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Journal of Assisted Reproduction and Genetics

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01-01-2013 | Embryo Biology

Effect of sperm entry on blastocyst development after in vitro fertilization and intracytoplasmic sperm injection — mouse model

Authors: Karolina Piotrowska-Nitsche, Anthony W. S. Chan

Published in: Journal of Assisted Reproduction and Genetics | Issue 1/2013

Abstract

Purpose

To investigate whether in vitro fertilization (IVF) and intracytoplasmic sperm injection (ICSI), influence the embryo’s development and its quality using the mouse as a model.

Methods

Assisted fertilization was performed using ICSI and IVF. Fluorescent beads were adhered to the fertilization cone or place of previous sperm injection in the natural mated (NM), IVF and ICSI embryos, respectively. Embryo examination was carried out at the two-cell and blastocyst stage to determine the position of fluorescent bead. Protein expression was detected by fluorescence immunocytochemical staining and confocal microscopic imaging of blastocysts.

Results

IVF and ICSI embryos developed at rates comparable to NM group. Embryos show similar expression patterns of two transcription factors, Oct4 and Cdx2. The most preferred place for spermatozoa attachment was the equatorial site of the egg, whether fertilization occurred in vitro or under natural conditions. We also link the sperm entry position (SEP) to embryo morphology and the number of cells at the blastocyst stage, with no influence of the method of fertilization.

Conclusions

IVF and ICSI, do not compromise in vitro pre-implantation development. Additional data, related to sperm entry, could offer further criteria to predict embryos that will implant successfully. Based on embryo morphology, developmental rate and protein expression level of key transcription factors, our results support the view that ART techniques, such as IVF and ICSI, do not perturb embryonic development or quality.

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Title: Effect of sperm entry on blastocyst development after in vitro fertilization and intracytoplasmic sperm injection — mouse model
Authors: Karolina Piotrowska-Nitsche
Anthony W. S. Chan
Publication date: 01-01-2013
Publisher: Springer US
Published in: Journal of Assisted Reproduction and Genetics / Issue 1/2013
Print ISSN: 1058-0468
Electronic ISSN: 1573-7330
DOI: https://doi.org/10.1007/s10815-012-9896-6

Keynote webinar | Spotlight on medication adherence

Springer Medicine

Effect of sperm entry on blastocyst development after in vitro fertilization and intracytoplasmic sperm injection — mouse model

Abstract

Purpose

Methods

Results

Conclusions

Keynote webinar | Spotlight on medication adherence

Springer Medicine

Abstract

Purpose

Methods

Results

Conclusions

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