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European Journal of Nuclear Medicine and Molecular Imaging

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01-08-2007 | Original Article

Early detection and monitoring of cartilage alteration in the experimental meniscectomised guinea pig model of osteoarthritis by ^99mTc-NTP 15-5 scintigraphy

Authors: Elisabeth Miot-Noirault, Aurélien Vidal, Philippe Pastoureau, Jacques Bonafous, Agnès Chomel, Laurent Sarry, Laurent Audin, Jean-Claude Madelmont, Nicole Moins

Published in: European Journal of Nuclear Medicine and Molecular Imaging | Issue 8/2007

Abstract

Purpose

This study in the meniscectomised guinea pig aimed to demonstrate that the radiotracer ^99mTc-NTP 15-5 would have pathophysiological validity for in vivo osteoarthritis imaging.

Methods

The specificity of ^99mTc-NTP 15-5 for cartilage was determined in healthy animals (n = 13), by tissue radioactivity counting, joint autoradiography and scintigraphy. ^99mTc-NTP 15-5 scintigraphy was performed at 20, 50, 80, 115, 130, 150 and 180 days after medial meniscectomy (n = 10 MNX) or sham operation (n = 5), and scintigraphic ratios (operated/contralateral) were calculated for femoral (F) and tibial (T) areas. F and T ratios were compared with those of ^99mTc-MDP bone scintigraphy. At the study end-point, autoradiographic analysis of joint ^99mTc-NTP 15-5 distribution and macroscopic scoring of cartilage integrity were performed.

Results

The high and specific accumulation of ^99mTc-NTP 15-5 in normal cartilage (about 5.5 ± 1.7 % of injected dose/g of tissue), which permitted joint imaging with high contrast, was affected by osteoarthritis. In the MNX group, ^99mTc-NTP 15-5 accumulation in cartilage within the operated joint, relative to the contralateral joint, was observed to change in the same animals as pathology progressed. Although F and T ratios were significantly higher in MNX (F = 1.7 ± 0.2; T = 1.6 ± 0.1) than in shams (F = 1.0 ± 0.1; T = 1.0 ± 0.1) at day 50, they were significantly lower in MNX (F = 0.6 ± 0.1; T = 0.7 ± 0.1) than in shams (F = 1.0 ± 0.1; T = 0.9 ± 0.1) at day 180. No change in ^99mTc-MDP uptake was observed over 6 months. Macroscopic analysis confirmed features of osteoarthritis only in MNX knees.

Conclusion

These results in MNX guinea pigs provide additional support for the use of ^99mTc-NTP 15-5 for in vivo imaging of osteoarthritis.

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Title: Early detection and monitoring of cartilage alteration in the experimental meniscectomised guinea pig model of osteoarthritis by 99mTc-NTP 15-5 scintigraphy
Authors: Elisabeth Miot-Noirault
Aurélien Vidal
Philippe Pastoureau
Jacques Bonafous
Agnès Chomel
Laurent Sarry
Laurent Audin
Jean-Claude Madelmont
Nicole Moins
Publication date: 01-08-2007
Publisher: Springer-Verlag
Published in: European Journal of Nuclear Medicine and Molecular Imaging / Issue 8/2007
Print ISSN: 1619-7070
Electronic ISSN: 1619-7089
DOI: https://doi.org/10.1007/s00259-006-0320-2

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Early detection and monitoring of cartilage alteration in the experimental meniscectomised guinea pig model of osteoarthritis by ^99mTc-NTP 15-5 scintigraphy

Abstract

Purpose

Methods

Results

Conclusion

Keynote webinar | Spotlight on medication adherence

Springer Medicine

Abstract

Purpose

Methods

Results

Conclusion

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