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Published in: European Journal of Applied Physiology 11-12/2016

01-12-2016 | Original Article

Effect of protein quality on recovery after intense resistance training

Authors: E. Rindom, M. H. Nielsen, K. Kececi, M. E. Jensen, K. Vissing, J. Farup

Published in: European Journal of Applied Physiology | Issue 11-12/2016

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Abstract

Purpose

The present study investigated the effects of high- versus low-quality protein supplementation on the regain of exercise performance during recovery from a period of high-intensity resistance training.

Methods

In a diet-controlled crossover study, 12 resistance-trained participants performed two identical training periods, with each training period including four sessions of high-intensity resistance exercise during 5 days, while receiving either high- or low-quality protein. Prior to and at 3, 24 and 48 h after the training periods, performance was evaluated in knee extensor and flexor isometric maximal voluntary contraction (MVC), counter-movement jumping height (CMJ), and peak and mean anaerobic power. In addition, prior to and at 48 h after the training periods, performance in time-to-exhaustion at 70 % of VO2max (TTE) was evaluated.

Results

After the intense training periods, decrements in the order of 4–24 % were observed for MVCext, CMJ, mean anaerobic power, and TTE. In particular for TTE, this decrement in exercise performance did not attain full recovery at 48 h post-exercise. The regain of exercise performance was not dictated by type of protein supplement.

Conclusion

The regain of muscle strength as well as anaerobic or aerobic performances were not markedly influenced by the type of protein supplement.
Literature
go back to reference Anthony JC, Anthony TG, Kimball SR, Vary TC, Jefferson LS (2000) Orally administered leucine stimulates protein synthesis in skeletal muscle of postabsorptive rats in association with increased eIF4F formation. J Nutr 130(2):139–145PubMed Anthony JC, Anthony TG, Kimball SR, Vary TC, Jefferson LS (2000) Orally administered leucine stimulates protein synthesis in skeletal muscle of postabsorptive rats in association with increased eIF4F formation. J Nutr 130(2):139–145PubMed
go back to reference Asp S, Kristiansen S, Richter EA (1995) Eccentric muscle damage transiently decreases rat skeletal muscle GLUT-4 protein. J Appl Physiol (1985) 79(4):1338–1345 Asp S, Kristiansen S, Richter EA (1995) Eccentric muscle damage transiently decreases rat skeletal muscle GLUT-4 protein. J Appl Physiol (1985) 79(4):1338–1345
go back to reference Asp S, Daugaard JR, Kristiansen S, Kiens B, Richter EA (1996) Eccentric exercise decreases maximal insulin action in humans: muscle and systemic effects. J Physiol 494(Pt 3):891–898CrossRefPubMedPubMedCentral Asp S, Daugaard JR, Kristiansen S, Kiens B, Richter EA (1996) Eccentric exercise decreases maximal insulin action in humans: muscle and systemic effects. J Physiol 494(Pt 3):891–898CrossRefPubMedPubMedCentral
go back to reference Bijur PE, Silver W, Gallagher EJ (2001) Reliability of the visual analog scale for measurement of acute pain. Acad Emerg Med 8(12):1153–1157CrossRefPubMed Bijur PE, Silver W, Gallagher EJ (2001) Reliability of the visual analog scale for measurement of acute pain. Acad Emerg Med 8(12):1153–1157CrossRefPubMed
go back to reference Biolo G, Tipton KD, Klein S, Wolfe RR (1997) An abundant supply of amino acids enhances the metabolic effect of exercise on muscle protein. Am J Physiol 273(1 Pt 1):E122–E129PubMed Biolo G, Tipton KD, Klein S, Wolfe RR (1997) An abundant supply of amino acids enhances the metabolic effect of exercise on muscle protein. Am J Physiol 273(1 Pt 1):E122–E129PubMed
go back to reference Brzycki M (1993) Strength testing—predicting a one-rep max from a reps-to-fatigue. J Phys Educ Recreat Dance 64(1):88–90CrossRef Brzycki M (1993) Strength testing—predicting a one-rep max from a reps-to-fatigue. J Phys Educ Recreat Dance 64(1):88–90CrossRef
go back to reference Buckley JD, Thomson RL, Coates AM, Howe PR, DeNichilo MO, Rowney MK (2010) Supplementation with a whey protein hydrolysate enhances recovery of muscle force-generating capacity following eccentric exercise. J Sci Med Sport 13(1):178–181CrossRefPubMed Buckley JD, Thomson RL, Coates AM, Howe PR, DeNichilo MO, Rowney MK (2010) Supplementation with a whey protein hydrolysate enhances recovery of muscle force-generating capacity following eccentric exercise. J Sci Med Sport 13(1):178–181CrossRefPubMed
go back to reference Churchward-Venne TA, Burd NA, Mitchell CJ, West DW, Philp A, Marcotte GR, Baker SK, Baar K, Phillips SM (2012) Supplementation of a suboptimal protein dose with leucine or essential amino acids: effects on myofibrillar protein synthesis at rest and following resistance exercise in men. J Physiol. doi:10.1113/jphysiol.2012.228833 Churchward-Venne TA, Burd NA, Mitchell CJ, West DW, Philp A, Marcotte GR, Baker SK, Baar K, Phillips SM (2012) Supplementation of a suboptimal protein dose with leucine or essential amino acids: effects on myofibrillar protein synthesis at rest and following resistance exercise in men. J Physiol. doi:10.​1113/​jphysiol.​2012.​228833
go back to reference Churchward-Venne TA, Breen L, Di Donato DM, Hector AJ, Mitchell CJ, Moore DR, Stellingwerff T, Breuille D, Offord EA, Baker SK, Phillips SM (2014) Leucine supplementation of a low-protein mixed macronutrient beverage enhances myofibrillar protein synthesis in young men: a double-blind, randomized trial. Am J Clin Nutr 99(2):276–286. doi:10.3945/ajcn.113.068775 CrossRefPubMed Churchward-Venne TA, Breen L, Di Donato DM, Hector AJ, Mitchell CJ, Moore DR, Stellingwerff T, Breuille D, Offord EA, Baker SK, Phillips SM (2014) Leucine supplementation of a low-protein mixed macronutrient beverage enhances myofibrillar protein synthesis in young men: a double-blind, randomized trial. Am J Clin Nutr 99(2):276–286. doi:10.​3945/​ajcn.​113.​068775 CrossRefPubMed
go back to reference Farup J, Rahbek SK, Knudsen IS, de Paoli F, Mackey AL, Vissing K (2014) Whey protein supplementation accelerates satellite cell proliferation during recovery from eccentric exercise. Amino Acids 46(11):2503–2516. doi:10.1007/s00726-014-1810-3 CrossRefPubMed Farup J, Rahbek SK, Knudsen IS, de Paoli F, Mackey AL, Vissing K (2014) Whey protein supplementation accelerates satellite cell proliferation during recovery from eccentric exercise. Amino Acids 46(11):2503–2516. doi:10.​1007/​s00726-014-1810-3 CrossRefPubMed
go back to reference Farup J, Rahbek SK, Bjerre J, de Paoli F, Vissing K (2015) Associated decrements in rate of force development and neural drive after maximal eccentric exercise. Scand J Med Sci Sports. doi:10.1111/sms.12481 (Accepted) Farup J, Rahbek SK, Bjerre J, de Paoli F, Vissing K (2015) Associated decrements in rate of force development and neural drive after maximal eccentric exercise. Scand J Med Sci Sports. doi:10.​1111/​sms.​12481 (Accepted)
go back to reference Green MS, Doyle JA, Ingalls CP, Benardot D, Rupp JC, Corona BT (2010) Adaptation of insulin-resistance indicators to a repeated bout of eccentric exercise in human skeletal muscle. Int J Sport Nutr Exerc Metab 20(3):181–190CrossRefPubMed Green MS, Doyle JA, Ingalls CP, Benardot D, Rupp JC, Corona BT (2010) Adaptation of insulin-resistance indicators to a repeated bout of eccentric exercise in human skeletal muscle. Int J Sport Nutr Exerc Metab 20(3):181–190CrossRefPubMed
go back to reference Hartman JW, Tang JE, Wilkinson SB, Tarnopolsky MA, Lawrence RL, Fullerton AV, Phillips SM (2007) Consumption of fat-free fluid milk after resistance exercise promotes greater lean mass accretion than does consumption of soy or carbohydrate in young, novice, male weightlifters. Am J Clin Nutr 86(2):373–381PubMed Hartman JW, Tang JE, Wilkinson SB, Tarnopolsky MA, Lawrence RL, Fullerton AV, Phillips SM (2007) Consumption of fat-free fluid milk after resistance exercise promotes greater lean mass accretion than does consumption of soy or carbohydrate in young, novice, male weightlifters. Am J Clin Nutr 86(2):373–381PubMed
go back to reference Hoffman JR, Ratamess NA, Tranchina CP, Rashti SL, Kang J, Faigenbaum AD (2010) Effect of a proprietary protein supplement on recovery indices following resistance exercise in strength/power athletes. Amino Acids 38(3):771–778. doi:10.1007/s00726-009-0283-2 CrossRefPubMed Hoffman JR, Ratamess NA, Tranchina CP, Rashti SL, Kang J, Faigenbaum AD (2010) Effect of a proprietary protein supplement on recovery indices following resistance exercise in strength/power athletes. Amino Acids 38(3):771–778. doi:10.​1007/​s00726-009-0283-2 CrossRefPubMed
go back to reference Hulmi JJ, Lockwood CM, Stout JR (2010) Effect of protein/essential amino acids and resistance training on skeletal muscle hypertrophy: a case for whey protein. Nutr Metab (Lond) 7:51. doi:10.1186/1743-7075-7-51 CrossRef Hulmi JJ, Lockwood CM, Stout JR (2010) Effect of protein/essential amino acids and resistance training on skeletal muscle hypertrophy: a case for whey protein. Nutr Metab (Lond) 7:51. doi:10.​1186/​1743-7075-7-51 CrossRef
go back to reference Jackman SR, Witard OC, Jeukendrup AE, Tipton KD (2010) Branched-chain amino acid ingestion can ameliorate soreness from eccentric exercise. Med Sci Sports Exerc 42(5):962–970CrossRefPubMed Jackman SR, Witard OC, Jeukendrup AE, Tipton KD (2010) Branched-chain amino acid ingestion can ameliorate soreness from eccentric exercise. Med Sci Sports Exerc 42(5):962–970CrossRefPubMed
go back to reference Kirby TJ, Triplett NT, Haines TL, Skinner JW, Fairbrother KR, McBride JM (2012) Effect of leucine supplementation on indices of muscle damage following drop jumps and resistance exercise. Amino Acids 42(5):1987–1996. doi:10.1007/s00726-011-0928-9 CrossRefPubMed Kirby TJ, Triplett NT, Haines TL, Skinner JW, Fairbrother KR, McBride JM (2012) Effect of leucine supplementation on indices of muscle damage following drop jumps and resistance exercise. Amino Acids 42(5):1987–1996. doi:10.​1007/​s00726-011-0928-9 CrossRefPubMed
go back to reference Kraemer WJ, Hooper DR, Szivak TK, Kupchak BR, Dunn-Lewis C, Comstock BA, Flanagan SD, Looney DP, Sterczala AJ, DuPont WH, Pryor JL, Luk HY, Maladoungdock J, McDermott D, Volek JS, Maresh CM (2015) The addition of beta-hydroxy-beta-methylbutyrate and isomaltulose to whey protein improves recovery from highly demanding resistance exercise. J Am Coll Nutr 34(2):91–99. doi:10.1080/07315724.2014.938790 CrossRefPubMed Kraemer WJ, Hooper DR, Szivak TK, Kupchak BR, Dunn-Lewis C, Comstock BA, Flanagan SD, Looney DP, Sterczala AJ, DuPont WH, Pryor JL, Luk HY, Maladoungdock J, McDermott D, Volek JS, Maresh CM (2015) The addition of beta-hydroxy-beta-methylbutyrate and isomaltulose to whey protein improves recovery from highly demanding resistance exercise. J Am Coll Nutr 34(2):91–99. doi:10.​1080/​07315724.​2014.​938790 CrossRefPubMed
go back to reference Michailidis Y, Karagounis LG, Terzis G, Jamurtas AZ, Spengos K, Tsoukas D, Chatzinikolaou A, Mandalidis D, Stefanetti RJ, Papassotiriou I, Athanasopoulos S, Hawley JA, Russell AP, Fatouros IG (2013) Thiol-based antioxidant supplementation alters human skeletal muscle signaling and attenuates its inflammatory response and recovery after intense eccentric exercise. Am J Clin Nutr 98(1):233–245. doi:10.3945/ajcn.112.049163 CrossRefPubMed Michailidis Y, Karagounis LG, Terzis G, Jamurtas AZ, Spengos K, Tsoukas D, Chatzinikolaou A, Mandalidis D, Stefanetti RJ, Papassotiriou I, Athanasopoulos S, Hawley JA, Russell AP, Fatouros IG (2013) Thiol-based antioxidant supplementation alters human skeletal muscle signaling and attenuates its inflammatory response and recovery after intense eccentric exercise. Am J Clin Nutr 98(1):233–245. doi:10.​3945/​ajcn.​112.​049163 CrossRefPubMed
go back to reference Moberg M, Apro W, Ohlsson I, Ponten M, Villanueva A, Ekblom B, Blomstrand E (2014) Absence of leucine in an essential amino acid supplement reduces activation of mTORC1 signalling following resistance exercise in young females. Appl Physiol Nutr Metab 39(2):183–194. doi:10.1139/apnm-2013-0244 CrossRefPubMed Moberg M, Apro W, Ohlsson I, Ponten M, Villanueva A, Ekblom B, Blomstrand E (2014) Absence of leucine in an essential amino acid supplement reduces activation of mTORC1 signalling following resistance exercise in young females. Appl Physiol Nutr Metab 39(2):183–194. doi:10.​1139/​apnm-2013-0244 CrossRefPubMed
go back to reference Nelson AR, Phillips SM, Stellingwerff T, Rezzi S, Bruce SJ, Breton I, Thorimbert A, Guy PA, Clarke J, Broadbent S, Rowlands DS (2012) A protein-leucine supplement increases branched-chain amino acid and nitrogen turnover but not performance. Med Sci Sports Exerc 44(1):57–68. doi:10.1249/MSS.0b013e3182290371 CrossRefPubMed Nelson AR, Phillips SM, Stellingwerff T, Rezzi S, Bruce SJ, Breton I, Thorimbert A, Guy PA, Clarke J, Broadbent S, Rowlands DS (2012) A protein-leucine supplement increases branched-chain amino acid and nitrogen turnover but not performance. Med Sci Sports Exerc 44(1):57–68. doi:10.​1249/​MSS.​0b013e3182290371​ CrossRefPubMed
go back to reference Norden (2012) Nordic nutrition recommendations 2012: integrating nutrition and physical activity. Nordic Council of Ministers, Copenhagen K Norden (2012) Nordic nutrition recommendations 2012: integrating nutrition and physical activity. Nordic Council of Ministers, Copenhagen K
go back to reference Pasiakos SM, Lieberman HR, McLellan TM (2014) Effects of protein supplements on muscle damage, soreness and recovery of muscle function and physical performance: a systematic review. Sports Med. doi:10.1007/s40279-013-0137-7 (Epub ahead of print) Pasiakos SM, Lieberman HR, McLellan TM (2014) Effects of protein supplements on muscle damage, soreness and recovery of muscle function and physical performance: a systematic review. Sports Med. doi:10.​1007/​s40279-013-0137-7 (Epub ahead of print)
go back to reference Proske U, Allen TJ (2005) Damage to skeletal muscle from eccentric exercise. Exerc Sport Sci Rev 33(2):98–104CrossRefPubMed Proske U, Allen TJ (2005) Damage to skeletal muscle from eccentric exercise. Exerc Sport Sci Rev 33(2):98–104CrossRefPubMed
go back to reference Ratamess NA, Kraemer WJ, Volek JS, Rubin MR, Gomez AL, French DN, Sharman MJ, McGuigan MM, Scheett T, Hakkinen K, Newton RU, Dioguardi F (2003) The effects of amino acid supplementation on muscular performance during resistance training overreaching. J Strength Cond Res 17(2):250–258PubMed Ratamess NA, Kraemer WJ, Volek JS, Rubin MR, Gomez AL, French DN, Sharman MJ, McGuigan MM, Scheett T, Hakkinen K, Newton RU, Dioguardi F (2003) The effects of amino acid supplementation on muscular performance during resistance training overreaching. J Strength Cond Res 17(2):250–258PubMed
go back to reference Skillen RA, Testa M, Applegate EA, Heiden EA, Fascetti AJ, Casazza GA (2008) Effects of an amino acid carbohydrate drink on exercise performance after consecutive-day exercise bouts. Int J Sport Nutr Exerc Metab 18(5):473–492CrossRefPubMed Skillen RA, Testa M, Applegate EA, Heiden EA, Fascetti AJ, Casazza GA (2008) Effects of an amino acid carbohydrate drink on exercise performance after consecutive-day exercise bouts. Int J Sport Nutr Exerc Metab 18(5):473–492CrossRefPubMed
go back to reference Thomson JS, Ali A, Rowlands DS (2011) Leucine-protein supplemented recovery feeding enhances subsequent cycling performance in well-trained men. Appl Physiol Nutr Metab 36(2):242–253. doi:10.1139/h10-104 CrossRefPubMed Thomson JS, Ali A, Rowlands DS (2011) Leucine-protein supplemented recovery feeding enhances subsequent cycling performance in well-trained men. Appl Physiol Nutr Metab 36(2):242–253. doi:10.​1139/​h10-104 CrossRefPubMed
go back to reference Tipton KD, Ferrando AA, Phillips SM, Doyle D Jr, Wolfe RR (1999) Postexercise net protein synthesis in human muscle from orally administered amino acids. Am J Physiol 276(4 Pt 1):E628–E634PubMed Tipton KD, Ferrando AA, Phillips SM, Doyle D Jr, Wolfe RR (1999) Postexercise net protein synthesis in human muscle from orally administered amino acids. Am J Physiol 276(4 Pt 1):E628–E634PubMed
go back to reference Volek JS, Volk BM, Gomez AL, Kunces LJ, Kupchak BR, Freidenreich DJ, Aristizabal JC, Saenz C, Dunn-Lewis C, Ballard KD, Quann EE, Kawiecki DL, Flanagan SD, Comstock BA, Fragala MS, Earp JE, Fernandez ML, Bruno RS, Ptolemy AS, Kellogg MD, Maresh CM, Kraemer WJ (2013) Whey protein supplementation during resistance training augments lean body mass. J Am Coll Nutr 32(2):122–135. doi:10.1080/07315724.2013.793580 CrossRefPubMed Volek JS, Volk BM, Gomez AL, Kunces LJ, Kupchak BR, Freidenreich DJ, Aristizabal JC, Saenz C, Dunn-Lewis C, Ballard KD, Quann EE, Kawiecki DL, Flanagan SD, Comstock BA, Fragala MS, Earp JE, Fernandez ML, Bruno RS, Ptolemy AS, Kellogg MD, Maresh CM, Kraemer WJ (2013) Whey protein supplementation during resistance training augments lean body mass. J Am Coll Nutr 32(2):122–135. doi:10.​1080/​07315724.​2013.​793580 CrossRefPubMed
go back to reference Wilkinson SB, Tarnopolsky MA, Macdonald MJ, Macdonald JR, Armstrong D, Phillips SM (2007) Consumption of fluid skim milk promotes greater muscle protein accretion after resistance exercise than does consumption of an isonitrogenous and isoenergetic soy-protein beverage. Am J Clin Nutr 85(4):1031–1040PubMed Wilkinson SB, Tarnopolsky MA, Macdonald MJ, Macdonald JR, Armstrong D, Phillips SM (2007) Consumption of fluid skim milk promotes greater muscle protein accretion after resistance exercise than does consumption of an isonitrogenous and isoenergetic soy-protein beverage. Am J Clin Nutr 85(4):1031–1040PubMed
go back to reference Witard OC, Turner JE, Jackman SR, Kies AK, Jeukendrup AE, Bosch JA, Tipton KD (2014) High dietary protein restores overreaching induced impairments in leukocyte trafficking and reduces the incidence of upper respiratory tract infection in elite cyclists. Brain Behav Immun 39:211–219. doi:10.1016/j.bbi.2013.10.002 CrossRefPubMed Witard OC, Turner JE, Jackman SR, Kies AK, Jeukendrup AE, Bosch JA, Tipton KD (2014) High dietary protein restores overreaching induced impairments in leukocyte trafficking and reduces the incidence of upper respiratory tract infection in elite cyclists. Brain Behav Immun 39:211–219. doi:10.​1016/​j.​bbi.​2013.​10.​002 CrossRefPubMed
go back to reference Wolfe RR (2006) Skeletal muscle protein metabolism and resistance exercise. J Nutr 136(2):525S–528SPubMed Wolfe RR (2006) Skeletal muscle protein metabolism and resistance exercise. J Nutr 136(2):525S–528SPubMed
Metadata
Title
Effect of protein quality on recovery after intense resistance training
Authors
E. Rindom
M. H. Nielsen
K. Kececi
M. E. Jensen
K. Vissing
J. Farup
Publication date
01-12-2016
Publisher
Springer Berlin Heidelberg
Published in
European Journal of Applied Physiology / Issue 11-12/2016
Print ISSN: 1439-6319
Electronic ISSN: 1439-6327
DOI
https://doi.org/10.1007/s00421-016-3477-9

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