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Archives of Virology
Published in: Archives of Virology 8/2011

01-08-2011 | Original Article

DNA genome of spontaneously occurring deletion mutants of herpes simplex virus type 1 lacking one copy of the inverted repeat sequences of the L component

Authors: Kenichi Umene, Yasuyuki Fukumaki

Published in: Archives of Virology | Issue 8/2011

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Abstract

Three non-engineered, spontaneously occurring herpes simplex virus type 1 (HSV-1) mutants (GN52, GN82, and GN91) that have a deletion of approximately 10 kbp (including a part of the UL55 gene, the entire UL56 gene, and one copy of the inverted repeat sequences of the L component (RL)) and retain the a sequence were isolated. The yields of the mutants at 24 h post-adsorption in cultured cells were comparable to that of an HSV-1 isolate (GN28) without the deletion. Although the three mutants lost one copy of RL, the L component in replicative intermediates of the mutants inverted. DNA replicative intermediates of the three mutants were flanked by the L component, like those of GN28. The three mutants were generated through recombination involving regions around the authentic cleavage site in the a sequence, suggesting an important role of the a sequence in the diversification of herpesviruses.
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Metadata
Title
DNA genome of spontaneously occurring deletion mutants of herpes simplex virus type 1 lacking one copy of the inverted repeat sequences of the L component
Authors
Kenichi Umene
Yasuyuki Fukumaki
Publication date
01-08-2011
Publisher
Springer Vienna
Published in
Archives of Virology / Issue 8/2011
Print ISSN: 0304-8608
Electronic ISSN: 1432-8798
DOI
https://doi.org/10.1007/s00705-011-0983-2

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