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Journal of Orthopaedic Surgery and Research
Published in: Journal of Orthopaedic Surgery and Research 1/2006

Open Access 01-12-2006 | Research article

Direct effects of caffeine on osteoblastic cells metabolism: the possible causal effect of caffeine on the formation of osteoporosis

Authors: Yang-Hwei Tsuang, Jui-Sheng Sun, Li-Ting Chen, Samuel Chung-Kai Sun, San-Chi Chen

Published in: Journal of Orthopaedic Surgery and Research | Issue 1/2006

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Abstract

Background

Caffeine consumption has been reported to decrease bone mineral density (BMD), increase the risk of hip fracture, and negatively influence calcium retention. In this study, we investigated the influence of caffeine on the osteoblasts behaviour.

Method

Osteoblasts derived from newborn Wistar-rat calvaria was used in this study. The effects of various concentrations of caffeine on bone cell activities were evaluated by using MTT assay. Alkaline phosphatase (ALP) staining, von Kossa staining and biochemical parameters including ALP, lactate dehydrogenase (LDH), prostaglandin E2 (PGE2) and total protein were performed at day 1, 3, and 7. DNA degradation analysis under the caffeine influence was also performed.

Results and discussion

The results showed that the viability of the osteoblasts, the formation of ALP positive staining colonies and mineralization nodules formation in the osteoblasts cultures decreased significantly in the presence of 10 mM caffeine. The intracellular LDH, ALP and PGE2 content decreased significantly, the LDH and PGE2 secreted into the medium increased significantly. The activation of an irreversible commitment to cell death by caffeine was clearly demonstrated by DNA ladder staining.

Conclusion

In summary, our results suggest that caffeine has potential deleterious effect on the osteoblasts viability, which may enhance the rate of osteoblasts apoptosis.
Appendix
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Literature
1.
Heaney RP, Recker RR: Effects of nitrogen, phosphorus, and caffeine on calcium balance in women. J Lab & Clin Med. 1982, 99: 46-55.
2.
Massey LK, Wise KJ: The effect of dietary caffeine on urinary excretion of calcium, magnesium, sodium and potassium in healthy young females. Nutr Res. 1984, 4: 43-50. 10.1016/S0271-5317(84)80132-3.CrossRef
3.
Heaney RP: Effects of caffeine on bone and the calcium economy. Food Chem Toxicol. 2002, 40: 1263-1270. 10.1016/S0278-6915(02)00094-7.CrossRefPubMed
4.
Barrett-Connor E, Chang JC, Edelstein SL: Coffee-associated osteoporosis offset by daily milk consumption. The Rancho Bernardo Study. JAMA. 1994, 271: 280-283. 10.1001/jama.271.4.280.CrossRefPubMed
5.
Hernandez-Avila M, Colditz GA, Stampfer MJ, Rosner B, Speizer FE, Willett WC: Caffeine, moderate alcohol intake, and risk of fractures of the hip and forearm in middle-aged women. Am J Clin Nutr. 1991, 54: 157-163.PubMed
6.
Cummings SR, Nevitt MC, Browner WS, Stone K, Fox KM, Ensrud KE, Cauley J, Black D, Vogt TM: Risk factors for hip fracture in white women. Study of Osteoporotic Fractures Research Group. N Engl J Med. 1995, 332: 767-773. 10.1056/NEJM199503233321202.CrossRefPubMed
7.
Meyer HE, Pedersen JI, Loken EB, Tverdal A: Dietary factors and the incidence of hip fracture in middle-aged Norwegians – a prospective study. Am J Epidemiol. 1997, 145: 117-123.CrossRefPubMed
8.
Smith S, Swain J, Brown EM, Wyshak G, Albright T, Ravnikar VA, Schiff I: A preliminary report of the short-term effect of carbonated beverage consumption on calcium metabolism in normal women. Arch Intern Med. 1989, 149: 2517-2519. 10.1001/archinte.149.11.2517.CrossRefPubMed
9.
Hansen MA, Overgaard K, Riis BJ, Christiansen C: Potential risk factors for development of postmenopausal osteoporosis – examined over a 12-year period. Osteoporos Int. 1991, 1: 95-102. 10.1007/BF01880450.CrossRefPubMed
10.
Cooper C, Atkinson EJ, Wahner HW, O'Fallon WM, Riggs BL, Judd HL, Melton LJ: Is caffeine consumption a risk factor for osteoporosis?. J Bone Miner Res. 1992, 7: 465-471.CrossRefPubMed
11.
Reid IR, Ames RW, Evans MC, Sharpe SJ, Gamble GD: Determinants of the rate of bone loss in normal postmenopausal women. J Clin Endocrinol Metab. 1994, 79: 950-954. 10.1210/jc.79.4.950.PubMed
12.
Packard PT, Recker RR: Caffeine does not affect the rate of gain in spine bone in young women. Osteoporos Int. 1996, 6: 149-152. 10.1007/BF01623939.CrossRefPubMed
13.
Lloyd T, Rollings N, Eggli DF, Kieselhorst K, Chinchilli VM: Dietary caffeine intake and bone status of postmenopausal women. Am J Clin Nutr. 1997, 65: 1826-1830.PubMed
14.
Hannan MT, Felson DT, Dawson-Hughes B, Tucker KL, Cupples LA, Wilson PW, Kiel DP: Risk factors for longitudinal bone loss in elderly men and women: the Framingham Osteoporotic Study. J Bone Miner Res. 2000, 15: 710-720. 10.1359/jbmr.2000.15.4.710.CrossRefPubMed
15.
Rapuri PB, Gallagher JC, Kinyamu HK, Ryschon KL: Caffeine intake increases the rate of bone loss in elderly women and interacts with vitamin D receptor genotypes. Am J Clin Nutr. 2001, 74: 694-700.PubMed
16.
Sun JS, Lin CY, Dong GC, Sheu SY, Lin FH, Chen LT, Wang YJ: The Effect of Gu-Sui-Bu (Drynaria fortunei J. Sm) on Bone Cells Activities. Biomaterials. 2002, 23: 3377-3385. 10.1016/S0142-9612(02)00038-8.CrossRefPubMed
17.
Caplan AI, Pechak DG: The cellular and molecular embryology of bone formation. Bone and mineral research, Vol. 5. Elsevier, Hall AK and Dickson IR. The effects of parathyroid hormone on osteoblast-like cell from embryonic chick calvaria. Acta Endocrinol. Edited by: Peck C. 1985, 108: 217-223.
18.
Doherty MJ, Schlag G, Schwarz N, Mollan RA, Nolan PC, Wilson DJ: Biocompatibility of xenogeneic bone, commercially available coral, a bioceramic and tissue sealant for human osteoblasts. Biomaterials. 1994, 15: 601-608. 10.1016/0142-9612(94)90210-0.CrossRefPubMed
19.
Mosmann T: Rapid colorimetric assay for cellular growth and survival: application to proliferation and cytotoxicity assay. J Immunol Methods. 1983, 65: 55-63. 10.1016/0022-1759(83)90303-4.CrossRefPubMed
20.
Gundle R, Beresford JN: The isolation and culture of cells from explants of human trabecular bone. Calcif Tissue Int. 1995, 56: S8-S10.CrossRef
21.
Dews PB: Caffeine. Ann Rev Nutr. 1982, 2: 323-341. 10.1146/annurev.nu.02.070182.001543.CrossRef
22.
Sugiyama K, He P, Wada S, Saeki S: Teas and other beverages suppress D-galactosamine-induced liver injury in rats. J Nutr. 1999, 129: 1361-1367.PubMed
23.
Kiel DP, Felson DT, Hannan MT, Anderson JJ, Wilson PW: Caffeine and the risk of hip fracture: the Framingham Study. Am J Epidemiol. 1990, 132: 675-684.PubMed
24.
Kamagata-Kiyoura Y, Ohta M, Cheuk G, Yazdani M, Saltzman MJ, Nakamoto T: Combined effects of caffeine and prostaglandin E2 on the proliferation of osteoblast-like cells (UMR106-01). J Periodont. 1999, 70: 283-288. 10.1902/jop.1999.70.3.283.CrossRefPubMed
25.
Johnell O, Gullberg B, Kanis JA, Allander E, Elffors L, Dequeker J, Dilsen G, Gennari C, Lopes Vaz A, Lyritis G: Risk factors for hip fracture in European women: The MEDOS study. J Bone Miner Res. 1995, 10: 1802-1815.CrossRefPubMed
26.
Lloyd T, Rollings N, Eggli DF, Kieselhorst K, Chinchilli VM: Dietary caffeine intake and bone status of postmenopausal women. Am J Clin Nutr. 1997, 65: 1826-1830.PubMed
27.
Stein GS, Lian JB, Stein JL, Van Wijnen AJ, Montecino M: Transcriptional control of osteoblast growth and differentiation. Physiol Rev. 1996, 76: 593-629.PubMed
28.
Coelho MJ, Fernandes MH: Human bone cell cultures in biocompatibility testing. Part II: effect of ascorbic acid, beta-glycerophosphate and dexamethasone on osteoblastic differentiation. Biomaterials. 2000, 21: 1095-1102. 10.1016/S0142-9612(99)00192-1.CrossRefPubMed
29.
Harris SA, Enger RJ, Riggs BL, Spelsberg TC: Development and characterization of a conditionally immortalized human fetal osteoblastic cell line. J Bone Miner Res. 1995, 10: 178-186.CrossRefPubMed
30.
Quinn JMW, Sabokbar A, Denne M, de Vernejoul MC, McGee JO, Athanasou NA: Inhibitory and stimulatory effects of prostaglandins on osteoclast differentiation. Calcif Tissue Int. 1997, 60: 63-70. 10.1007/s002239900187.CrossRefPubMed
31.
Lerner UH, Ransjo M, Ljunggren O: Prostaglandin E2 causes a transient inhibition of mineral mobilization, matrix degradation, and lysosomal enzyme release from mouse calvarial bones in vitro. Calcif Tissue Internat. 1987, 40: 323-331.CrossRef
32.
Desimone DP, Greene VS, Hannon KS, Turner RT, Bell NH: Prostaglandin E2 administered by subcutaneous pellets causes local inflammation and systemic bone loss: a model for inflammation-induced bone disease. J Bone Miner Res. 1993, 8: 625-634.CrossRefPubMed
33.
Fuller K, Chambers TJ: Effect of arachidonic acid metabolites on bone resorption by isolated rat osteoclasts. J Bone Miner Res. 1989, 4: 209-215.CrossRefPubMed
34.
Okuda A, Taylor LM, Heersche JN: Prostaglandin E2 initially inhibits and then stimulates bone resorption in isolated rabbit osteoclast cultures. Bone Miner. 1989, 7: 255-266. 10.1016/0169-6009(89)90082-2.CrossRefPubMed
35.
Saraste A, Pulkki K: Morphologic and biochemical hallmarks of apoptosis. Cardiovasc Res. 2000, 45: 528-537. 10.1016/S0008-6363(99)00384-3.CrossRefPubMed
36.
Hoeberichts FA, Woltering EJ: Multiple mediators of plant programmed cell death: interplay of conserved cell death mechanisms and plant-specific regulators. BioEssays. 2003, 25: 47-57. 10.1002/bies.10175.CrossRefPubMed
Metadata
Title
Direct effects of caffeine on osteoblastic cells metabolism: the possible causal effect of caffeine on the formation of osteoporosis
Authors
Yang-Hwei Tsuang
Jui-Sheng Sun
Li-Ting Chen
Samuel Chung-Kai Sun
San-Chi Chen
Publication date
01-12-2006
Publisher
BioMed Central
Published in
Journal of Orthopaedic Surgery and Research / Issue 1/2006
Electronic ISSN: 1749-799X
DOI
https://doi.org/10.1186/1749-799X-1-7

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