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European Journal of Applied Physiology

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01-01-2016 | Original Article

Effect of regional muscle location but not adiposity on mitochondrial biogenesis-regulating proteins

Authors: Jesús Gustavo Ponce-González, Ignacio Ara, Steen Larsen, Borja Guerra, Jose A. L. Calbet, Jørn Wulff Helge

Published in: European Journal of Applied Physiology | Issue 1/2016

Abstract

Purpose

The aim of this study was to determine if the expression of the mitochondrial biogenesis-regulating proteins SIRT1, SIRT3 and PGC-1alpha in human skeletal muscle is influenced by adiposity.

Method

Twenty-nine male subjects were recruited into three groups: control (n = 10), obese (n = 10) and post-obese (n = 9). Intentionally, groups were matched by age, aerobic capacity and in addition the control and post-obese groups also by BMI. Muscle biopsies were obtained from the m. deltoid and vastus lateralis. PGC-1alpha, SIRT1 and SIRT3 protein expression was analyzed by Western blot.

Result

PGC-1alpha, SIRT1 and SIRT3 protein expression was similar regardless of the level of adiposity. Only a main effect of group on SIRT1 protein showed a trend toward higher expression in post-obese than control and obese (P = 0.09). Despite similar muscle fiber-type composition (previously reported), PGC-1alpha, SIRT1 and SIRT3 protein expression was higher in leg compared to arm muscle in all groups (P < 0.05).

Conclusion

This study shows that PGC-1alpha, SIRT1 and SIRT3 protein expression in basal conditions was not altered in humans with different levels of adiposity but similar aerobic capacity. The expression of PGC-1alpha, SIRT1 and SIRT3 was higher in vastus lateralis than in deltoid muscle, indicating that local rather than systemic factors prevail in regulating the level of expression of these proteins.

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Title: Effect of regional muscle location but not adiposity on mitochondrial biogenesis-regulating proteins
Authors: Jesús Gustavo Ponce-González
Ignacio Ara
Steen Larsen
Borja Guerra
Jose A. L. Calbet
Jørn Wulff Helge
Publication date: 01-01-2016
Publisher: Springer Berlin Heidelberg
Published in: European Journal of Applied Physiology / Issue 1/2016
Print ISSN: 1439-6319
Electronic ISSN: 1439-6327
DOI: https://doi.org/10.1007/s00421-015-3232-7

Keynote webinar | Spotlight on medication adherence

Springer Medicine

Effect of regional muscle location but not adiposity on mitochondrial biogenesis-regulating proteins

Abstract

Purpose

Method

Result

Conclusion

Keynote webinar | Spotlight on medication adherence

Springer Medicine

Abstract

Purpose

Method

Result

Conclusion

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