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01-03-2014 | Original Paper

Distribution of BrdU label-retaining cells in eccrine sweat glands and comparison of the percentage of BrdU-positive cells in eccrine sweat glands and in epidermis in rats

Authors: Lu Chen, Mingjun Zhang, Haihong Li, Shijie Tang, Xiaobing Fu

Published in: Archives of Dermatological Research | Issue 2/2014

Abstract

Bromodeoxyuridine (BrdU) has commonly been used for detecting of label-retaining cells (LRCs). To determine if there are LRCs and the distributions of LRCs in eccrine sweat glands, 20 newborn SD rats within 24 h after birth were injected intraperitoneally with 50 mg/kg/time BrdU four consecutive times at 2-h intervals, or twice daily at 2-h intervals for four consecutive days. Six weeks after the last BrdU injection, rats were sacrificed, and the hind footpads were harvested, fixed and embedded in paraffin. Five-micrometer thickness tissue sections were cut and the expression of BrdU was detected immunohistochemically. The results showed that BrdU⁺ cells were scatteredly distributed in coiled secretory part and coiled duct, as well as the straight duct, but not the intraepidermal duct of eccrine sweat glands. In secretory part, besides secretory cells, myoepithelial cells showed label retaining. The percentage of BrdU⁺ cells in eccrine sweat gland of rat footpads had no significant difference between the two injection methods of BrdU (50 mg/kg/time BrdU four consecutive times at 2-h intervals vs. 50 mg/kg/time BrdU twice daily at 2-h intervals for four consecutive days) (P > 0.05). The percentage of BrdU⁺ cells in eccrine sweat glands (4.2 ± 1.3 %) was significantly higher than that in stratum basale of epidermis (0.5 ± 0.1 ‰) (P < 0.05). In conclusion, there were LRCs in eccrine sweat glands of rat footpads, and these LRCs might play important roles in the homeostasis of skin and its appendages.

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Title: Distribution of BrdU label-retaining cells in eccrine sweat glands and comparison of the percentage of BrdU-positive cells in eccrine sweat glands and in epidermis in rats
Authors: Lu Chen
Mingjun Zhang
Haihong Li
Shijie Tang
Xiaobing Fu
Publication date: 01-03-2014
Publisher: Springer Berlin Heidelberg
Published in: Archives of Dermatological Research / Issue 2/2014
Print ISSN: 0340-3696
Electronic ISSN: 1432-069X
DOI: https://doi.org/10.1007/s00403-013-1397-7

At a glance: The STEP trials

Springer Medicine

Distribution of BrdU label-retaining cells in eccrine sweat glands and comparison of the percentage of BrdU-positive cells in eccrine sweat glands and in epidermis in rats

Abstract

At a glance: The STEP trials

Springer Medicine

Abstract

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