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Open Access 01-12-2011 | Research

Downregulation of connective tissue growth factor inhibits the growth and invasion of gastric cancer cells and attenuates peritoneal dissemination

Authors: Cheng-Gang Jiang, Ling Lv, Fu-Rong Liu, Zhen-Ning Wang, Fu-Nan Liu, Yan-Shu Li, Chun-Yu Wang, Hong-Yan Zhang, Zhe Sun, Hui-Mian Xu

Published in: Molecular Cancer | Issue 1/2011

Abstract

Background

Connective tissue growth factor (CTGF) has been shown to be implicated in tumor development and progression. However, the role of CTGF in gastric cancer remains largely unknown.

Results

In this study, we showed that CTGF was highly expressed in gastric cancer tissues compared with matched normal gastric tissues. The CTGF expression in tumor tissue was associated with histologic grade, lymph node metastasis and peritoneal dissemination (P < 0.05). Patients with positive CTGF expression had significantly lower cumulative postoperative 5 year survival rate than those with negative CTGF expression (22.9% versus 48.1%, P < 0.001). We demonstrated that knockdown of CTGF expression significantly inhibited cell growth of gastric cancer cells and decreased cyclin D₁ expression. Moreover, knockdown of CTGF expression also markedly reduced the migration and invasion of gastric cancer cells and decreased the expression of matrix metalloproteinase (MMP)-2 and MMP-9. Animal studies revealed that nude mice injected with the CTGF knockdown stable cell lines featured a smaller number of peritoneal seeding nodules than the control cell lines.

Conclusions

These data suggest that CTGF plays an important role in cell growth and invasion in human gastric cancer and it appears to be a potential prognostic marker for patients with gastric cancer.

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Title: Downregulation of connective tissue growth factor inhibits the growth and invasion of gastric cancer cells and attenuates peritoneal dissemination
Authors: Cheng-Gang Jiang
Ling Lv
Fu-Rong Liu
Zhen-Ning Wang
Fu-Nan Liu
Yan-Shu Li
Chun-Yu Wang
Hong-Yan Zhang
Zhe Sun
Hui-Mian Xu
Publication date: 01-12-2011
Publisher: BioMed Central
Published in: Molecular Cancer / Issue 1/2011
Electronic ISSN: 1476-4598
DOI: https://doi.org/10.1186/1476-4598-10-122

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Abstract

Background

Results

Conclusions

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