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BMC Complementary Medicine and Therapies

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Open Access 01-12-2017 | Research article

Edible bird’s nest modulate intracellular molecular pathways of influenza A virus infected cells

Authors: Amin Haghani, Parvaneh Mehrbod, Nikoo Safi, Fadzilah A’ini Abd Kadir, Abdul Rahman Omar, Aini Ideris

Published in: BMC Complementary Medicine and Therapies | Issue 1/2017

Abstract

Background

Edible Bird’s Nest (EBN) as a popular traditional Chinese medicine is believed to have health enhancing and antiviral activities against influenza A virus (IAV); however, the molecular mechanism behind therapeutic effects of EBN is not well characterized.

Methods

In this study, EBNs that underwent different enzymatic preparation were tested against IAV infected cells. 50% cytotoxic concentration (CC₅₀) and 50% inhibitory concentration (IC₅₀) of the EBNs against IAV strain A/Puerto Rico/8/1934(H1N1) were determined by HA and MTT assays. Subsequently, the sialic acid content of the used EBNs were analyzed by fluorometric HPLC. Western Blotting and immunofluorescent staining were used to investigate the effects of EBNs on early endosomal trafficking and autophagy process of influenza virus.

Results

This study showed that post inoculations of EBNs after enzymatic preparations have the highest efficacy to inhibit IAV. While CC50 of the tested EBNs ranged from 27.5–32 mg/ml, the IC50 of these compounds ranged between 2.5–4.9 mg/ml. EBNs could inhibit IAV as efficient as commercial antiviral agents, such as amantadine and oseltamivir with different mechanisms of action against IAV. The antiviral activity of these EBNs correlated with the content of N-acetyl neuraminic acid. EBNs could affect early endosomal trafficking of the virus by reducing Rab5 and RhoA GTPase proteins and also reoriented actin cytoskeleton of IAV infected cells. In addition, for the first time this study showed that EBNs can inhibit intracellular autophagy process of IAV life cycle as evidenced by reduction of LC3-II and increasing of lysosomal degradation.

Conclusions

The results procured in this study support the potential of EBNs as supplementary medication or alternative to antiviral agents to inhibit influenza infections. Evidently, EBNs can be a promising antiviral agent; however, these natural compounds should be screened for their metabolites prior to usage as therapeutic approach.

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Title: Edible bird’s nest modulate intracellular molecular pathways of influenza A virus infected cells
Authors: Amin Haghani
Parvaneh Mehrbod
Nikoo Safi
Fadzilah A’ini Abd Kadir
Abdul Rahman Omar
Aini Ideris
Publication date: 01-12-2017
Publisher: BioMed Central
Published in: BMC Complementary Medicine and Therapies / Issue 1/2017
Electronic ISSN: 2662-7671
DOI: https://doi.org/10.1186/s12906-016-1498-x

Keynote webinar | Spotlight on medication adherence

Springer Medicine

Edible bird’s nest modulate intracellular molecular pathways of influenza A virus infected cells

Abstract

Background

Methods

Results

Conclusions

Keynote webinar | Spotlight on medication adherence

Springer Medicine

Abstract

Background

Methods

Results

Conclusions

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