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Open Access 01-12-2011 | Methodology

Effect of liver growth factor on both testicular regeneration and recovery of spermatogenesis in busulfan-treated mice

Authors: Miriam Pérez-Crespo, Eva Pericuesta, Serafín Pérez-Cerezales, Maria I Arenas, Maria VT Lobo, Juan J Díaz-Gil, Alfonso Gutierrez-Adan

Published in: Reproductive Biology and Endocrinology | Issue 1/2011

Abstract

Background

Some adult stem cells persist in adult tissue; however, we do not know how to stimulate stem cells in adults to heal injuries. Liver growth factor (LGF) is a biliprotein with hepatic mitogen activity. Its concentration increases markedly in the presence of any type of liver injury, and it shows in vivo therapeutic biological activity at extrahepatic sites.

Methods

We have analyzed the effect of LGF on the replenishment of germinal cells in the testes of mice injected with busulfan, a common cancer drug that also specifically affects germ line stem cells and spermatogonia. We determined the testicular and epididymal weight, spermatozoal concentration in the epididymis and sperm motility, and performed a histological analysis.

Results

Intraperitoneal administration of LGF was able to partially restore spermatogenesis, as well as sperm production and motility, in mice sterilized with busulfan. LGF treatment in busulfan-treated animals that have suffered a disruption of spermatogenesis can accelerate the reactivation of this process in most of the tubules, as shown in the histological analysis.

Conclusions

Our results suggest a potential use of LGF in the mobilization of testicular stem cells and in the restoration of spermatogenesis after busulfan-induced damage to the testicular germinal epithelium.

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Title: Effect of liver growth factor on both testicular regeneration and recovery of spermatogenesis in busulfan-treated mice
Authors: Miriam Pérez-Crespo
Eva Pericuesta
Serafín Pérez-Cerezales
Maria I Arenas
Maria VT Lobo
Juan J Díaz-Gil
Alfonso Gutierrez-Adan
Publication date: 01-12-2011
Publisher: BioMed Central
Published in: Reproductive Biology and Endocrinology / Issue 1/2011
Electronic ISSN: 1477-7827
DOI: https://doi.org/10.1186/1477-7827-9-21

Keynote webinar | Spotlight on medication adherence

Springer Medicine

Effect of liver growth factor on both testicular regeneration and recovery of spermatogenesis in busulfan-treated mice

Abstract

Background

Methods

Results

Conclusions

Keynote webinar | Spotlight on medication adherence

Springer Medicine

Abstract

Background

Methods

Results

Conclusions

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