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01-07-2013 | Original Paper

Direct subcutaneous injection of polyethylene particles over the murine calvaria results in dramatic osteolysis

Authors: Allison J. Rao, Stefan Zwingenberger, Roberto Valladares, Chenguang Li, Robert Lane Smith, Stuart B. Goodman, Christophe Nich

Published in: International Orthopaedics | Issue 7/2013

Abstract

Purpose

The murine calvarial model has been widely employed for the in vivo study of particle-induced osteolysis, the most frequent cause of aseptic loosening of total joint replacements. Classically, this model uses an open surgical technique in which polyethylene (PE) particles are directly spread over the calvarium for the induction of osteolysis. We evaluated a minimally invasive modification of the calvarial model by using a direct subcutaneous injection of PE particles.

Methods

Polyethylene (PE) particles were injected subcutaneously over the calvaria of C57BL6J ten-week-old mice (“injection” group) or were implanted after surgical exposure of the calvaria (“open” group) (n = 5/group). For each group, five additional mice received no particles and served as controls. Particle-induced osteolysis was evaluated two weeks after the procedure using high-definition microCT imaging.

Results

Polyethylene particle injection over the calvaria resulted in a 40 % ± 1.8 % decrease in the bone volume fraction (BVF), compared to controls. Using the “open surgical technique”, the BVF decreased by 16 % ± 3.8 % as compared to controls (p < 0.0001).

Conclusions

Direct subcutaneous injection of PE particles over the murine calvaria produced more profound resorption of bone. Polyethylene particle implantation by injection is less invasive and reliably induces osteolysis to a greater degree than the open technique. This subcutaneous injection method will prove useful for repetitive injections of particles, and the assessment of potential local or systemic therapies.

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Title: Direct subcutaneous injection of polyethylene particles over the murine calvaria results in dramatic osteolysis
Authors: Allison J. Rao
Stefan Zwingenberger
Roberto Valladares
Chenguang Li
Robert Lane Smith
Stuart B. Goodman
Christophe Nich
Publication date: 01-07-2013
Publisher: Springer-Verlag
Published in: International Orthopaedics / Issue 7/2013
Print ISSN: 0341-2695
Electronic ISSN: 1432-5195
DOI: https://doi.org/10.1007/s00264-013-1887-4

Keynote webinar | Spotlight on medication adherence

Springer Medicine

Direct subcutaneous injection of polyethylene particles over the murine calvaria results in dramatic osteolysis

Abstract

Purpose

Methods

Results

Conclusions

Keynote webinar | Spotlight on medication adherence

Springer Medicine

Abstract

Purpose

Methods

Results

Conclusions

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