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01-06-2003 | Original Article

Distribution of estrogen receptors α and β mRNA in mouse urogenital tissues and their expression after oophorectomy and estrogen replacement

Authors: Michael E. Carley, David J. Rickard, John B. Gebhart, Maurice J. Webb, Karl C. Podratz, Thomas C. Spelsberg

Published in: International Urogynecology Journal | Issue 2/2003

Abstract

Estrogen receptors are present in the urogenital tract. However, little is known about the quantitative distribution of the traditional estrogen receptor (ERα) mRNA and the recently identified ERβ mRNA. By quantitative reverse transcription polymerase chain reaction analysis, the distributions of ERα and ERβ mRNA in mouse urogenital tissues and their expression in selected urogenital tissues after oophorectomy, with or without estrogen replacement, were evaluated. ERα mRNA concentrations were higher in the ovary, oviduct, uterus and vagina than in the kidney, ureter or bladder (P<0.05); ERβ transcripts were highest in the ovary, oviduct and bladder (P<0.05). After oophorectomy and estrogen replacement, significant changes were identified in ERα and ERβ mRNA expression. ERα and ERβ mRNA are differentially expressed in mouse urogenital tissues. Oophorectomy and estrogen replacement affect estrogen receptors differently in the bladder, vagina and uterus. These results may explain some tissue-specific responses to estrogen and selective estrogen receptor modulators. Summary: The mRNA distributions of estrogen receptors α and β and their expression after oophorectomy, with or without estrogen replacement, differ in mouse urogenital tissues.

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Title: Distribution of estrogen receptors α and β mRNA in mouse urogenital tissues and their expression after oophorectomy and estrogen replacement
Authors: Michael E. Carley
David J. Rickard
John B. Gebhart
Maurice J. Webb
Karl C. Podratz
Thomas C. Spelsberg
Publication date: 01-06-2003
Publisher: Springer-Verlag
Published in: International Urogynecology Journal / Issue 2/2003
Print ISSN: 0937-3462
Electronic ISSN: 1433-3023
DOI: https://doi.org/10.1007/s00192-002-1020-5

At a glance: The STEP trials

Springer Medicine

Distribution of estrogen receptors α and β mRNA in mouse urogenital tissues and their expression after oophorectomy and estrogen replacement

Abstract

At a glance: The STEP trials

Springer Medicine

Abstract

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