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Archives of Gynecology and Obstetrics

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01-12-2014 | Gynecologic Endocrinology and Reproductive Medicine

Do follicular fluid gelatinase levels affect fertilization rates and oocyte quality?

Authors: Erdal Bilen, Esra Nur Tola, Baha Oral, Duygu Kumbul Doguç, İlker Günyeli, Seyit Ali Köse, Ilter İlhan

Published in: Archives of Gynecology and Obstetrics | Issue 6/2014

Abstract

Background

Matrix metalloproteinase-2 and -9, known as gelatinases, are considered to be essential for tissue remodelling during the reproductive process. However, their role in reproduction is unclear.

Aims

In the present study, we aimed to investigate the relationship between follicular fluid gelatinase levels and oocyte quality and fertilization, and to compare the activities of gelatinase levels with different drug stimulation protocols.

Methods

We evaluated 60 women with unexplained infertility who underwent in vitro fertilization (IVF) treatment. Thirty patients underwent a gonadotropin-releasing hormone (GnRH) agonist protocol and 30 underwent a GnRH antagonist protocol. Follicular fluid was obtained during oocyte retrieval. Oocyte quality was determined using light microscopy, and oocytes were considered to be fertilized when two pronuclei were present. Gelatinase activities were measured using commercial enzyme-linked immunosorbent assay kits. The study was partially supported by the Scientific Research Unit of Suleyman Demirel University (Protocol Number: 3620-TU1-13), and all procedures were conducted with the approval of the Suleyman Demirel University Local Ethics Board. Statistical analyses of the data were performed using the independent t test, Fisher exact test, Mann–Whitney U test, one-way ANOVA, and posthoc least significant difference.

Results

Follicular fluid gelatinase levels were significantly higher for agonist drug administration (p = 0.001), and a positive correlation was observed between matrix metalloproteinase-9 levels and oocyte grade (p = 0.01). Moreover, a positive relationship between matrix metalloproteinase-9 levels and fertilization was observed (p = 0.02).

Conclusion

Follicular fluid gelatinase activities, particularly MMP-9 activity, might be a predictor of oocyte quality and IVF success.

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Title: Do follicular fluid gelatinase levels affect fertilization rates and oocyte quality?
Authors: Erdal Bilen
Esra Nur Tola
Baha Oral
Duygu Kumbul Doguç
İlker Günyeli
Seyit Ali Köse
Ilter İlhan
Publication date: 01-12-2014
Publisher: Springer Berlin Heidelberg
Published in: Archives of Gynecology and Obstetrics / Issue 6/2014
Print ISSN: 0932-0067
Electronic ISSN: 1432-0711
DOI: https://doi.org/10.1007/s00404-014-3370-x

Keynote webinar | Spotlight on medication adherence

Springer Medicine

Do follicular fluid gelatinase levels affect fertilization rates and oocyte quality?

Abstract

Background

Aims

Methods

Results

Conclusion

Keynote webinar | Spotlight on medication adherence

Springer Medicine

Abstract

Background

Aims

Methods

Results

Conclusion

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