Skip to main content
Key Specialties
Cardiology Diabetology Endocrinology Gastroenterology Geriatrics and Gerontology Gynecology and Obstetrics Hematology Infectious Disease Internal Medicine Nephrology Neurology Oncology Pediatrics Respiratory Medicine Rheumatology Surgery
Congress Coverage
2024 ACC Congress 2023 ESMO Congress 2023 EASD Congress 2023 ERS Congress 2023 ESC Congress
Clinical Collections
Advances in Alzheimer’s

At a glance: The STEP trials

A round-up of the STEP phase 3 clinical trials evaluating semaglutide for weight loss in people with overweight or obesity.
ADVANCED SEARCH
Log in
Top
Chinese Medicine
Published in: Chinese Medicine 1/2018

Open Access 01-12-2018 | Research

Diversity and composition of bacterial endophytes among plant parts of Panax notoginseng

Authors: Linlin Dong, Ruiyang Cheng, Lina Xiao, Fugang Wei, Guangfei Wei, Jiang Xu, Yong Wang, Xiaotong Guo, Zhongjian Chen, Shilin Chen

Published in: Chinese Medicine | Issue 1/2018

Login to get access

Abstract

Background

Bacterial endophytes are widespread inhabitants inside plant tissues that play crucial roles in plant growth and biotransformation. This study aimed to offer information for the exploitation of endophytes by analyzing the bacterial endophytes in different parts of Panax notoginseng.

Methods

We used high-throughput sequencing methods to analyze the diversity and composition of bacterial endophytes from different parts of P. notoginseng.

Results

A total of 174,761 classified sequences were obtained from the analysis of 16S ribosomal RNA in different parts of P. notoginseng. Its fibril displayed the highest diversity of bacterial endophytes. Principal coordinate analysis revealed that the compositions of the bacterial endophytes from aboveground parts (flower, leaf, and stem) differed from that of underground parts (root and fibril). The abundances of Conexibacter, Gemmatimonas, Holophaga, Luteolibacter, Methylophilus, Prosthecobacter, and Solirubrobacter were significantly higher in the aboveground parts than in the underground parts, whereas the abundances of Bradyrhizobium, Novosphingobium, Phenylobacterium, Sphingobium, and Steroidobacter were markedly lower in the aboveground parts.

Conclusions

Our results elucidated the comprehensive diversity and composition profiles of bacterial endophytes in different parts of 3-year-old P. notoginseng. Our data offered pivotal information to clarify the role of endophytes in the production of P. notoginseng and its important metabolites.
Appendix
Available only for authorised users
Literature
1.
Ng T. Pharmacological activity of sanchi ginseng (Panax notoginseng). J Pharm Pharmacol. 2006;58:1007–19.CrossRefPubMed
2.
Zhang H, Cheng Y. Solid-phase extraction and liquid chromatography-electrospray mass spectrometric analysis of saponins in a Chinese patent medicine of formulated Salvia miltiorrhizae and Panax notoginseng. J Pharmaceut Biomed. 2006;40:429–32.CrossRef
3.
Liu X, Wang L, Chen X, Deng X, Cao Y, Wang Q. Simultaneous quantification of both triterpenoid and steroidal saponins in various Yunnan Baiyao preparations using HPLC-UV and HPLC-MS. J Sep Sci. 2008;31:3834–46.CrossRefPubMed
4.
Du Q, Jerz G, Waibel R, Winterhalter P. Isolation of dammarane saponins from Panax notoginseng by high-speed countercurrent chromatography. J Chromatogr A. 2003;1008:173–80.CrossRefPubMed
5.
Sun H, Yang Z, Ye Y. Structure and biological activity of protopanaxatriol-type saponins from the roots of Panax notoginseng. Int Immunopharmac. 2006;6:14–25.CrossRef
6.
Hu Z, You C, Zhang T. Discussing the obstacles caused by continuous notoginseng cropping. J Wenshan Univ. 2011;24:6–11.
7.
Liu L, Liu D, Jing H, Feng G, Zhang J, Wei M, et al. Overview on the mechanisms and control methods of sequential cropping obstacle of Panax notoginseng F. H. Chen. J Mountain Agri Biol. 2011;30:70–5.
8.
Ma C, Li S, Gu Z, Chen Y. Measures of integrated control of root rot complex of continuous cropping Panax notoginseng and their control efficacy. Acta Agri Shanghai. 2006;22:63–8.
9.
Larousse M, Rancurel C, Syska C, Palero F, Etienne C, Industri B, et al. Tomato root microbiota and Phytophthora parasitica-associated disease. Microbiome. 2017;5:56.CrossRefPubMedPubMedCentral
10.
Beckers B, De Beek MO, Weyens N, Boerjan W, Vangronsveld J. Structural variability and niche differentiation in the rhizosphere and endosphere bacterial microbiome of field-grown poplar trees. Microbiome. 2017;5:25.CrossRefPubMedPubMedCentral
11.
Hassan SED. Plant growth-promoting activities for bacterial and fungal endophytes isolated from medicinal plant of Teucrium polium L. J Adv Res. 2017;8:687–95.CrossRefPubMedPubMedCentral
12.
Singh M, Kumar A, Singh R, Pandey K. Endophytic bacteria: a new source of bioactive compounds. Biotech. 2017;7:315.
13.
Song X, Wu H, Yin Z, Lian M, Yin C. Endophytic bacteria isolated from Panax ginseng improved ginsenoside accumulation in adventitious ginseng root culture. Molecules. 2017;22:837.CrossRef
14.
Gao Y, Liu Q, Zang P, Li X, Ji Q, He Z, et al. An endophytic bacterium isolated from Panax ginseng C.A. Meyer enhances growth, reduces morbidity, and stimulates ginsenoside biosynthesis. Phytochem Lett. 2015;125:132–8.CrossRef
15.
Van Overbeek L, van Elsas J. Effects of plant genotype and growth stage on the structure of bacterial communities associated with potato (Solanum tuberosum L.). FEMS Microbiol Ecol. 2008;64:283–96.CrossRefPubMed
16.
Chowdhury E, Jeon J, Rim S, Park Y, Lee S, Bae H. Composition, diversity and bioactivity of culturable bacterial endophytes in mountain-cultivated ginseng in Korea. Sci Rep. 2017;7:10098.CrossRef
17.
Tan Y, Cui Y, Li H, Kuang A, Li X, Wei Y, et al. Diversity and composition of rhizospheric soil and root endogenous bacteria in Panax notoginseng during continuous cropping practices. J Basic Microb. 2016;9999:1–8.
18.
19.
Jin H, Yang X, Yan Z, Liu Q, Li X, Chen J, et al. Characterization of rhizosphere and endophytic bacterial communities from leaves, stems and roots of medicinal Stellera chamaejasme L. Syst Appl Microbiol. 2014;37:376–85.CrossRefPubMed
20.
Dong L, Xu J, Feng G, Li X, Chen S. Soil bacterial and fungal community dynamics in relation to Panax notoginseng death rate in a continuous cropping system. Sci Rep. 2016;6:31802.CrossRefPubMedPubMedCentral
21.
Dong L, Xu J, Zhang L, Yang J, Liao B, Li X, et al. High-throughput sequencing technology reveals that continuous cropping of American ginseng results in changes in the microbial community in arable soil. Chin Med. 2017;12:18.CrossRefPubMedPubMedCentral
22.
Wani Z, Ashraf N, Mohiuddin T, Riyaz-Ul-Hassan S. Plant-endophyte symbiosis, an ecological perspective. Appl Microbiol Biotechnol. 2015;99:2955–65.CrossRefPubMed
23.
24.
Checcucci A, Maida I, Bacci G, Ninno C, Bilia A, Biffi S, et al. Is the plant-associated microbiota of Thymus spp. adapted to plant essential oil? Res Microbiol. 2016;3:276–87.
25.
Akinsanya M, Goh J, Lim S, Ting A. Diversity, antimicrobial and antioxidant activities of culturable bacterial endophyte communities in Aloe vera. FEMS Microbiol Lett. 2015;362:184.CrossRef
26.
Jackson C, Randolph K, Osborn S, Tyler H. Culture dependent and independent analysis of bacterial communities associated with commercial salad leaf vegetables. BMC Microbiol. 2013;13:274.CrossRefPubMedPubMedCentral
27.
Dong L, Xu J, Zhang L, Cheng R, Wei G, Su H, Yang J, Qian J, Xu R, Chen S. Rhizospheric microbial communities are driven by Panax ginseng at different growth stages and biocontrol bacteria alleviates replanting mortality. Acta Pharm Sin B. 2018;8:272–82.CrossRefPubMedPubMedCentral
28.
Dong L, Xu J, Li Y, Fang H, Niu W, Li X, et al. Manipulation of microbial community in the rhizosphere alleviates the replanting issues in Panax ginseng. Soil Biol Biochem. 2018;125:64–74.CrossRef
29.
Fierer N, Jackson J, Vilgalys R, Jackson R. Assessment of soil microbial community structure by use of taxon-specific quantitative PCR assays. Appl Environ Microbiol. 2005;71:4117–20.CrossRefPubMedPubMedCentral
30.
Caporaso J, Bittinger K, Bushman F, DeSantis T, Andersen G, Knight R. PyNAST: a flexible tool for aligning sequences to a template alignment. Phylogenetics. 2010;26:266–7.
31.
Katherine R, Carl J, Angela K, Nicoletta R, Franck C, Alejandro E, et al. Habitat degradation impacts black howler monkey (Alouatta pigra) gastrointestinal microbiomes. ISME J. 2013;7:1344–53.CrossRef
32.
Schloss P, Westcott S, Ryabin T, Hall J, Hartmann M, Hollister E, et al. Introducing mothur: open-source, platform-independent, community-supported software for describing and comparing microbial communities. Appl Environ Micro. 2009;75:7537–41.CrossRef
33.
34.
Oksanen J, Blanchet F, Kindt R, Simpson G. Vegan: community ecology package. Version 2.0-10. J Stat Softw. 2011;48:1–21.
35.
36.
Mahanty T, Bhattacharjee S, Goswami M, Bhattacharyya P, Das B, Ghosh A, Tribedi P. Biofertilizers: a potential approach for sustainable agriculture development. Environ Sci Pollut Res Int. 2017;24:3315–35.CrossRefPubMed
37.
Berendsen RL, Pieterse CMJ, Bakker PHM. The rhizosphere microbiome and plant health. Trends Plant Sci. 2012;17:478–86.CrossRefPubMed
38.
Egamberdiyeva D. The effect of plant growth promoting bacteria on growth and nutrient uptake of maize in two different soils. Appl Soil Ecol. 2007;36:184–9.CrossRef
39.
Verma JP, Yadav J, Tiwari KN, Lavakush SV. Impact of plant growth promoting rhizobacteria on crop production. Int J Agri Res. 2010;5:954–83.CrossRef
40.
Shehata H, Lyons E, Jordan K, Raizada M. Relevance of in vitro agar based screens to characterize the anti-fungal activities of bacterial endophyte communities. BMC Microbiol. 2016;16:8.CrossRefPubMedPubMedCentral
41.
Li Y, Liu Q, Liu Y, Zhu J, Zhang Q. Endophytic bacterial diversity in roots of Typha angustifolia L. in the constructed Beijing Cuihu Wetland (China). Res Microbiol. 2011;162:124–31.CrossRefPubMed
42.
Katoch M, Phull S, Vaid S, Singh S. Diversity, Phylogeny, anticancer and antimicrobial potential of fungal endophytes associated with Monarda citriodora L. BMC Microbiol. 2017;17:44.CrossRefPubMedPubMedCentral
43.
Wei G, Dong L, Yang J, Zhang L, Xu J, Yang F, et al. Integrated metabolomic and transcriptomic analyses revealed the distribution of saponins in Panax notoginseng. Acta Pharm Sin B. 2018;8:458–65.CrossRefPubMedPubMedCentral
44.
Yang J, Dong L, Wei G, Hu H, Zhu G, Zhang J, et al. Identification and quality of Panax notoginseng and Panax vietnamensis var. fuscidicus through integrated DNA barcoding and HPLC. Chin Herbal Med. 2018;10:177–83.CrossRef
Metadata
Title
Diversity and composition of bacterial endophytes among plant parts of Panax notoginseng
Authors
Linlin Dong
Ruiyang Cheng
Lina Xiao
Fugang Wei
Guangfei Wei
Jiang Xu
Yong Wang
Xiaotong Guo
Zhongjian Chen
Shilin Chen
Publication date
01-12-2018
Publisher
BioMed Central
Published in
Chinese Medicine / Issue 1/2018
Electronic ISSN: 1749-8546
DOI
https://doi.org/10.1186/s13020-018-0198-5

Other articles of this Issue 1/2018

Chinese Medicine 1/2018 Go to the issue

Review

Internationalization of traditional Chinese medicine: current international market, internationalization challenges and prospective suggestions

Research

Rapid screening and identification of bioactive compounds specifically binding to beta 2-adrenoceptor from San-ao decoction using affinity magnetic fine particles coupled with high-performance liquid chromatography–mass spectrometry

Research

Paeoniflorin exerts neuroprotective effects by modulating the M1/M2 subset polarization of microglia/macrophages in the hippocampal CA1 region of vascular dementia rats via cannabinoid receptor 2

Research

Effective authentication of Placenta Hominis

Research

Siegesbeckia pubescens Makino inhibits Pam3CSK4-induced inflammation in RAW 264.7 macrophages through suppressing TLR1/TLR2-mediated NF-κB activation

Review

Approaches in studying the pharmacology of Chinese Medicine formulas: bottom-up, top-down—and meeting in the middle