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Journal of Assisted Reproduction and Genetics

Published in:

01-07-2016 | Gamete Biology

Dynamic changes in mitochondrial distribution in human oocytes during meiotic maturation

Authors: Yuki Takahashi, Shu Hashimoto, Takayuki Yamochi, Hiroya Goto, Masaya Yamanaka, Ami Amo, Hiroshi Matsumoto, Masayasu Inoue, Keijiro Ito, Yoshiharu Nakaoka, Nao Suzuki, Yoshiharu Morimoto

Published in: Journal of Assisted Reproduction and Genetics | Issue 7/2016

Abstract

Purpose

The change of mitochondrial distribution in human oocytes during meiotic maturation was assessed using 223 human oocytes donated from patients undergoing fertility treatment between June 2013 and February 2016.

Methods

Live cell images of fluorescence-labelled mitochondria in human oocytes were analysed to investigate dynamic changes in mitochondrial distribution during meiotic maturation using a confocal microscope combined with an incubator in the presence or absence of colchicine and cytochalasin B, inhibitors for tubulin and actin filament, respectively. Subcellular distribution of mitochondria in human oocytes was also assessed at various stages using a transmission electron microscope (TEM).

Results

Live cell imaging analysis revealed that the mitochondria-occupied cytoplasmic area decreased from 83 to 77 % of the total cytoplasmic area around 6 h before germinal vesicle breakdown (GVBD) and that mitochondria accumulated preferentially close to the perinuclear region. Then, the mitochondria-distributed area rapidly increased to 85 % of total cytoplasm at the time of GVBD. On the other hand, there was no significant change in mitochondrial distribution before and after polar body extrusion. Such changes in mitochondrial localization were affected differently by colchicine and cytochalasin B. Most of mitochondria in the cytoplasm formed cluster-like aggregates before GVBD while they distributed homogeneously after GVBD.

Conclusions

Most mitochondria localized predominantly in the non-cortical region of the cytoplasm of GV stage-oocytes, while the mitochondria-occupied area decreased transiently before GVBD and increased rapidly to occupy the entire area of the cytoplasm at GVBD by some cytoskeleton-dependent mechanism.

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Title: Dynamic changes in mitochondrial distribution in human oocytes during meiotic maturation
Authors: Yuki Takahashi
Shu Hashimoto
Takayuki Yamochi
Hiroya Goto
Masaya Yamanaka
Ami Amo
Hiroshi Matsumoto
Masayasu Inoue
Keijiro Ito
Yoshiharu Nakaoka
Nao Suzuki
Yoshiharu Morimoto
Publication date: 01-07-2016
Publisher: Springer US
Published in: Journal of Assisted Reproduction and Genetics / Issue 7/2016
Print ISSN: 1058-0468
Electronic ISSN: 1573-7330
DOI: https://doi.org/10.1007/s10815-016-0716-2

Keynote webinar | Spotlight on medication adherence

Springer Medicine

Dynamic changes in mitochondrial distribution in human oocytes during meiotic maturation

Abstract

Purpose

Methods

Results

Conclusions

Keynote webinar | Spotlight on medication adherence

Springer Medicine

Abstract

Purpose

Methods

Results

Conclusions

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