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01-10-2015 | Original Article

Effect of amino acid sequence variations at position 149 on the fusogenic activity of the subtype B avian metapneumovirus fusion protein

Authors: Bingling Yun, Yanni Gao, Yongzhen Liu, Xiaolu Guan, Yongqiang Wang, Xiaole Qi, Honglei Gao, Changjun Liu, Hongyu Cui, Yanping Zhang, Yulong Gao, Xiaomei Wang

Published in: Archives of Virology | Issue 10/2015

Abstract

The entry of enveloped viruses into host cells requires the fusion of viral and cell membranes. These membrane fusion reactions are mediated by virus-encoded glycoproteins. In the case of avian metapneumovirus (aMPV), the fusion (F) protein alone can mediate virus entry and induce syncytium formation in vitro. To investigate the fusogenic activity of the aMPV F protein, we compared the fusogenic activities of three subtypes of aMPV F proteins using a TCSD₅₀ assay developed in this study. Interestingly, we found that the F protein of aMPV subtype B (aMPV/B) strain VCO3/60616 (aMPV/vB) was hyperfusogenic when compared with F proteins of aMPV/B strain aMPV/f (aMPV/fB), aMPV subtype A (aMPV/A), and aMPV subtype C (aMPV/C). We then further demonstrated that the amino acid (aa) residue 149F contributed to the hyperfusogenic activity of the aMPV/vB F protein. Moreover, we revealed that residue 149F had no effect on the fusogenic activities of aMPV/A, aMPV/C, and human metapneumovirus (hMPV) F proteins. Collectively, we provide the first evidence that the amino acid at position 149 affects the fusogenic activity of the aMPV/B F protein, and our findings will provide new insights into the fusogenic mechanism of this protein.

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Title: Effect of amino acid sequence variations at position 149 on the fusogenic activity of the subtype B avian metapneumovirus fusion protein
Authors: Bingling Yun
Yanni Gao
Yongzhen Liu
Xiaolu Guan
Yongqiang Wang
Xiaole Qi
Honglei Gao
Changjun Liu
Hongyu Cui
Yanping Zhang
Yulong Gao
Xiaomei Wang
Publication date: 01-10-2015
Publisher: Springer Vienna
Published in: Archives of Virology / Issue 10/2015
Print ISSN: 0304-8608
Electronic ISSN: 1432-8798
DOI: https://doi.org/10.1007/s00705-015-2524-x

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