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Indian Journal of Hematology and Blood Transfusion
Published in: Indian Journal of Hematology and Blood Transfusion 3/2018

01-07-2018 | Original Article

Effect of Nitric Oxide Donor on Metabolism of Apheresis Platelets

Authors: Lu Yu, Shifang Yu, Yunlei He, Qiming Li, Deyi Xu, Kai Huang, Gang Deng, Qiang Li

Published in: Indian Journal of Hematology and Blood Transfusion | Issue 3/2018

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Abstract

The aim of this study is to investigate the effects of a nitric oxide (NO) donor S-nitrosoglutathione (GSNO) on the metabolism and improvement of preservation quality in apheresis platelets. A GSNO solution with a certain concentration was added into fresh apheresis platelets, and the parameters associated with platelet morphology, metabolism and function were temporally monitored for 7 days. The results showed that the NO level in GSNO group were remarkably higher than those in the control group during the whole storage stage. No significant morphology or function difference was observed between the control and GSNO groups such as Platelet count, platelet distribution width, mean platelet volume and mitochondrial metabolic activity. But in metabolism there are something differences from morphology data: pCO2, pO2, cHCO3 − were also found to have no clear difference between the control and GSNO groups; the lactic acid content, sugar consumption and Lactate dehydrogenase activity in the GSNO group were lower than that in the control group at some time point; and pH values in the GSNO group were higher than the control group. Our study discovered that the NO donor GSNO can reduce the metabolism and maintain the cellular characteristics of platelets in vitro during the platelet storage period.
Literature
1.
2.
Thon JN, Schubert P, Devine DV (2008) Platelet storage lesion: a new understanding from a proteomic perspective. Transfus Med Rev 22(4):268–279CrossRefPubMed
3.
4.
Zhang JG, Carter CJ, Culibrk B, Devine DV, Levin E, Scammell K, Weiss S, Gyongyossy-Issa MI (2008) Buffy-coat platelet variables and metabolism during storage in additive solutions or plasma. Transfusion 48(5):847–856CrossRefPubMed
5.
Li J, Xia Y, Bertino AM, Coburn JP, Kuter DJ (2000) The mechanism of apoptosis in human platelets during storage. Transfusion 40(11):1320–1329CrossRefPubMed
6.
Solheim BG, Flesland O, Seghatchian J, Brosstad F (2004) Clinical implications of red blood cell and platelet storage lesions: an overview. Transfus Apher Sci 31(3):185–189CrossRefPubMed
7.
Tulika C, Ashish G, Ashutosh K, Sheeba A (2011) Morphological and functional changes in random donor platelets stored for seven days in platelet additive solution. Int J Blood Transfus Immunohematol 1:20CrossRef
8.
van der Meer PF, de Korte D (2011) Platelet preservation: agitation and containers. Transfus Apher Sci 44(3):297–304CrossRefPubMed
9.
Sandgren P, Saeed K (2011) Storage of buffy-coat-derived platelets in additive solution: in vitro effects on platelets of the air bubbles and foam included in the final unit. Blood Transfus 9(2):182–188PubMedPubMedCentral
10.
Schubert P, Devine DV (2010) Towards targeting platelet storage lesion-related signaling pathways. Blood Transfus 8(Suppl 3):s69–s72PubMedPubMedCentral
11.
Dumont LJ, AuBuchon JP, Gulliksson H, Slichter SJ, Elfath MD, Holme S, Murphy JR, Rose LE, Popovsky MA, Murphy S (2006) In vitro pH effects on in vivo recovery and survival of platelets: an analysis by the BEST collaborative. Transfusion 46(8):1300–1305CrossRefPubMed
12.
Shapira S, Friedman Z, Shapiro H, Presseizen K, Radnay J, Ellis MH (2000) The effect of storage on the expression of platelet membrane phosphatidylserine and the subsequent impacton the coagulant function of stored platelets. Transfusion 40(10):1257–1263CrossRefPubMed
13.
Seghatchian J, Krailadsiri P (2001) Platelet storage lesion and apoptosis: are they related? Transfus Apher Sci 24(1):103–105CrossRefPubMed
14.
Wood EM, Colton E, Yomtovian RA, Currie LM, Connor J, Anderson JM (2000) Prevention of monocyte adhesion and inflammatory cytokine production during blood platelet storage: an in vitro model with implications for transfusion practice. J Biomed Mater Res 51(2):147–154CrossRefPubMed
15.
16.
Sener A, Egemen G, Cevik O, Yanikkaya-Demirel G, Apikoglu-Rabus S, Ozsavci D (2013) In vitro effects of nitric oxide donors on apoptosis and oxidative/nitrative protein modifications in ADP-activated platelets. Hum Exp Toxicol 32(3):225–235CrossRefPubMed
17.
Riss TL, Moravec RA (1992) Comparison of MTT, XTT, and a novel tetrazolium compound MTS for in vitro proliferation and chemosensitivity assays. Mol Biol Cell 3:184–194
18.
Xiong J, Zhu J, Dai W, Dong C, Qiu Q, Li C (2017) Integrating gut microbiota immaturity and disease-discriminatory taxa to diagnose the initiation and severity of shrimp disease. Environ Microbiol 19(4):1490–1501CrossRefPubMed
19.
Dai W, Zhang J, Tu Q, Deng Y, Qiu Q, Xiong J (2017) Bacterioplankton assembly and interspecies interaction indicating increasing coastal eutrophication. Chemosphere 177:317–325CrossRefPubMed
20.
Xiong J, Zhu J, Wang K, Wang X, Ye X, Liu L, Zhao Q, Hou M, Qiuqian L, Zhang D (2014) The temporal scaling of bacterioplankton composition: high turnover and predictability during shrimp cultivation. Microb Ecol 67(2):256–264CrossRefPubMed
21.
Cesar J, DiMinno G, Alam I, Silver M, Murphy S (1987) Plasma free fatty acid metabolism during storage of platelet concentrates for transfusion. Transfusion 27(5):434–437CrossRefPubMed
22.
Gulliksson H (2003) Defining the optimal storage conditions for the long-term storage of platelets. Transfus Med Rev 17(3):209–215CrossRefPubMed
23.
Gkaliagkousi E, Ritter J, Ferro A (2007) Platelet-derived nitric oxide signaling and regulation. Circ Res 101(7):654–662CrossRefPubMed
24.
Marjanovic JA, Stojanovic A, Brovkovych VM, Skidgel RA, Du X (2008) Signaling-mediated functional activation of inducible nitric-oxide synthase and its role in stimulating platelet activation. J Biol Chem 283(43):28827–28834CrossRefPubMedPubMedCentral
25.
Oberprieler NG, Roberts W, Riba R, Graham AM, Homer-Vanniasinkam S, Naseem KM (2007) cGMP-independent inhibition of integrin alphaIIbbeta3-mediated platelet adhesion and outside-in signalling by nitric oxide. FEBS Lett 581(7):1529–1534CrossRefPubMed
26.
Shiri R, Yari F, Ahmadinejad M, Vaeli S, Tabatabaei MR (2014) The caspase-3 inhibitor (peptide Z-DEVD-FMK) affects the survival and function of platelets in platelet concentrate during storage. Blood research 49(1):49–53CrossRefPubMedPubMedCentral
27.
Picker SM, Schneider V, Oustianskaia L, Gathof BS (2009) Cell viability during platelet storage in correlation to cellular metabolism after different pathogen reduction technologies. Transfusion 49(11):2311–2318CrossRefPubMed
28.
Tynngard N, Wallstedt M, Sodergren AL, Faxalv L, Ramstrom S (2015) Platelet adhesion changes during storage studied with a novel method using flow cytometry and protein-coated beads. Platelets 26(2):177–185CrossRefPubMed
29.
Deyhim MR, Mesbah-Namin SA, Yari F, Taghikhani M, Amirizadeh N (2015) L-carnitine effectively improves the metabolism and quality of platelet concentrates during storage. Ann Hematol 94(4):671–680CrossRefPubMed
30.
Tynngard N (2009) Preparation, storage and quality control of platelet concentrates. Transfus Apher Sci 41(2):97–104CrossRefPubMed
31.
Crane MS, Ollosson R, Moore KP, Rossi AG, Megson IL (2002) Novel role for low molecular weight plasma thiols in nitric oxide-mediated control of platelet function. J Biol Chem 277(49):46858–46863CrossRefPubMed
32.
Skrzypchak AM, Lafayette NG, Bartlett RH, Zhou Z, Frost MC, Meyerhoff ME, Reynolds MM, Annich GM (2007) Effect of varying nitric oxide release to prevent platelet consumption and preserve platelet function in an in vivo model of extracorporeal circulation. Perfusion 22(3):193–200CrossRefPubMed
33.
Wu T, Zhang C, Wang Z, Liu J, Li H, Zhou W, Wang S (2013) Inhibitory role of S-nitrosoglutathione in the aggregation of frozen platelets, and its effect on the expression of membrane glycoproteins. Exp Ther Med 6(3):831–839CrossRefPubMedPubMedCentral
34.
Lee JH, Kim JT, Cho YG (2008) Effect of nitric oxide on the cryopreservation of platelets. Korean J Lab Med 28(2):136–143CrossRefPubMed
35.
Wong K, Li X (2004) Nitric oxide infusion alleviates cellular activation during preparation, leukofiltration and storage of platelets. Transfus Apher Sci 30(1):29–39CrossRefPubMed
Metadata
Title
Effect of Nitric Oxide Donor on Metabolism of Apheresis Platelets
Authors
Lu Yu
Shifang Yu
Yunlei He
Qiming Li
Deyi Xu
Kai Huang
Gang Deng
Qiang Li
Publication date
01-07-2018
Publisher
Springer India
Published in
Indian Journal of Hematology and Blood Transfusion / Issue 3/2018
Print ISSN: 0971-4502
Electronic ISSN: 0974-0449
DOI
https://doi.org/10.1007/s12288-017-0881-1

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