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BMC Oral Health

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Open Access 01-12-2016 | Research article

Effect of recombinant PDGF-BB on bone formation in the presence of β-tricalcium phosphate and bovine bone mineral matrix: a pilot study in rat calvarial defects

Authors: Eloá R. Luvizuto, Stefan Tangl, Toni Dobsak, Karoline Reich, Reinhard Gruber, Celso K. Sonoda, Roberta Okamoto

Published in: BMC Oral Health | Issue 1/2016

Abstract

Background

Supplementation of bone substitutes with recombinant platelet-derived growth factor-BB (PDGF-BB) can enhance bone regeneration. The aim of the study was to evaluate the effect of PDGF-BB on bone formation in the presence of β-tricalcium phosphate and bovine bone mineral matrix in a rat calvaria defect model.

Methods

The authors examined 5 mm rat calvarial defects treated with β-tricalcium phosphate (TCP) or demineralized bovine bone mineral (DBBM) with and without 0.3 mg/ml recombinant PDGF-BB. Calvaria defects were randomly divided into the following treatment groups (n = 5); TCP; TCP plus PDGF-BB; DBBM; DBBM plus PDGF-BB; and untreated empty control. After 45 days, bone formation was evaluated by histomorphometry and fluorescence microscopy.

Results

The authors report that the area of newly formed bone was similar between the empty controls and the two bone substitutes, TCP and DBBM. Supplementation of TCP and DBBM with PDGF-BB had no significant impact on bone formation. Fluorochrome staining revealed no visible changes in the pattern of bone formation in defects filled with TCP and DBBM, irrespective of PDGF-BB. Furthermore, supplementation with PDGF-BB did not influence biomaterial degradation.

Conclusions

The authors concluded that PDGF-BB had no impact on bone formation and degradation of bone substitutes in the respective rodent models. Thus, possible beneficial effects of PDGF-BB may require other model situations.

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Title: Effect of recombinant PDGF-BB on bone formation in the presence of β-tricalcium phosphate and bovine bone mineral matrix: a pilot study in rat calvarial defects
Authors: Eloá R. Luvizuto
Stefan Tangl
Toni Dobsak
Karoline Reich
Reinhard Gruber
Celso K. Sonoda
Roberta Okamoto
Publication date: 01-12-2016
Publisher: BioMed Central
Published in: BMC Oral Health / Issue 1/2016
Electronic ISSN: 1472-6831
DOI: https://doi.org/10.1186/s12903-016-0210-3

Keynote webinar | Spotlight on medication adherence

Springer Medicine

Effect of recombinant PDGF-BB on bone formation in the presence of β-tricalcium phosphate and bovine bone mineral matrix: a pilot study in rat calvarial defects

Abstract

Background

Methods

Results

Conclusions

Keynote webinar | Spotlight on medication adherence

Springer Medicine

Abstract

Background

Methods

Results

Conclusions

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