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Translational Neurodegeneration
Published in: Translational Neurodegeneration 1/2018

Open Access 01-12-2018 | Research

Dopaminergic neurons show increased low-molecular-mass protein 7 activity induced by 6-hydroxydopamine in vitro and in vivo

Authors: Ming-Shu Mo, Gui-Hua Li, Cong-Cong Sun, Shu-Xuan Huang, Lei Wei, Li-Min Zhang, Miao-Miao Zhou, Zhuo-Hua Wu, Wen-Yuan Guo, Xin-Ling Yang, Chao-Jun Chen, Shao-Gang Qu, Jian-Xing He, Ping-Yi Xu

Published in: Translational Neurodegeneration | Issue 1/2018

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Abstract

Background

Abnormal expression of major histocompatibility complex class I (MHC-I) is increased in dopaminergic (DA) neurons in the substantia nigra (SN) in Parkinson’s disease (PD). Low-molecular-mass protein 7 (β5i) is a proteolytic subunit of the immunoproteasome that regulates protein degradation and the MHC pathway in immune cells.

Methods

In this study, we investigated the role of β5i in DA neurons using a 6-hydroxydopamine (6-OHDA) model in vitro and vivo.

Results

We showed that 6-OHDA upregulated β5i expression in DA neurons in a concentration- and time-dependent manner. Inhibition and downregulation of β5i induced the expression of glucose-regulated protein (Bip) and exacerbated 6-OHDA neurotoxicity in DA neurons. The inhibition of β5i further promoted the activation of Caspase 3-related pathways induced by 6-OHDA. β5i also activated transporter associated with antigen processing 1 (TAP1) and promoted MHC-I expression on DA neurons.

Conclusion

Taken together, our data suggest that β5i is activated in DA neurons under 6-OHDA treatment and may play a neuroprotective role in PD.
Appendix
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Metadata
Title
Dopaminergic neurons show increased low-molecular-mass protein 7 activity induced by 6-hydroxydopamine in vitro and in vivo
Authors
Ming-Shu Mo
Gui-Hua Li
Cong-Cong Sun
Shu-Xuan Huang
Lei Wei
Li-Min Zhang
Miao-Miao Zhou
Zhuo-Hua Wu
Wen-Yuan Guo
Xin-Ling Yang
Chao-Jun Chen
Shao-Gang Qu
Jian-Xing He
Ping-Yi Xu
Publication date
01-12-2018
Publisher
BioMed Central
Published in
Translational Neurodegeneration / Issue 1/2018
Electronic ISSN: 2047-9158
DOI
https://doi.org/10.1186/s40035-018-0125-9

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