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01-03-2009 | Original Article

Dissociation between force and maximal Na⁺, K⁺-ATPase activity in rat fast-twitch skeletal muscle with fatiguing in vitro stimulation

Authors: Craig A. Goodman, Alan Hayes, Michael J. McKenna

Published in: European Journal of Applied Physiology | Issue 4/2009

Abstract

This study investigated whether high frequency in vitro stimulation of rat fast-twitch extensor digitorum longus muscle depresses Na⁺, K⁺-ATPase (NKA) activity as measured by the maximal in vitro 3-O-MFPase assay. EDL muscles subjected to 10 s continuous 100 Hz stimulation reduced tetanic force by 51.8 ± 5.1% which recovered to 81.2 ± 6.1% after 1 min and remained stable over 1 h recovery period. A second bout reduced force by 50.3 ± 3.8% of initial but had no effect on 3-O-MFPase activity. Three minutes of intermittent stimulation (1 s at 100 Hz and 4 s recovery) resulted in 87.0 ± 2.8% decline force with slow recovery (62.7 ± 5.8% of initial after 1 h). The second 3-min bout reduced force by 83.3 ± 3.6% of initial with no change in maximal 3-O-MFPase activity. These findings contrast previous human studies where fatiguing voluntary exercise depresses maximal NKA activity. This suggests that NKA in rat fast-twitch muscle is resistant to fatigue-induced inactivation under these conditions. Furthermore, the loss of force with fatigue was not related to depressed maximal NKA activity.

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Title: Dissociation between force and maximal Na+, K+-ATPase activity in rat fast-twitch skeletal muscle with fatiguing in vitro stimulation
Authors: Craig A. Goodman
Alan Hayes
Michael J. McKenna
Publication date: 01-03-2009
Publisher: Springer-Verlag
Published in: European Journal of Applied Physiology / Issue 4/2009
Print ISSN: 1439-6319
Electronic ISSN: 1439-6327
DOI: https://doi.org/10.1007/s00421-008-0937-x

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Dissociation between force and maximal Na⁺, K⁺-ATPase activity in rat fast-twitch skeletal muscle with fatiguing in vitro stimulation

Abstract

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