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01-06-2004 | Technical Note

Duplication of DYS19 flanking regions in other parts of the Y chromosome

Authors: John M. Butler, Richard Schoske

Published in: International Journal of Legal Medicine | Issue 3/2004

Abstract

During the testing of alternative primers for the Y chromosome short tandem repeat marker DYS19, a duplicated region of the Y chromosome was discovered. The duplicated sequence is contained within GenBank accession AC006335 and has a high degree of homology with the DYS19 flanking region (GenBank accession AC017019) but without the polymorphic TAGA repeat. Bioinformatic approaches have been taken to try and understand the implications of this homolog to enable improved primer design for DYS19. Sequence alignments and careful placement of primers in order to obtain specific amplification of the DYS19 locus are discussed in the context of all previously published primer sets. Since the DYS19 locus is part of the widely used minimal haplotype, its robust amplification is highly desirable particularly in multiplex reactions. The discovery of this duplicated region of the Y chromosome shows the value of newly available human genome sequence information for assay design and the importance of using sequence queries and alignments in the primer design process.

The accepted SI unit of concentration, mol/l has been represented by the symbol M in order to conform to the conventions of this journal.

Certain commercial equipment, instruments and materials are identified in order to specify experimental procedures as completely as possible. In no case does such identification imply a recommendation or endorsement by the National Institute of Standards and Technology or the US Department of Defense, nor does it imply that any of the materials, instruments or equipment identified are necessarily the best available for the purpose.

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Title: Duplication of DYS19 flanking regions in other parts of the Y chromosome
Authors: John M. Butler
Richard Schoske
Publication date: 01-06-2004
Publisher: Springer-Verlag
Published in: International Journal of Legal Medicine / Issue 3/2004
Print ISSN: 0937-9827
Electronic ISSN: 1437-1596
DOI: https://doi.org/10.1007/s00414-004-0436-5

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Duplication of DYS19 flanking regions in other parts of the Y chromosome

Abstract

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