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BMC Complementary Medicine and Therapies
Published in: BMC Complementary Medicine and Therapies 1/2016

Open Access 01-12-2016 | Research article

Effect of β-sitosterol against methyl nitrosourea-induced mammary gland carcinoma in albino rats

Authors: Chetan Manral, Subhadeep Roy, Manjari Singh, Swetlana Gautam, Rajnish K. Yadav, Jitendra K Rawat, Uma Devi, Md Nazam Ansari, Abdulaziz S. Saeedan, Gaurav Kaithwas

Published in: BMC Complementary Medicine and Therapies | Issue 1/2016

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Abstract

Background

The present study was in quested to study the effects of β-sitosterol on methyl nitrosourea (MNU) induced mammary gland carcinoma in albino wistar rats.

Methods

Animals were randomized and divided into four groups of eight animals each. Group I (sham control 1 % CMC in normal saline p.o.); Group II (toxic control, MNU 47 mg/kg, i.v); Group III (MNU 47 mg/kg, i.v + β-sitosterol, 10 mg/kg, p.o); Group IV (MNU 47 mg/kg, i.v + β-sitosterol, 20 mg/kg, p.o). Toxicity was induced by single i.v. injection of MNU followed by β-sitosterol supplementation therapy for 115 days at the dose mentioned above.

Results

Treatment with β-sitosterol evidenced decrease in the alveolar bud and lobule score in the whole mount of the mammary gland. β-sitosterol exhibited diminishing effect on oxidative stress through synchronizing lipid and enzymatic antioxidant defense. A significant decrease in the saturated and unsaturated fatty acid was evident with the MNU treatment and β-sitosterol demonstrated a marked effect on it. Pgp 9.5 expression was dose dependently upregulated by β-sitosterol treatment in comparison to MNU treatment. On the contrary, downregulated NF-kB expression was perceived, when β-sitosterol was concomitantly administered with MNU.

Conclusion

β-sitosterol afforded significant protection against the deleterious effects of MNU.
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Metadata
Title
Effect of β-sitosterol against methyl nitrosourea-induced mammary gland carcinoma in albino rats
Authors
Chetan Manral
Subhadeep Roy
Manjari Singh
Swetlana Gautam
Rajnish K. Yadav
Jitendra K Rawat
Uma Devi
Md Nazam Ansari
Abdulaziz S. Saeedan
Gaurav Kaithwas
Publication date
01-12-2016
Publisher
BioMed Central
Published in
BMC Complementary Medicine and Therapies / Issue 1/2016
Electronic ISSN: 2662-7671
DOI
https://doi.org/10.1186/s12906-016-1243-5

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