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BMC Oral Health
Published in: BMC Oral Health 1/2023

Open Access 01-12-2023 | Research

Effect of lactoferrin on enamel characteristics of primary and permanent teeth: an in-vitro study

Authors: Nouran Samir Mohamed Atia, Reham Ahmed El-nemr, Asmaa Ali Emam Abo-Elsoud

Published in: BMC Oral Health | Issue 1/2023

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Abstract

Background

Lactoferrin, a glycoprotein naturally found in breast milk, is known for its bactericidal and antiviral properties, as well as its capacity to modulate the immune system; therefore, pediatricians routinely recommend it as dietary support. The objective of this study was to determine how lactoferrin oral suspension could affect the enamel surface characteristics of primary and permanent teeth.

Methods

This research was conducted on 40 unidentified extracted teeth, including primary and permanent teeth. Experimental teeth were free of cracks or enamel defects, as confirmed by careful examination using a dental operating microscope. The crowns were bisected into 80 specimens and assorted into two groups based on the type of dentition. Group DM included 40 specimens of second deciduous molars, while Group PM contained 40 samples of first premolars. Each of the DM and PM specimens was subsequently split based on the type of dispersion medium into two subgroups: a control subgroup (artificial saliva) and a test subgroup (lactoferrin suspension). The specimens were immersed in lactoferrin suspension for two minutes, then kept in artificial saliva for the rest of the 24 h for 30 successive days. This is a pioneering study about the effect of orally supplemented lactoferrin on teeth; therefore, we examined enamel hardness, ultra-morphology, and mineral contents.

Results

Our findings indicated a highly significant decrease (p < 0.01) in the microhardness of the lactoferrin subgroup in Group DM (second deciduous molars) and a significant reduction (p < 0.05) in the microhardness of the lactoferrin subgroup in Group PM (premolars). Calcium weight% was not statistically different (p > 0.05) compared with a significant decline (p < 0.05) in phosphorus weight% in lactoferrin subgroups in both DM and PM groups. The enamel surface of lactoferrin subgroups in both DM and PM groups was demineralized and porous, with the enamel of deciduous teeth being more affected by lactoferrin than permanent teeth.

Conclusion

Lactoferrin suspension decreased the microhardness of enamel and both calcium and phosphorus weight percentages. Both dentitions exhibited erosions in the enamel surface, with primary teeth being more affected than the permanent teeth.
Literature
1.
2.
Ortiz Y, García-Amézquita E, Acosta CH, Sepúlveda DR. Functional dairy products. Global food Security and Wellness. 2017:67–103.
3.
Takayama Y, Aoki R. Roles of lactoferrin on skin wound healing. Biochem Cell Biol. 2012;90:497–503.CrossRefPubMed
4.
5.
El-Khawaga A, Abdelmaksoud H. Effect of lactoferrin supplementation on iron Deficiency anemia in primary school children. IJMA. 2019;1:48–52.
6.
Bahal P, Djemal S. Dental erosion from an excess of vitamin C. Case Rep Dent. 2014; 2014: 485387.
7.
Mukundan D, Vignesh R, Ravindran V. Comparative evaluation on the effects of three pediatric syrups on microhardness, roughness and staining of the primary teeth enamel: an in-vitro study. Cureus. 2023;15:e42764.PubMedPubMedCentral
8.
Scatena C, Galafassi D, Gomes-Silva JM, Borsatto MC, Serra MC. In vitro erosive effect of pediatric medicines on deciduous tooth enamel. Braz Dent J. 2014;25:22–7.CrossRefPubMed
9.
Hunter ML, West NX, Hughes JA, Newcombe RG, Addy M. Erosion of deciduous and permanent dental hard tissue in the oral environment. J Dent. 2000;28:257–63.CrossRefPubMed
10.
Wang LJ, Tang R, Bonstein T, Bush P, Nancollas GH. Enamel demineralization in primary and permanent teeth. J Dent Res. 2006;85:359–63.CrossRefPubMed
11.
Alex V, Abraham K, Khosla E, James AR, Thenumkal E. Pediatric syrups: a risk to enamel microhardness??? Ann Essences dent. 2017;9:5–9.
12.
Faul F, Erdfelder E, Lang AG, Buchner A. G* power 3: a flexible statistical power analysis program for the social, behavioral, and biomedical sciences. Behav Res Methods. 2007;39:175–91.CrossRefPubMed
13.
Guler EB, Bayrak GD, Unsal M, Kuvvetli SS. Effect of pediatric multivitamin syrups and effervescent tablets on the surface microhardness and roughness of restorative materials: an in vitro study. J Dent Sci. 2021;16:311–7.CrossRef
14.
Gupta M, Panda S. Cariogenic potential of the commonly prescribed pediatric liquid medicaments in Kingdom of Saudi Arabia: an in vitro study. J Contemp Dent Pract. 2017;18:307–11.CrossRefPubMed
15.
Habold C, Dunel-Erb S, Chevalier C, Laurent P, Le Maho Y, Lignot JH. Observations of the intestinal mucosa using environmental scanning electron microscopy (ESEM); comparison with conventional scanning electron microscopy (CSEM). Micron. 2003;34:373–9.CrossRefPubMed
16.
Manzoni P, Dall’Agnola A, Tomé D, Kaufman DA, Tavella E, Pieretto M, Messina A, De Luca D, Bellaiche M, Mosca A, Piloquet H. Role of lactoferrin in neonates and infants: an update. Am J Perinatol. 2018;35:561–5.CrossRefPubMed
17.
Nordenvall KJ, Brännström M, Malmgren O. Etching of deciduous teeth and young and old permanent teeth: a comparison between 15 and 60 seconds of etching. Am J Orthod. 1980;78:99–108.CrossRefPubMed
18.
Hausdörfer T, Harms L, Kanzow P, Hülsmann M. Three visual–diagnostic methods for the detection of enamel cracks: an in vitro study. J Clin Med. 2023;12:973.CrossRefPubMedPubMedCentral
19.
George L, Baby A, Dhanapal TP, Charlie KM, Joseph A, Varghese AA. Evaluation and comparison of the microhardness of enamel after bleaching with fluoride free and fluoride containing carbamide peroxide bleaching agents and post bleaching anticay application: an in vitro study. Contemp Clin Dent. 2015;6:163–6.CrossRef
20.
Sandhu SV, Tiwari R, Bhullar RK, Bansal H, Bhandari R, Kakkar T, Bhusri R. Sterilization of extracted human teeth: a comparative analysis. J Oral Biol Craniofac Res. 2012;2:170–5.CrossRefPubMedPubMedCentral
21.
Salem-Milani A, Zand V, Asghari-Jafarabadi M, Zakeri-Milani P, Banifatemeh A. The effect of protocol for disinfection of extracted teeth recommended by center for Disease control (CDC) on microhardness of enamel and dentin. J Clin Exp Dent. 2015;7:e552.PubMedPubMedCentral
22.
Secilmis A, Dilber E, Ozturk N, Yilmaz F. G.The effect of storage solutions on mineral content of enamel. Mater Sci Appl. 2013;4:439–45.
23.
Sato Y, Sato T, Niwa M, Aoki H. Precipitation of octacalcium phosphates on artificial enamel in artificial saliva. J Mater Sci Mater Med. 2006;17:1173–7.CrossRefPubMed
24.
Mohamed AM, Wong KH, Lee WJ, Nor MM, Hussaini HM, Rosli TI. In vitro study of white spot lesion: Maxilla and mandibular teeth. Saudi Dent J. 2018;30:142–50.CrossRefPubMedPubMedCentral
25.
26.
Tanthanuch S, Kukiattrakoon B, Thongsroi T, Saesaw P, Pongpaiboon N, Saewong S. In vitro surface and color changes of tooth-colored restorative materials after sport and energy drink cyclic immersions. BMC Oral Health. 2022;22:578.CrossRefPubMedPubMedCentral
27.
Ferreira RI, Haiter-Neto F, Tabchoury CP, Bóscolo FN. In vitro induction of enamel subsurface demineralization for evaluation of diagnostic imaging methods. J Appl Oral Sci. 2007;15:392–8.CrossRefPubMedPubMedCentral
28.
Yılmaz N, Baygin O, Cakıroglu TN, Tüzüner T, Deger O. In vitro evaluation of the effects of frequently prescribed pediatric Drugs on the microhardness of permanent tooth enamel. Dent Med Probl. 2019;56:131–6.CrossRefPubMed
29.
Saeed S, Bshara N, Trak J, Mahmoud G. An in vitro analysis of the cariogenic and erosive potential of pediatric liquid analgesics. J Indian Soc Pedod Prev Dent. 2015;33:143–6.CrossRefPubMed
30.
Pasdar N, Alaghehmand H, Mottaghi F, Tavassoli M. Experimental study of iron and multivitamin drops on enamel microhardness of primary tooth. J Int Soc Prev Community Dent. 2015;5:518.CrossRefPubMedPubMedCentral
31.
Mali GV, Dodamani AS, Karibasappa GN, Kumar PV, Jain VM. Effect of conventional and sugar free pediatric syrup formulations on primary tooth enamel hardness: an: in vitro: study. J Indian Soc Pedod Prev Dent. 2015;33:331–6.CrossRefPubMed
32.
Dhillon SN, Deshpande AN, Macwan C, Patel KS, Shah YS, Jain AA. Comparative evaluation of microhardness and enamel solubility of treated surface enamel with resin infiltrant, fluoride varnish, and casein phosphopeptide-amorphous calcium phosphate: an in vitro study. Int J Clin Pediatr Dent. 2020;13:14–25.CrossRef
33.
Adhani R, Sukmana BI, Suhartono E. Effect pH on demineralization dental erosion. IJCEA. 2015;6:138–41.CrossRef
34.
Mahmoud EF, Omar OM. Erosive and cariogenic potential of various pediatric liquid medicaments on primary tooth enamel: a SEM study. Dent Med Probl. 2018;55:247–54.CrossRefPubMed
35.
Kulkarni P, Anand A, Bansal A, Jain A, Tiwari U, Agrawal S. Erosive effects of pediatric liquid medicinal syrups on primary enamel: an in vitro comparative study. Indian J Dent. 2016;7:131.CrossRefPubMedPubMedCentral
36.
Veeramani R, Shanbhog R, Bhojraj N, Kaul S, Anoop NK. Evaluation of mineral loss in primary and permanent human enamel samples subjected to chemical demineralization by international caries detection and assessment system II and quantitative light-induced fluorescence™: an: in vitro: study. J Indian Soc Pedod Prev Dent. 2020;38:355–60.PubMed
37.
Mahmoud NM, ELmalt MA, Mohamed EA. Evaluation of the erosive effect of pediatric liquid medicinal syrups on primary and permanent enamel. ADJG. 2022;9:531–9.
Metadata
Title
Effect of lactoferrin on enamel characteristics of primary and permanent teeth: an in-vitro study
Authors
Nouran Samir Mohamed Atia
Reham Ahmed El-nemr
Asmaa Ali Emam Abo-Elsoud
Publication date
01-12-2023
Publisher
BioMed Central
Published in
BMC Oral Health / Issue 1/2023
Electronic ISSN: 1472-6831
DOI
https://doi.org/10.1186/s12903-023-03709-1

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