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01-01-2010 | Original Article

Effect of low-level laser irradiation and epidermal growth factor on adult human adipose-derived stem cells

Authors: B. Mvula, T. J. Moore, H. Abrahamse

Published in: Lasers in Medical Science | Issue 1/2010

Abstract

The study investigated the effects of low-level laser radiation and epidermal growth factor (EGF) on adult adipose-derived stem cells (ADSCs) isolated from human adipose tissue. Isolated cells were cultured to semi-confluence, and the monolayers of ADSCs were exposed to low-level laser at 5 J/cm² using 636 nm diode laser. Cell viability and proliferation were monitored using adenosine triphosphate (ATP) luminescence and optical density at 0 h, 24 h and 48 h after irradiation. Application of low-level laser irradiation at 5 J/cm² on human ADSCs cultured with EGF increased the viability and proliferation of these cells. The results indicate that low-level laser irradiation in combination with EGF enhances the proliferation and maintenance of ADSCs in vitro.

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Title: Effect of low-level laser irradiation and epidermal growth factor on adult human adipose-derived stem cells
Authors: B. Mvula
T. J. Moore
H. Abrahamse
Publication date: 01-01-2010
Publisher: Springer-Verlag
Published in: Lasers in Medical Science / Issue 1/2010
Print ISSN: 0268-8921
Electronic ISSN: 1435-604X
DOI: https://doi.org/10.1007/s10103-008-0636-1

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Effect of low-level laser irradiation and epidermal growth factor on adult human adipose-derived stem cells

Abstract

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