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Published in: Clinical Orthopaedics and Related Research® 12/2010

01-12-2010 | Editorial

Editorial: The Imperative to Authenticate Cell Lines

Author: John H. Healey, MD, FACS

Published in: Clinical Orthopaedics and Related Research® | Issue 12/2010

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Excerpt

Contamination plagues cell culture research and requires our immediate attention. Cell culture is a powerful tool to investigate the mechanisms of disease, test new drug therapies, and potentially create and engineer tissues. In vitro culture facilitates cancer and stem cell research, in particular. These areas are of great interest to orthopaedic researchers and the subject of many publications in our translational and clinical research journal, Clinical Orthopaedics and Related Research. The integrity of our research and publication effort requires that results are accurate and reproducible. This means that contamination of cell lines must be recognized and eliminated. …
Literature
1.
go back to reference Buehring GC, Eby EA, Eby MJ. Cell line cross-contamination: how aware are Mammalian cell culturists of the problem and how to monitor it? In Vitro Cell Dev Biol Anim. 2004;40:211–215.CrossRefPubMed Buehring GC, Eby EA, Eby MJ. Cell line cross-contamination: how aware are Mammalian cell culturists of the problem and how to monitor it? In Vitro Cell Dev Biol Anim. 2004;40:211–215.CrossRefPubMed
2.
go back to reference Capes-Davis A, Theodosopoulos G, Atkin I, Drexler HG, Kohara A, MacLeod RA, Masters JR, Nakamura Y, Reid YA, Reddel RR, Freshney RI. Check your cultures! A list of cross-contaminated or misidentified cell lines. Int J Cancer. 2010;127:1–8.CrossRefPubMed Capes-Davis A, Theodosopoulos G, Atkin I, Drexler HG, Kohara A, MacLeod RA, Masters JR, Nakamura Y, Reid YA, Reddel RR, Freshney RI. Check your cultures! A list of cross-contaminated or misidentified cell lines. Int J Cancer. 2010;127:1–8.CrossRefPubMed
4.
go back to reference Masters JR, Thomson JA, Daly-Burns B, Reid YA, Dirks WG, Packer P, Toji LH, Ohno T, Tanabe H, Arlett CF, Kelland LR, Harrison M, Virmani A, Ward TH, Ayres KL, Debenham PG. Short tandem repeat profiling provides an international reference standard for human cell lines. Proc Natl Acad Sci USA. 2001;98:8012–8017.CrossRefPubMed Masters JR, Thomson JA, Daly-Burns B, Reid YA, Dirks WG, Packer P, Toji LH, Ohno T, Tanabe H, Arlett CF, Kelland LR, Harrison M, Virmani A, Ward TH, Ayres KL, Debenham PG. Short tandem repeat profiling provides an international reference standard for human cell lines. Proc Natl Acad Sci USA. 2001;98:8012–8017.CrossRefPubMed
5.
go back to reference Røsland GV, Svendsen A, Torsvik A, Sobala E, McCormack E, Immervoll H, Mysliwietz J, Tonn JC, Goldbrunner R, Lønning PE, Bjerkvig R, Schichor C. Long-term cultures of bone marrow-derived human mesenchymal stem cells frequently undergo spontaneous malignant transformation. Cancer Res. 2009;69:5331–5339.CrossRefPubMed Røsland GV, Svendsen A, Torsvik A, Sobala E, McCormack E, Immervoll H, Mysliwietz J, Tonn JC, Goldbrunner R, Lønning PE, Bjerkvig R, Schichor C. Long-term cultures of bone marrow-derived human mesenchymal stem cells frequently undergo spontaneous malignant transformation. Cancer Res. 2009;69:5331–5339.CrossRefPubMed
6.
go back to reference Skloot R. The Immortal Life of Henrietta Lacks. New York, NY: Crown Publishers; 2010:152. Skloot R. The Immortal Life of Henrietta Lacks. New York, NY: Crown Publishers; 2010:152.
7.
go back to reference Torsvik A, Røsland GV, Svendsen A, Molven A, Immervoll H, McCormack E, Lønning PE, Primon M, Sobala E, Tonn JC, Goldbrunner R, Schichor C, Mysliwietz J, Lah TT, Motaln H, Knappskog S, Bjerkvig R. Spontaneous malignant transformation of human mesenchymal stem cells reflects cross-contamination: putting the research field on track—letter. Cancer Res. 2010;70:6393–6396.CrossRefPubMed Torsvik A, Røsland GV, Svendsen A, Molven A, Immervoll H, McCormack E, Lønning PE, Primon M, Sobala E, Tonn JC, Goldbrunner R, Schichor C, Mysliwietz J, Lah TT, Motaln H, Knappskog S, Bjerkvig R. Spontaneous malignant transformation of human mesenchymal stem cells reflects cross-contamination: putting the research field on track—letter. Cancer Res. 2010;70:6393–6396.CrossRefPubMed
Metadata
Title
Editorial: The Imperative to Authenticate Cell Lines
Author
John H. Healey, MD, FACS
Publication date
01-12-2010
Publisher
Springer-Verlag
Published in
Clinical Orthopaedics and Related Research® / Issue 12/2010
Print ISSN: 0009-921X
Electronic ISSN: 1528-1132
DOI
https://doi.org/10.1007/s11999-010-1585-0

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Acknowledgments

Acknowledgment

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