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Journal of Occupational Medicine and Toxicology

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Open Access 01-12-2016 | Research

Does the Low-level occupational exposure to volatile organic compounds alter the seasonal variation of selected markers of oxidative stress? A case–control study in nail technicians

Authors: Peter Grešner, Radosław Świercz, Magdalena Beata Król, Ewa Twardowska, Jolanta Gromadzińska, Wojciech Wąsowicz

Published in: Journal of Occupational Medicine and Toxicology | Issue 1/2016

Abstract

Background

In this study we tested whether the seasonal variations in levels of selected biomarkers of oxidative stress in female nail technicians occupationally exposed to low levels of volatile organic compounds (VOCs) differ significantly from those observed among healthy unexposed controls. Airborne levels of selected VOCs in nail salons were also analyzed and tested for associations with seasonal variations of the levels of biomarkers among nail technicians.

Methods

The study enrolled 145 female nail technicians and 145 healthy unexposed female controls. The airborne VOCs and levels of biomarkers were assessed by GC-MS chromatography and absorption/fluorescence spectrophotometry, respectively.

Results

Plasma levels of thiobarbituric acid reactive species, ceruloplasmin, the activity of glutathione peroxidase (GPx1) and the SOD1/GPx1 activity ratio presented significant differences between the so-called “hot” and “cold” seasons in the case of nail technicians as well as in unexposed controls (p < <0.0001 for all four biomarkers). The pattern of these variations among nail technicians was found to be significantly different compared to that of the control subjects (p < <0.0001). Although such differences might intuitively be attributed to occupational exposure of nail technicians to VOCs, which was found to be higher during the “cold” season compared to the “hot” one, our study provided only limited evidence in favor of the hypothesis, that the different pattern of seasonal variations of biomarkers among nail technicians might have resulted from seasonal fluctuations in their occupational exposure to VOCs.

Conclusion

Further investigation is thus needed in order to elucidate the effect of low-level occupational exposure to VOCs on seasonal variations of biomarkers of oxidative stress.

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Manikantan P, Balachanadar V, Sasikala K, Mohanadevi S, Arun M, Kumar AK, Khan Mustaq Ahamed SA, Krishnan BB, Kumar SS. Cytogenetic methods for assessing human exposure to toluene in Coimbatore, South India. Asian Pac J Cancer Prev. 2010;11:1687–93.PubMed

Tunca BT, Egeli U. Cytogenetic findings on shoe workers exposed long-term to benzene. Environ Health Perspect. 1996;104(Suppl):1313–7.CrossRefPubMedPubMedCentral

Bogadi-Sare A, Brumen V, Turk R, Karacic V, Zavalic M. Genotoxic effects in workers exposed to benzene: with special reference to exposure biomarkers and confounding factors. Ind Health. 1997;35:367–73.CrossRefPubMed

Gerin M, Siemiatycki J, Desy M, Krewski D. Associations between several sites of cancer and occupational exposure to benzene, toluene, xylene, and styrene: results of a case–control study in Montreal. Am J Ind Med. 1998;34:144–56.CrossRefPubMed

Lundberg I, Milatou-Smith R. Mortality and cancer incidence among Swedish paint industry workers with long-term exposure to organic solvents. Scand J Work Environ Health. 1998;24:270–5.CrossRefPubMed

Liu Y, Zhou Q, Xie X, Lin D, Dong L. Oxidative stress and DNA damage in the earthworm Eisenia fetida induced by toluene, ethylbenzene and xylene. Ecotoxicology. 2010;19(8):1551–9.CrossRefPubMed

Singh MP, Mishra M, Sharma A, Shukla AK, Mudiam MKR, Patel DK, Ram KR, Chowdhuri DK. Genotoxicity and apoptosis in Drosophila melanogaster exposed to benzene, toluene and xylene: attenuation by quercetin and curcumin. Toxicol Appl Pharmacol. 2011;253:14–30.CrossRefPubMed

Coskun O, Oter S, Korkmaz A, Armutcu F, Kanter M. The oxidative and morphological effects of high concentration chronic toluene exposure on rat sciatic nerves. Neurochem Res. 2005;30:33–8.CrossRefPubMed

Moro AM, Charão M, Brucker N, Bulcão R, Freitas F, Guerreiro G, Baierle M, Nascimento S, Waechter F, Hirakata V, Linden R, Thiesen FV, Garcia SC. Effects of low-level exposure to xenobiotics present in paints on oxidative stress in workers. Sci Total Environ. 2010;408:4461–7.CrossRefPubMed

10.

Moro AM, Brucker N, Charão M, Bulcão R, Freitas F, Baierle M, Nascimento S, Valentini J, Cassini C, Salvador M, Linden R, Thiesen F, Buffon A, Moresco R, Garcia SC. Evaluation of genotoxicity and oxidative damage in painters exposed to low levels of toluene. Mutat Res. 2012;746:42–8.CrossRefPubMed

11.

Roelofs C, Azaroff LS, Holcroft C, Nguyen H, Doan T. Results from a community-based occupational health survey of Vietnamese-American nail salon workers. J Immigr Minor Health. 2008;10:353–61.CrossRefPubMed

12.

Yu HS, Oyama T, Isse T, Kitagawa K, Pham TT, Tanaka M, Kawamoto T. Formation of acetaldehyde-derived DNA adducts due to alcohol exposure. Chem Biol Interact. 2010;188:367–75.CrossRefPubMed

13.

Grešner P, Stepnik M, Król MB, Swiercz R, Smok-Pieniazek A, Twardowska E, et al. Dysregulation of markers of oxidative stress and DNA damage among nail technicians despite low exposure to volatile organic compounds. Scand J Work Environ Health. 2015;41:579–93.CrossRefPubMed

14.

Lazarov A. Sensitization to acrylates is a common adverse reaction to artificial fingernails. J Eur Acad Dermatol Venereol. 2007;21:169–74.CrossRefPubMed

15.

Quach T, Nguyen K-D, Doan-Billings P-A, Okahara L, Fan C, Reynolds P. A preliminary survey of Vietnamese nail salon workers in Alameda County, California. J Community Health. 2008;33:336–43.CrossRefPubMed

16.

Park S-A, Gwak S, Choi S. Assessment of occupational symptoms and chemical exposures for nail salon technicians in daegu city, Korea. J Prev Med Public Health. 2014;47:169–76.CrossRefPubMedPubMedCentral

17.

Quach T, Gunier R, Tran A, Von Behren J, Doan-Billings P-A, Nguyen K-D, Okahara L, Lui BY-B, Nguyen M, Huynh J, Reynolds P. Characterizing workplace exposures in Vietnamese women working in California nail salons. Am J Public Health. 2011;101(Suppl):S271–6.CrossRefPubMedPubMedCentral

18.

Reutman SR, Rohs AM, Clark JC, Johnson BC, Sammons DL, Toennis CA, Robertson SA, Mackenzie BA, Lockey JE. A pilot respiratory health assessment of nail technicians: symptoms, lung function, and airway inflammation. Am J Ind Med. 2009;875:868–75.CrossRef

19.

Harris-Roberts J, Bowen J, Sumner J, Stocks-Greaves M, Bradshaw L, Fishwick D, Barber CM. Work-related symptoms in nail salon technicians. Occup Med (Lond). 2011;61:335–40.CrossRef

20.

Roelofs C, Do T. Exposure assessment in nail salons: an indoor air approach. ISRN Public Health. 2012;2012:1–7.CrossRef

21.

Central Institute for Labor Protection - National Research Institute. www.ciop.pl.

22.

Konior A, Klemenska E, Brudek M, Podolecka E, Czarnowska E, Beręsewicz A. Seasonal superoxide overproduction and endothelial activation in guinea-pig heart; seasonal oxidative stress in rats and humans. J Mol Cell Cardiol. 2011;50:686–69.CrossRefPubMed

23.

Moller P, Wallin H, Holst E, Knudsen LE. Sunlight-induced DNA damage in human mononuclear cells. FASEB J. 2002;16:45–53.CrossRefPubMed

24.

Møller P, Folkmann JK, Forchhammer L, Bräuner EV, Danielsen PH, Risom L, Loft S. Air pollution, oxidative damage to DNA, and carcinogenesis. Cancer Lett. 2008;266:84–97.CrossRefPubMed

25.

Morera AL, Intxausti A, Abreu-Gonzalez P. Winter/summer seasonal changes in malondialdehyde formation as a source of variance in oxidative stress schizophrenia research. World J Biol Psychiatry. 2009;10:576–80.CrossRefPubMed

26.

Belló-Klein A, Morgan-Martins MI, Barp J, Llesuy S, Belló AA, Singal PK. Circaannual changes in antioxidants and oxidative stress in the heart and liver in rats. Comp Biochem Physiol C Toxicol Pharmacol. 2000;126:203–8.PubMed

27.

Bernabucci U, Ronchi B, Lacetera N, Nardone A. Markers of oxidative status in plasma and erythrocytes of transition dairy cows during hot season. J Dairy Sci. 2002;85:2173–9.CrossRefPubMed

28.

Bhat S, Rao G, Murthy KD, Bhat PG. Seasonal variations in markers of stress and oxidative stress in rats. Indian J Clin Biochem. 2008;23:191–4.CrossRefPubMedPubMedCentral

29.

Chigerwe M, Beck AD, Kim SS, Coons DM. Comparison of plasma oxidative status biomarkers in neonatal dairy calves during summer and fall seasons. J Vet Sci Technol. 2013;S11:006.

30.

Mujkosová J, Ferko M, Humeník P, Waczulíková I, Ziegelhoffer A. Seasonal variations in properties of healthy and diabetic rat heart mitochondria: Mg2 + −ATPase activity, content of conjugated dienes and membrane fluidity. Physiol Res. 2008;57 Suppl 2:S75–82.PubMed

31.

Pomerleau CS, Pomerleau OF, Snedecor SM, Mehringer AM. Defining a never-smoker: results from the nonsmokers survey. Addict Behav. 2004;29:1149–54.CrossRefPubMed

32.

Wasowicz W, Neve J, Peretz A, Nève J. Optimized steps in fluorometric determination of thiobarbituric acid-reactive substances in serum: importance of extraction pH and influence of sample preservation and storage. Clin Chem. 1993;39:2522–6.PubMed

33.

Beauchamp C, Fridovich I. Superoxide dismutase: improved assays and an assay applicable to acrylamide gels. Anal Biochem. 1971;44:276–87.CrossRefPubMed

34.

Paglia DE, Valentine WN. Studies on the quantitative and qualitative characterization of erythrocyte glutathione peroxidase. J Lab Clin Med. 1967;70(0022–2143 (Print)):158–69.PubMed

35.

Sunderman Jr FW, Nomoto S. Measurement of human serum ceruloplasmin by its p-phenylenediamine oxidase activity. Clin Chem. 1970;16:903–10.PubMed

36.

Gjolstad M, Thorud S, Molander P. Occupational exposure to airborne solvents during nail sculpturing. J Environ Monit. 2006;8:537–42.CrossRefPubMed

37.

Møller P, Knudsen LE, Loft S. The Comet assay as a rapid test in biomonitoring occupational exposure to DNA-damaging agents and effect of confounding factors 1. Cancer Epidemiol Biomarker Prev. 2000;9:1005–15.

38.

Miura T, Muraoka S, Fujimoto Y. Temperature-dependent lipid peroxidation of rat brain homogenate. Res Commun Mol Pathol Pharmacol. 1998;100:117–28.PubMed

39.

Rossner P, Svecova V, Milcova A, Lnenickova Z, Solansky I, Sram RJ. Seasonal variability of oxidative stress markers in city bus drivers. Part I. Oxidative damage to DNA. Mutat Res. 2008;642:14–20.CrossRefPubMed

40.

Rossner P, Svecova V, Milcova A, Lnenickova Z, Solansky I, Sram RJ. Seasonal variability of oxidative stress markers in city bus drivers. Part II. Oxidative damage to lipids and proteins. Mutat Res. 2008;642:21–7.CrossRefPubMed

41.

Yang JH, Han D-H. Occupational exposure of nail technicians to airborne chemicals and biological monitoring. J Korean Soc Occup Environ Hyg. 2010;20:53–62.

42.

Goldin LJ, Ansher L, Berlin A, Cheng J, Kanopkin D, Khazan A, Kisivuli M, Lortie M, Bunker Peterson E, Pohl L, Porter S, Zeng V, Skogstrom T, Fragala MA, Myatt TA, Stewart JH, Allen JG. Indoor air quality survey of nail salons in Boston. J Immigr Minor Health. 2013;8:508–14.

43.

Alaves VM, Sleeth DK, Thiese MS, Larson RR. Characterization of indoor air contaminants in a randomly selected set of commercial nail salons in Salt Lake County, Utah, USA. Int J Environ Health Res. 2013;23:419–33.CrossRefPubMed

44.

Wallace L, Emmerich S, Howard-Reed C. Continuous measurements of air change rates in an occupied house for 1 year: effect of temperature, winds, fans and windows. J Expo Anal Environ Epidemiol. 2002;12:296–306.CrossRefPubMed

45.

Comporti M, Signorini C, Leoncini S, Gardi C, Ciccoli L, Giardini A, Vecchio D, Arezzini B. Ethanol-induced oxidative stress: basic knowledge. Genes Nutr. 2010;5:101–9.CrossRefPubMed

46.

Hong E, Lee E, Kim Y, Oh E, Kim YW, Moon KW, Choi JW, Lee J, Roh J. The correlations between oxidative stress markers and indoor volatile organic compounds among the general population in Ansan and Incheon cities, Korea. Toxicol Environ Health Sci. 2009;1:37–48.CrossRef

Title: Does the Low-level occupational exposure to volatile organic compounds alter the seasonal variation of selected markers of oxidative stress? A case–control study in nail technicians
Authors: Peter Grešner
Radosław Świercz
Magdalena Beata Król
Ewa Twardowska
Jolanta Gromadzińska
Wojciech Wąsowicz
Publication date: 01-12-2016
Publisher: BioMed Central
Published in: Journal of Occupational Medicine and Toxicology / Issue 1/2016
Electronic ISSN: 1745-6673
DOI: https://doi.org/10.1186/s12995-016-0125-6

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Springer Medicine

Does the Low-level occupational exposure to volatile organic compounds alter the seasonal variation of selected markers of oxidative stress? A case–control study in nail technicians

Abstract

Background

Methods

Results

Conclusion

Keynote webinar | Spotlight on medication adherence

Springer Medicine

Abstract

Background

Methods

Results

Conclusion

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