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01-03-2006 | Original Article

E. coli virulence factors in children with neurogenic bladder associated with bacteriuria

Authors: Eliana B. M. Guidoni, Vanessa A. Dalpra, Patrícia Maria Figueiredo, Domingos da Silva Leite, Lycia Mara J. Mímica, Tomomasa Yano, Jesus E. Blanco, Julio Toporovski

Published in: Pediatric Nephrology | Issue 3/2006

Abstract

The value of E. coli virulence factors in patients with neurogenic bladder has not been established. The aim of this study is to correlate E. coli virulence factors with asymptomatic and symptomatic UTI in children with neurogenic bladder. Fifty E. coli strains, which were collected in sequence, underwent analysis in relation to: the association to pyuria, serotype (O:H), the presence of genes and expression of fimbriae P, type 1, S and hemagglutinin Dr, the presence of the gene and production of hemolysins and cytotoxins (CNF1). We also analyzed the cell adherence capability and pattern and presence of usp (uropathogenic-specific protein). Pyuria was present in most of the positive urine cultures, with 86% AB and 97% UTI. Low rates of uropathogenic strains were observed in the two groups, with 18% AB and 21% UTI. Type 1 fimbria predominated in 44% of the E. coli strains. Of the bacteria studied, 30% (15 strains) exhibited papG genotypes (11 class II and 4 class III). Of these, 12/15 patients presented AB. Production of hemolysins was detected in 38% of the strains (16 AB and 3 UTI) and usp in only 18% of the strains, with 8 AB and 1 UTI. Adherence tests demonstrated the adhesive capacity in all samples analyzed. Neither group (AB or symptomatic UTI) presented a statistically significant difference in relation to the virulence factors studied. E. coli clones that caused symptomatic UTI in children with neurogenic bladder expressed few virulence factors, with no statistically significant difference in comparison to the AB group.

Lapides J, Diokno AC, Silber SJ, Lowe BS (1972) Clean, intermittent self-catheterization in the treatment of urinary tract disease. J Urol 107:458–461PubMed

Joseph DB, Bauer SB, Colodny AH, Mandell J, Retik AB (1989) Clean, intermittent catheterization of infants with neurogenic bladder. Pediatrics 84:78–82PubMed

Kass EJ, Koff SA, Diokno AC, Lapides J (1981) The significance of bacilluria in children on long-term intermittent catheterization. J Urol 126:223–225PubMed

Schlager TA, Dilks S, Trudell J, Whittam TS, Hendley JO (1995) Bacteriuria in children with neurogenic bladder treated with intermittent catheterization: natural history. J Pediatr 126:490–496CrossRefPubMed

Schlager TA, Whittam TS, Hendley JO, Wilson RA, Bhang J, Grady R, Stapleton A (2000) Expression of virulence factors among Escherichia coli isolated from the periurethra and urine of children with neurogenic bladder on intermittent catheterization. Pediatr Infect Dis J 19:37–41CrossRefPubMed

Brem AS, Martin D, Callaghan J, Maynard J (1987) Long-term renal risk factors in children with meningomyelocele. J Pediatr 110:51–55PubMed

Schlager TA, Dilks SA, Lohr JA, Hayden GF, Kopco JA, Hendley JW (1992) Periurethral colonization and urinary leukocytes as markers for bacteriuria in children with neurogenic bladder. Urol Res 20:361–363CrossRefPubMed

Stöher M, Castro- Diaz D, Chartier-Kartler E (2003) Guidelines on neurogenic lower urinary tract dysfunction. European Association of Urology (cited 2003 Feb 2). Available from: http//www.uroweb.org/files/uploaded_files/guidelines/neurogenic.pdf

Hellerstein S (1982) Recurrent urinary tract infections in children. Pediatr Infect Dis 1:271–281PubMed

10.

Kass EH (1956) Asymptomatic infections of the urinary tract. Trans Assoc Am Physicians 69:56–64PubMed

11.

Blanco J, Blanco M, Alonso MP (1992) A Factores de virulência de los Escherichia coli causantes de infecciones extraintestinales. Monografias da Universidade de Compostela no 170:87–90

12.

Evans, DJ Jr, Evans DG, Young LS, Pitt J (1981) Hemagglutination typing of Escherichia coli: definition of seven hemagglutination types. J Clin Microbiol 12:235–242

13.

Gander RM, Thomas VL, Forland M (1985) Mannose-resistant hemagglutination and P receptor recognition of uropathogenic Escherichia coli isolated from adult patients. J Infect Dis 151:508–513PubMed

14.

Blanco J, Blanco M, Gonzalez EA, Alonso MP, Garabal JI (1990) Comparative evaluation of three tests for the detection of Escherichia coli cytotoxic necrotizing factors (CNF1 and CNF2) using filtrates of cultures treated with mitomycin C. FEMS Microbiol Lett 57:311–316CrossRefPubMed

15.

Scaletsky IC, Silva, ML, Trabulsi LR (1984) Distinctive patterns of adherence of enteropathogenic Escherichia coli to HeLa cells. Infect Immun 45:534–536PubMed

16.

Blanco M, Blanco JE, Rodrigues E (1997) Detection of virulence genes in uropathogenic Escherichia coli by polymerase chain reaction (PCR): comparison with results obtained using phenotypic methods. J Microbiol Methods 31:37–43CrossRef

17.

Yamamoto S, Terai A, Yuri K, Kurazono H, Takeda Y, Yoshida O (1995) Detection of urovirulence factors in Escherichia coli by multiplex polymerase chain reaction. FEMS Immunol Med Microbiol 12:85–90CrossRefPubMed

18.

Daigle F, Harel J, Fairbrother JM, Lebel P (1994) Expression and detection of pap-, sfa-, and afa-encoded fimbrial adhesin systems among uropathogenic Escherichia coli. Can J Microbiol 40:286–291PubMed

19.

Pickett CL, Twiddy EM, Coker C, Holmes RK (1989) Cloning, nucleotide sequence, and hybridization studies of the type IIb heat-labile enterotoxin gene of Escherichia coli. J Bacteriol 171:4945–4952PubMed

20.

Karkkianen UM, Kauppinen J, Ikaheimo R (1998) A Rapid and specific detection of three different G adhesin classes of P-fimbriae in uropathogenic Escherichia coli by polimerase chain reaction. J Microl Method 34:23–29CrossRef

21.

Kurazono H, Yamamoto S, Nakano M, Nair GB, Terai A, Chaicumpa W, Hayashi H (2000) Characterization of a putative virulence island in the chromosome of uropathogenic Escherichia coli possessing a gene encoding a uropathogenic-specific protein. Microb Pathog 28:183–189CrossRefPubMed

22.

Rosner B (1986) Fundamentals of biostatistics, 2nd edn. PWS Publishers, Boston

23.

Sotolongo JR Jr, Koleilat N (1990) Significance of asymptomatic bacteriuria in spinal cord injury patients on condom catheter. J Urol 143:979–980PubMed

24.

Andersson P, Engberg I, Lidin-Janson G, Lincoln K, Hull R, Hull S, Svanborg C (1991) Persistence of Escherichia coli bacteriuria is not determined by bacterial adherence. Infect Immun 59:2915–2921PubMed

25.

Gribble MJ, McCallum NM, Schechter MT (1988) Evaluation of diagnostic criteria for bacteriuria in acutely spinal cord injured patients undergoing intermittent catheterization. Diagn Microbiol Infect Dis 9:197–206CrossRefPubMed

26.

Schlager TA, Grady R, Mills SE, Hendley JO (2004) Bladder epithelium is abnormal in patients with neurogenic bladder due to myelomeningocele. Spinal Cord 42:163–168CrossRefPubMed

27.

Orskov F, Sharma V, Orskov I (1984) Influence of growth temperature on the development of Escherichia coli polysaccharide K antigens. J Gen Microbiol 130:2681–2684PubMed

28.

Hansson S, Jodal U, Lincoln K, Svanborg-Eden C (1989) Untreated asymptomatic bacteriuria in girls: II--Effect of phenoxymethylpenicillin and erythromycin given for intercurrent infections. BMJ 298:856–859PubMed

29.

Eisenstein BI (1981) Phase variation of Type 1 fimbriae in Escherichia coli is under transcriptional control. Science 214:337–339PubMed

30.

Miyazaki J, Ba-Thein W, Kumao T, Obata Yasuoka M, Akaza H, Hayshi H (2002) Type 1, P and S fimbriae, and afimbrial adhesin I are not essential for uropathogenic Escherichia coli to adhere to and invade bladder epithelial cells. FEMS Immunol Med Microbiol 33:23–26CrossRefPubMed

31.

Mobley HL, Chippendale GR, Tenney JH, Hull RA, Warren JW (1987) Expression of type 1 fimbriae may be required for persistence of Escherichia coli in the catheterized urinary tract. J Clin Microbiol 25:2253–2257PubMed

32.

Plos K, Connell H, Jodal U, Marklund BI, Marild S, Wettergren B, Svanborg C (1995) Intestinal carriage of P fimbriated Escherichia coli and the susceptibility to urinary tract infection in young children. J Infect Dis 171:625–631PubMed

33.

Puente JL, Finlay BB (2001) Pathogenic Escherichia coli. In: Groisman EA (ed) Principles of bacterial pathogenesis. Academic Press, San Diego, pp 387–457

34.

Roberts JA, Marklund BI, Ilver D, Haslam D, Kaack MB, Baskin G, Louis M, Mollby R, Winberg J, Normark S (1994) The Gal(alpha 1–4)Gal-specific tip adhesin of Escherichia coli P-fimbriae is needed for pyelonephritis to occur in the normal urinary tract. Proc Natl Acad Sci USA 91:11889–11893PubMed

35.

Tseng CC, Huang JJ, Ko WC, Yan JJ, Wu JJ (2001) Decreased predominance of papG class II allele in Escherichia coli strains isolated from adults with acute pyelonephritis and urinary tract abnormalities. J Urol 166:1643–1646CrossRefPubMed

36.

Andreu A, Stapleton AE, Fennell C, Lockman HA, Xercavins M, Fernandez F, Stamm WE (1997) Urovirulence determinants in Escherichia coli strains causing prostatitis. J Infect. Dis 176:464–469

37.

Foxman B, Zhang L, Palin K, Tallman P, Marrs CF (1995) Bacterial virulence characteristics of Escherichia coli isolates from first-time urinary tract infection. J Infect Dis 171:1514–1521PubMed

38.

Nakano M, Yamamoto S, Terai A, Ogawa O, Makino SI, Hayashi H, Nair GB, Kurazono H (2001) Structural and sequence diversity of the pathogenicity island of uropathogenic Escherichia coli which encodes the USP protein. FEMS Microbiol Lett 205:71–76CrossRefPubMed

Title: E. coli virulence factors in children with neurogenic bladder associated with bacteriuria
Authors: Eliana B. M. Guidoni
Vanessa A. Dalpra
Patrícia Maria Figueiredo
Domingos da Silva Leite
Lycia Mara J. Mímica
Tomomasa Yano
Jesus E. Blanco
Julio Toporovski
Publication date: 01-03-2006
Publisher: Springer-Verlag
Published in: Pediatric Nephrology / Issue 3/2006
Print ISSN: 0931-041X
Electronic ISSN: 1432-198X
DOI: https://doi.org/10.1007/s00467-005-2133-0

Keynote webinar | Spotlight on medication adherence

Springer Medicine

E. coli virulence factors in children with neurogenic bladder associated with bacteriuria

Abstract

Keynote webinar | Spotlight on medication adherence

Springer Medicine

Abstract

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