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Lasers in Medical Science
Published in: Lasers in Medical Science 6/2020

01-08-2020 | Original Article

Dual effect of blue light on Fusariumsolani clinical corneal isolates in vitro

Authors: Yingyu Li, Pei Zhang, Chen Huang, Wei Wang

Published in: Lasers in Medical Science | Issue 6/2020

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Abstract

The purpose was to investigate the effect of daylight-intensity blue light on F. solani isolated from the cornea of patients with fungal keratitis. Spore suspensions of 5 F. solani strains (one standard strain and 4 clinical corneal isolates) were prepared in 6-well plates. Blue light groups were irradiated by a light-emitting diode (LED) device with a peak wavelength of 454 nm at 0.5 mW/cm2 for 0 to 48 h, while the controls were maintained in darkness. Hyphal morphology in the 6-well plates was recorded at 0, 12, 24, 36, 48 h. One hundred microliters of spore suspensions of each strain at these five time points was transferred to SGA plates and cultured for 36 h at 29 °C; the number of colonies formed was counted as a measure of conidia quality and viability. Blue light has dual effects on F. solani. The hyphal length of F. solani exposed to blue light was significantly shorter than that of the control (P < 0.01), indicating that fungal growth was inhibited. Meanwhile, instead of reducing the viability of spores, blue light significantly enhanced the conidia quality and viability after at least 24 h irradiation. Daylight-intensity blue light exposure will inhibit the hyphal growth of F. solani but promote conidiation, which would be more harmful to fungal keratitis. Eliminating the influence of blue light for these patients should be taken into account.
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Metadata
Title
Dual effect of blue light on Fusariumsolani clinical corneal isolates in vitro
Authors
Yingyu Li
Pei Zhang
Chen Huang
Wei Wang
Publication date
01-08-2020
Publisher
Springer London
Published in
Lasers in Medical Science / Issue 6/2020
Print ISSN: 0268-8921
Electronic ISSN: 1435-604X
DOI
https://doi.org/10.1007/s10103-019-02911-4

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