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01-09-2021 | Short Communication

DNA-based eyelid trait prediction in Chinese Han population

Authors: Qian Wang, Bo Jin, Fan Liu, Zhilong Li, Yu Tan, Weibo Liang, Feijun Huang

Published in: International Journal of Legal Medicine | Issue 5/2021

Abstract

The eyelid folding represents one of the most distinguishing features of East Asian faces, involving the absence or presence of the eyelid crease, i.e., single vs. double eyelid. Recently, a genome-wide association study (GWAS) identified two SNPs (rs12570134 and rs1415425) showing genome-wide significant association with the double eyelid phenotype in Japanese. Here we report a confirmatory study in 697 Chinese individuals of exclusively Han origin. Only rs1415425 was statistically significant (P-value = 0.011), and the allele effect was on the same direction with that reported in Japanese. This SNP combined with gender and age explained 10.0% of the total variation in eyelid folding. DNA-based prediction model for the eyelid trait was developed and evaluated using logistic regression. The model showed mild to moderate predictive capacity (AUC = 0.69, sensitivity = 63%, and specificity = 70%). We further selected six additional SNPs by massive parallel sequencing of 19 candidate genes in 24 samples, and one SNP rs2761882 was statistically significant (P-value = 0.027). All predictors including these two SNPs (rs1415425 and rs2761882), gender, and age explained 11.2% of the total variation. The combined prediction model obtained an improved predictive capacity (AUC = 0.72, sensitivity = 62%, and specificity = 66%). Our study thus provided a confirmation of previous GWAS findings and a DNA-based prediction of the eyelid trait in Chinese Han individuals. This model may add value to forensic DNA phenotyping applications considering gender and age can be separately inferred from genetic and epigenetic markers. To further improve the prediction accuracy, future studies should focus on identifying more informative SNPs by large GWASs in East Asian populations.

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Kiferle L, Orsucci D, Mancuso M, Lo Gerfo A, Petrozzi L, Siciliano G, Ceravolo R, Bonuccelli U (2013) Twinkle mutation in an Italian family with external progressive ophthalmoplegia and Parkinsonism: a case report and an update on the state of art. Neurosci Lett 556:1–4. https://doi.org/10.1016/j.neulet.2013.09.034CrossRefPubMed

Kayser M (2015) Forensic DNA phenotyping: predicting human appearance from crime scene material for investigative purposes. Forensic Sci Int Genet 18:33–48. https://doi.org/10.1016/j.fsigen.2015.02.003CrossRefPubMed

Endo C, Johnson TA, Morino R, Nakazono K, Kamitsuji S, Akita M, Kawajiri M, Yamasaki T, Kami A, Hoshi Y, Tada A, Ishikawa K, Hine M, Kobayashi M, Kurume N, Tsunemi Y, Kamatani N, Kawashima M (2018) Genome-wide association study in Japanese females identifies fifteen novel skin-related trait associations. Sci Rep 8(1):8974. https://doi.org/10.1038/s41598-018-27145-2CrossRefPubMedPubMedCentral

Visconti A, Duffy DL, Liu F, Zhu G, Wu W, Chen Y, Hysi PG, Zeng C, Sanna M, Iles MM, Kanetsky PA, Demenais F, Hamer MA, Uitterlinden AG, Ikram MA, Nijsten T, Martin NG, Kayser M, Spector TD, Han J, Bataille V, Falchi M (2018) Genome-wide association study in 176,678 Europeans reveals genetic loci for tanning response to sun exposure. Nat Commun 9(1):1684. https://doi.org/10.1038/s41467-018-04086-yCrossRefPubMedPubMedCentral

Breslin K, Wills B, Ralf A, Ventayol Garcia M, Kukla-Bartoszek M, Pospiech E, Freire-Aradas A, Xavier C, Ingold S, de La Puente M, van der Gaag KJ, Herrick N, Haas C, Parson W, Phillips C, Sijen T, Branicki W, Walsh S, Kayser M (2019) Hirisplex-S system for eye, hair, and skin color prediction from DNA: massively parallel sequencing solutions for two common forensically used platforms. Forensic Sci Int Genet 43:102152. https://doi.org/10.1016/j.fsigen.2019.102152CrossRefPubMed

Pospiech E, Karlowska-Pik J, Marcinska M, Abidi S, Andersen JD, MVD B, Carracedo A, Eduardoff M, Freire-Aradas A, Morling N, Sijen T, Skowron M, Sochtig J, Syndercombe-Court D, Weiler N, Schneider PM, Ballard D, Borsting C, Parson W, Phillips C, Branicki W, Consortium EU-N (2015) Evaluation of the predictive capacity of DNA variants associated with straight hair in Europeans. Forensic Sci Int Genet 19:280–288. https://doi.org/10.1016/j.fsigen.2015.09.004CrossRefPubMed

Park JI (2000) Modified Z-Epicanthoplasty in the Asian eyelid. Arch Facial Plast Surg 2:43–47.

Chen WPD (2006) Comparative anatomy of the eyelids. Asian Blepharoplasty and the Eyelid Crease (Second Edition):3–22

Brinkley JF, Fisher S, Harris MP, Holmes G, Hooper JE, Jabs EW, Jones KL, Kesselman C, Klein OD, Maas RL, Marazita ML, Selleri L, Spritz RA, van Bakel H, Visel A, Williams TJ, Wysocka J, FaceBase C, Chai Y (2016) The Facebase Consortium: a comprehensive resource for craniofacial researchers. Development 143(14):2677-2688. https://doi.org/10.1242/dev.135434

10.

Eker HK, Derinkuyu BE, Ünal S, Masliah-Planchon J, Drunat S, Verloes A (2014) Cerebro-fronto-facial syndrome type 3 with polymicrogyria: a clinical presentation of Baraitser–Winter syndrome. Eur J Med Genet 57(1):32–36. https://doi.org/10.1016/j.ejmg.2013.10.005CrossRefPubMed

11.

Di Donato N, Rump A, Koenig R, Der Kaloustian VM, Halal F, Sonntag K, Krause C, Hackmann K, Hahn G, Schrock E, Verloes A (2013) Severe forms of Baraitser–Winter syndrome are caused by Actb mutations rather than Actg1 mutations. Eur J Hum Genet 22(2):179–183. https://doi.org/10.1038/ejhg.2013.130CrossRefPubMedPubMedCentral

12.

Ullah A, Umair M, U EK, Shahzad S, Basit S, Ahmad W (2018) Exome sequencing revealed a novel nonsense variant in Alx3 gene underlying frontorhiny. J Hum Genet 63(1):97–100. https://doi.org/10.1038/s10038-017-0358-yCrossRefPubMed

13.

Twigg SR, Versnel SL, Nurnberg G, Lees MM, Bhat M, Hammond P, Hennekam RC, Hoogeboom AJ, Hurst JA, Johnson D, Robinson AA, Scambler PJ, Gerrelli D, Nurnberg P, Mathijssen IM, Wilkie AO (2009) Frontorhiny, a distinctive presentation of frontonasal dysplasia caused by recessive mutations in the Alx3 homeobox gene. Am J Hum Genet 84(5):698–705. https://doi.org/10.1016/j.ajhg.2009.04.009CrossRefPubMedPubMedCentral

14.

de Iongh RULF, Overbeek PA, Schneider MD, Joya J, Hardeman ED, McAvoy JW (2001) Requirement for Tgfβ receptor signaling during terminal lens fiber differentiation. Development 128(20):3995–4010CrossRef

15.

Blackburn PR, Zepeda-Mendoza CJ, Kruisselbrink TM, Schimmenti LA, Garcia-Minaur S, Palomares M, Nevado J, Mori MA, Le Meur G, Klee EW, Le Caignec C, Lapunzina P, Isidor B, Babovic-Vuksanovic D (2019) Variable expressivity of syndromic Bmp4-related eye, brain, and digital anomalies: a review of the literature and description of three new cases. Eur J Hum Genet 27(9):1379–1388. https://doi.org/10.1038/s41431-019-0423-4

16.

Morino H, Pierce SB, Matsuda Y, Walsh T, Ohsawa R, Newby M, Hiraki-Kamon K, Kuramochi M, Lee MK, Klevit RE, Martin A, Maruyama H, King MC, Kawakami H (2014) Mutations in twinkle primase-helicase cause Perrault syndrome with neurologic features. Neurology 83(22):2054–2061. https://doi.org/10.1212/Wnl.0000000000001036CrossRefPubMedPubMedCentral

17.

Mendoza-Londono R, Chitayat D, Kahr WH, Hinek A, Blaser S, Dupuis L, Goh E, Badilla-Porras R, Howard A, Mittaz L, Superti-Furga A, Unger S, Nishimura G, Bonafe L (2012) Extracellular matrix and platelet function in patients with musculocontractural Ehlers-Danlos syndrome caused by mutations in the Chst14 gene. Am J Med Genet A 158a(6):1344–1354. https://doi.org/10.1002/ajmg.a.35339CrossRefPubMed

18.

Malfait F, Syx D, Vlummens P, Symoens S, Nampoothiri S, Hermanns-Lê T, Van Laer L, De Paepe A (2010) Musculocontractural Ehlers-Danlos syndrome (former Eds type Vib) and adducted thumb clubfoot syndrome (Atcs) represent a single clinical entity caused by mutations in the dermatan-4-sulfotransferase 1 encoding Chst14 gene. Hum Mutat 31(11):1233–1239. https://doi.org/10.1002/humu.21355

19.

Straten C, Butow K-W (2013) Gene P63: in ectrodactyly-ectodermal dysplasia clefting, ankyloblepharon-ectodermal dysplasia, Rapp-Hodgkin syndrome. Ann Maxillofac Surg 3(1):58. https://doi.org/10.4103/2231-0746.110085CrossRefPubMedPubMedCentral

20.

Brunner HG, Hamel BCJ, van Bokhoven H (2002) The P63 gene in Eec and other syndromes. J Med Genet 39(6):377–381. https://doi.org/10.1136/Jmg.39.6.377

21.

Ojeda AF, Munjaal RP, Lwigale PY (2013) Expression of Cxcl12 and Cxcl14 during eye development in chick and mouse. Gene Expr Patterns 13(8):303–310. https://doi.org/10.1016/j.gep.2013.05.006CrossRefPubMed

22.

Ojeda AF, Munjaal RP, Lwigale PY (2017) Knockdown of Cxcl14 disrupts neurovascular patterning during ocular development. Dev Biol 423(1):77–91. https://doi.org/10.1016/j.ydbio.2017.01.008CrossRefPubMedPubMedCentral

23.

Griffin JN, Compagnucci C, Hu D, Fish J, Klein O, Marcucio R, Depew MJ (2013) Fgf8 dosage determines midfacial integration and polarity within the nasal and optic capsules. Dev Biol 374(1):185–197. https://doi.org/10.1016/j.ydbio.2012.11.014CrossRefPubMed

24.

Green RM, Feng W, Phang T, Fish JL, Li H, Spritz RA, Marcucio RS, Hooper J, Jamniczky H, Hallgrimsson B, Williams T (2015) Tfap2a-dependent changes in mouse facial morphology result in clefting that can be ameliorated by a reduction in Fgf8 gene dosage. Dis Model Mech 8(1):31–43. https://doi.org/10.1242/dmm.017616CrossRefPubMed

25.

Smith RS, Zabaleta A, Kume T, Savinova OV, Kidson SH, Martin JE, Nishimura DY, Alward WL, Hogan BL, John SW (2000) Haploinsufficiency of the transcription factors Foxc1 and Foxc2 results in aberrant ocular development. Hum Mol Genet 9(7):1021–1032. https://doi.org/10.1093/hmg/9.7.1021CrossRefPubMed

26.

Tumer Z, Bach-Holm D (2009) Axenfeld-Rieger syndrome and spectrum of Pitx2 and Foxc1 mutations. Eur J Hum Genet 17(12):1527–1539. https://doi.org/10.1038/ejhg.2009.93CrossRefPubMedPubMedCentral

27.

Reis LM, Tyler RC, Volkmann Kloss BA, Schilter KF, Levin AV, Lowry RB, Zwijnenburg PJ, Stroh E, Broeckel U, Murray JC, Semina EV (2012) Pitx2 and Foxc1 spectrum of mutations in ocular syndromes. Eur J Hum Genet 20(12):1224–1233. https://doi.org/10.1038/ejhg.2012.80CrossRefPubMedPubMedCentral

28.

Huang J, Dattilo LK, Rajagopal R, Liu Y, Kaartinen V, Mishina Y, Deng CX, Umans L, Zwijsen A, Roberts AB, Beebe DC (2009) Fgf-regulated Bmp signaling is required for eyelid closure and to specify conjunctival epithelial cell fate development. 136(10):1741–1750. https://doi.org/10.1242/dev.034082

29.

Herrera E, Marcus R, Li S, Williams SE, Erskine L, Lai E, Mason C (2004) Foxd1 is required for proper formation of the optic chiasm. Development 131(22):5727–5739. https://doi.org/10.1242/dev.01431CrossRefPubMed

30.

Carreres MI, Escalante A, Murillo B, Chauvin G, Gaspar P, Vegar C, Herrera E (2011) Transcription factor Foxd1 is required for the specification of the temporal retina in mammals. J Neurosci 31(15):5673–5681. https://doi.org/10.1523/JNEUROSCI.0394-11.2011CrossRefPubMedPubMedCentral

31.

Wang T, Tamakoshi T, Uezato T, Shu F, Kanzaki-Kato N, Fu Y, Koseki H, Yoshida N, Sugiyama T, Miura N (2003) Forkhead transcription factor Foxf2 (Lun)-deficient mice exhibit abnormal development of secondary palate. Dev Biol 259(1):83–94. https://doi.org/10.1016/s0012-1606(03)00176-3CrossRefPubMed

32.

Bu LX, Chen QQ, Wang H, Zhang TX, Hetmanski JB, Schwender H, Parker M, Chou YHW, Yeow V, Chong SS, Zhang B, Jabs EW, Scott AF, Beaty TH (2015) Novel evidence of association with nonsyndromic cleft lip with or without cleft palate was shown for single nucleotide polymorphisms in Foxf2 gene in an Asian population. Birth Defects Research Part a-Clinical and Molecular. Teratology 103(10):857–862. https://doi.org/10.1002/bdra.23413CrossRef

33.

Nuovo S, Passeri M, Di Benedetto E, Calanchini M, Meldolesi I, Di Giacomo MC, Petruzzi D, Piemontese MR, Zelante L, Sangiuolo F, Novelli G, Fabbri A, Brancati F (2016) Characterization of endocrine features and genotype-phenotypes correlations in blepharophimosis-ptosis-epicanthus inversus syndrome type 1. J Endocrinol Investig 39(2):227–233. https://doi.org/10.1007/s40618-015-0334-3CrossRef

34.

Crisponi L, Deiana M, Loi A, Chiappe F, Uda M, Amati P, Bisceglia L, Zelante L, Nagaraja R, Porcu S, Ristaldi MS, Marzella R, Rocchi M, Nicolino M, Lienhardt-Roussie A, Nivelon A, Verloes A, Schlessinger D, Gasparini P, Bonneau D, Cao A, Pilia G (2001) The putative forkhead transcription factor Foxl2 is mutated in blepharophimosis/ptosis/epicanthus inversus syndrome. Nat Genet 27(2):159–166. https://doi.org/10.1038/84781CrossRefPubMed

35.

Mine N, Iwamoto R, Mekada E (2005) Hb-Egf promotes epithelial cell migration in eyelid development. Development 132(19):4317–4326. https://doi.org/10.1242/dev.02030CrossRefPubMed

36.

Brown CW, Li L, Houston-Hawkins DE, Matzuk MM (2003) Activins are critical modulators of growth and survival. Mol Endocrinol 17(12):2404–2417. https://doi.org/10.1210/me.2003-0051CrossRefPubMed

37.

Zenz R, Scheuch H, Martin P, Frank C, Eferl R, Kenner L, Sibilia M, Wagner EF (2003) C-Jun regulates eyelid closure and skin tumor development through Egfr signaling. Dev Cell 4(6):879–889. https://doi.org/10.1016/s1534-5807(03)00161-8CrossRefPubMed

38.

Meng QH, Mongan M, Carreira V, Kurita H, Liu CY, Kao WWY, Xia Y (2014) Eyelid closure in embryogenesis is required for ocular adnexa development. Invest Ophthalmol Vis Sci 55(11):7652–7661. https://doi.org/10.1167/iovs.14-15155CrossRefPubMedPubMedCentral

39.

Chakravarti S, Petroll WM, Hassell JR, Jester JV, Lass JH, Paul J, Birk DE (2000) Corneal opacity in lumican-null mice: defects in collagen fibril structure and packing in the posterior stroma. Invest Ophthalmol Vis Sci 41(11):3365–3373PubMed

40.

Song J, Lee YG, Houston J, Petroll WM, Chakravarti S, Cavanagh HD, Jester JV (2003) Neonatal corneal stromal development in the normal and lumican-deficient mouse. Invest Ophthalmol Vis Sci 44(2):548–557. https://doi.org/10.1167/iovs.02-0592CrossRefPubMed

41.

Joly S, Pernet V (2016) Sphingosine 1-phosphate receptor 1 is required for retinal ganglion cell survival after optic nerve trauma. J Neurochem 138(4):571–586. https://doi.org/10.1111/jnc.13701CrossRefPubMed

42.

Joly S, Dalkara D, Pernet V (2017) Sphingosine 1-phosphate receptor 1 modulates Cntf-induced axonal growth and neuroprotection in the mouse visual system. Neural Plast 2017:1–11. https://doi.org/10.1155/2017/6818970CrossRef

43.

Ohuchi H (2012) Wakayama symposium: epithelial-mesenchymal interactions in eyelid development. Ocul Surf 10(4):212–216. https://doi.org/10.1016/j.jtos.2012.07.005CrossRefPubMed

44.

Tao H (2005) A dual role of Fgf10 in proliferation and coordinated migration of epithelial leading edge cells during mouse eyelid development. Development 132(14):3217–3230. https://doi.org/10.1242/dev.01892CrossRefPubMed

45.

Garcez RC, Le Douarin NM, Creuzet SE (2014) Combinatorial activity of Six1-2-4 genes in cephalic neural crest cells controls craniofacial and brain development. Cell Mol Life Sci 71(11):2149–2164. https://doi.org/10.1007/s00018-013-1477-z

46.

Kawakami K, Sato S, Ozaki H, Ikeda K (2000) Six family genes-structure and function as transcription factors and their roles in development. Bioessays 22(7):616–626CrossRef

47.

Gibson G, Liu F, van der Lijn F, Schurmann C, Zhu G, Chakravarty MM, Hysi PG, Wollstein A, Lao O, de Bruijne M, Ikram MA, van der Lugt A, Rivadeneira F, Uitterlinden AG, Hofman A, Niessen WJ, Homuth G, de Zubicaray G, McMahon KL, Thompson PM, Daboul A, Puls R, Hegenscheid K, Bevan L, Pausova Z, Medland SE, Montgomery GW, Wright MJ, Wicking C, Boehringer S, Spector TD, Paus T, Martin NG, Biffar R, Kayser M (2012) A genome-wide association study identifies five loci influencing facial morphology in Europeans. PLoS Genet 8(9):e1002932. https://doi.org/10.1371/journal.pgen.1002932CrossRef

48.

Chen Y, Yang F, Zheng H, Zhou J, Zhu G, Hu P, Wu W (2016) Clinical and genetic investigation of families with type Ii Waardenburg syndrome. Mol Med Rep 13(3):1983–1988. https://doi.org/10.3892/mmr.2016.4774CrossRefPubMedPubMedCentral

49.

Kapoor S, Bindu PS, Taly AB, Sinha S, Gayathri N, Rani SV, Chandak GR, Kumar A (2012) Genetic analysis of an Indian family with members affected with Waardenburg syndrome and Duchenne muscular dystrophy. Mol Vis 18(211-14):2022–2032PubMedPubMedCentral

50.

McNeill B, Perez-Iratxeta C, Mazerolle C, Furimsky M, Mishina Y, Andrade-Navarro MA, Wallace VA (2012) Comparative genomics identification of a novel set of temporally regulated Hedgehog target genes in the retina. Mol Cell Neurosci 49(3):333–340. https://doi.org/10.1016/j.mcn.2011.12.008CrossRefPubMed

51.

Guth SI, Bosl MR, Sock E, Wegner M (2010) Evolutionary conserved sequence elements with embryonic enhancer activity in the vicinity of the mammalian Sox8 gene. Int J Biochem Cell Biol 42(3):465–471. https://doi.org/10.1016/j.biocel.2009.07.008CrossRefPubMed

52.

Kuracha MR, Siefker E, Licht JD, Govindarajan V (2013) Spry1 and Spry2 are necessary for eyelid closure. Dev Biol 383(2):227–238. https://doi.org/10.1016/j.ydbio.2013.09.014CrossRefPubMed

53.

Kuracha MR, Burgess D, Siefker E, Cooper JT, Licht JD, Robinson ML, Govindarajan V (2011) Spry1 and Spry2 are necessary for lens vesicle separation and corneal differentiation. Invest Ophthalmol Vis Sci 52(9):6887–6897. https://doi.org/10.1167/iovs.11-7531CrossRefPubMedPubMedCentral

54.

Gestri G, Osborne RJ, Wyatt AW, Gerrelli D, Gribble S, Stewart H, Fryer A, Bunyan DJ, Prescott K, Collin JRO, Fitzgerald T, Robinson D, Carter NP, Wilson SW, Ragge NK (2009) Reduced Tfap2a function causes variable optic fissure closure and retinal defects and sensitizes eye development to mutations in other morphogenetic regulators. Hum Genet 126(6):791–803. https://doi.org/10.1007/s00439-009-0730-xCrossRefPubMedPubMedCentral

55.

Milunsky JM, Maher TA, Zhao G, Roberts AE, Stalker HJ, Zori RT, Burch MN, Clemens M, Mulliken JB, Smith R, Lin AE (2008) Tfap2a mutations result in branchio-oculo-facial syndrome. Am J Hum Genet 82(5):1171–1177. https://doi.org/10.1016/j.ajhg.2008.03.005CrossRefPubMedPubMedCentral

56.

Zhao S, Overbeek PA (2001) Elevated Tgfβ signaling inhibits ocular vascular development. Dev Biol 237(1):45–53. https://doi.org/10.1006/dbio.2001.0360CrossRefPubMed

57.

Saika S (2005) Tgfβ pathobiology in the eye. Lab Investig 86(2):106–115. https://doi.org/10.1038/labinvest.3700375CrossRef

58.

Walsh S, Wollstein A, Liu F, Chakravarthy U, Rahu M, Seland JH, Soubrane G, Tomazzoli L, Topouzis F, Vingerling JR, Vioque J, Fletcher AE, Ballantyne KN, Kayser M (2012) DNA-based eye colour prediction across Europe with the Irisplex system. Forensic Sci Int Genet 6(3):330–340. https://doi.org/10.1016/j.fsigen.2011.07.009CrossRefPubMed

59.

Mupas-Uy J, Kitaguchi Y, Takahashi Y, Kakizaki H (2017) Age-related eyelid changes. J Cosmet Med 1(1):16–24. https://doi.org/10.25056/jcm.2017.1.1.16CrossRef

60.

Martinez-Fernandez ML, Bermejo-Sanchez E, Fernandez B, MacDonald A, Fernandez-Toral J, Martinez-Frias ML (2014) Haploinsufficiency of Bmp4 gene may be the underlying cause of Frias syndrome. Am J Med Genet A 164A(2):338–345. https://doi.org/10.1002/ajmg.a.36224CrossRefPubMed

61.

Chen Q, Wang H, Hetmanski JB, Zhang T, Ruczinski I, Schwender H, Liang KY, Fallin MD, Redett RJ, Raymond GV, Wu Chou YH, Chen PK, Yeow V, Chong SS, Cheah FS, Jabs EW, Scott AF, Beaty TH (2012) Bmp4 was associated with Nscl/P in an Asian population. PLoS One 7(4):e35347. https://doi.org/10.1371/journal.pone.0035347CrossRefPubMedPubMedCentral

62.

Suazo J, Santos JL, Jara L, Blanco R (2010) Association between bone morphogenetic protein 4 gene polymorphisms with nonsyndromic cleft lip with or without cleft palate in a Chilean population. DNA Cell Biol 29(2):59–64. https://doi.org/10.1089/dna.2009.0944CrossRefPubMed

63.

Zawislak A, Wozniak K, Jakubowska A, Lubinski J, Kawala B, Znamirowska-Bajowska A (2014) Polymorphic variants in Vax1 gene (Rs7078160) and Bmp4 gene (Rs762642) and the risk of non-syndromic orofacial clefts in the Polish population. Dev Period Med 18(1):16–22PubMed

64.

Parson W (2018) Age estimation with DNA: from forensic DNA fingerprinting to forensic (epi)genomics: a mini-review. Gerontology 64(4):326–332. https://doi.org/10.1159/000486239CrossRefPubMed

65.

Vidaki A, Ballard D, Aliferi A, Miller TH, Barron LP, Syndercombe Court D (2017) DNA methylation-based forensic age prediction using artificial neural networks and next generation sequencing. Forensic Sci Int Genet 28:225–236. https://doi.org/10.1016/j.fsigen.2017.02.009CrossRefPubMedPubMedCentral

Title: DNA-based eyelid trait prediction in Chinese Han population
Authors: Qian Wang
Bo Jin
Fan Liu
Zhilong Li
Yu Tan
Weibo Liang
Feijun Huang
Publication date: 01-09-2021
Publisher: Springer Berlin Heidelberg
Published in: International Journal of Legal Medicine / Issue 5/2021
Print ISSN: 0937-9827
Electronic ISSN: 1437-1596
DOI: https://doi.org/10.1007/s00414-021-02570-7

Keynote webinar | Spotlight on medication adherence

Springer Medicine

DNA-based eyelid trait prediction in Chinese Han population

Abstract

Keynote webinar | Spotlight on medication adherence

Springer Medicine

Abstract

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