSN: 0931-041X
Electronic ISSN: 1432-198X
DOI
https://doi.org/10.1007/s00467-003-1149-6

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