Skip to main content
Top
Published in: BMC Oral Health 1/2022

Open Access 01-12-2022 | Diode Laser | Research

Pulpal and periapical tissue response after direct pulp capping with endosequence root repair material and low-level laser application

Authors: Loai Alsofi, Wafaa Khalil, Nada O. Binmadi, Mey A. Al-Habib, Hanan Alharbi

Published in: BMC Oral Health | Issue 1/2022

Login to get access

Abstract

Background

The study aims to investigate the pulp and periapical reaction and healing after capping with EndoSequence Root Repair Material (ERRM) combined with low-level laser application.

Methods

In 6 rabbits, pulps were exposed via class V, half of the samples received a low-level diode laser at 980 nm. Thereafter, cavities were capped with regular-set ERRM. The specimens were processed for histomorphological examination after 2 weeks and two months.

Results

After 2 weeks, images show mild inflammation and organized odontoblasts in lased group. The non-lased group shows more severe inflammation. The predentin thickness was thicker in the lased group with statistical significance (p < 0.05). After 2 months, inflammatory cells were sparse in both lased and non-lased groups. In the periapical area, group one showed dilated blood vessels and thick fibrous connective tissues. In group two, there were more numerous maturations of PDL fibers with scattered inflammatory cells and congested blood vessel.

Conclusions

Using low-level laser therapy in combination with ERRM for pulp capping shortens the inflammatory phase and enhances healing.
Literature
1.
go back to reference Mahgoub N, Alqadasi B, Aldhorae K, Assiry A, Altawili ZM, Tao H. Comparison between iRoot BP Plus (EndoSequence Root Repair Material) and mineral trioxide aggregate as pulp-capping agents: a systematic review. J Int Soc Prev Community Dent. 2019;9(6):542–52.PubMedPubMedCentral Mahgoub N, Alqadasi B, Aldhorae K, Assiry A, Altawili ZM, Tao H. Comparison between iRoot BP Plus (EndoSequence Root Repair Material) and mineral trioxide aggregate as pulp-capping agents: a systematic review. J Int Soc Prev Community Dent. 2019;9(6):542–52.PubMedPubMedCentral
2.
go back to reference Komabayashi T, Zhu Q, Eberhart R, Imai Y. Current status of direct pulp-capping materials for permanent teeth. Dent Mater. 2016;35(1):1–12.CrossRef Komabayashi T, Zhu Q, Eberhart R, Imai Y. Current status of direct pulp-capping materials for permanent teeth. Dent Mater. 2016;35(1):1–12.CrossRef
3.
go back to reference Song W, Sun W, Chen L, Yuan Z. In vivo biocompatibility and bioactivity of calcium silicate-based bioceramics in endodontics. Front Bioeng Biotechnol. 2020;8:1113.CrossRef Song W, Sun W, Chen L, Yuan Z. In vivo biocompatibility and bioactivity of calcium silicate-based bioceramics in endodontics. Front Bioeng Biotechnol. 2020;8:1113.CrossRef
4.
go back to reference Giraud T, Jeanneau C, Rombouts C, Bakhtiar H, Laurent P, About I. Pulp capping materials modulate the balance between inflammation and regeneration. Dent Mater. 2019;35(1):24–35.PubMedCrossRef Giraud T, Jeanneau C, Rombouts C, Bakhtiar H, Laurent P, About I. Pulp capping materials modulate the balance between inflammation and regeneration. Dent Mater. 2019;35(1):24–35.PubMedCrossRef
5.
go back to reference Komabayashi T, Zhu Q, Eberhart R, Imai Y. Current status of direct pulp-capping materials for permanent teeth. J Endod. 2016;35(1):1–12. Komabayashi T, Zhu Q, Eberhart R, Imai Y. Current status of direct pulp-capping materials for permanent teeth. J Endod. 2016;35(1):1–12.
6.
go back to reference Dammaschke T, Stratmann U, Wolff P, Sagheri D, Schäfer E. Direct pulp capping with mineral trioxide aggregate: an immunohistologic comparison with calcium hydroxide in rodents. J Endod. 2010;36(5):814–9.PubMedCrossRef Dammaschke T, Stratmann U, Wolff P, Sagheri D, Schäfer E. Direct pulp capping with mineral trioxide aggregate: an immunohistologic comparison with calcium hydroxide in rodents. J Endod. 2010;36(5):814–9.PubMedCrossRef
7.
go back to reference AlShwaimi E, Majeed A, Ali AA. Pulpal responses to direct capping with betamethasone/gentamicin cream and mineral trioxide aggregate: histologic and micro–computed tomography assessments. J Endod. 2016;42(1):30–5.PubMedCrossRef AlShwaimi E, Majeed A, Ali AA. Pulpal responses to direct capping with betamethasone/gentamicin cream and mineral trioxide aggregate: histologic and micro–computed tomography assessments. J Endod. 2016;42(1):30–5.PubMedCrossRef
8.
go back to reference Jalan AL, Warhadpande MM, Dakshindas DM. A comparison of human dental pulp response to calcium hydroxide and Biodentine as direct pulp-capping agents. J Conserv Dent. 2017;20(2):129–33.PubMedPubMedCentralCrossRef Jalan AL, Warhadpande MM, Dakshindas DM. A comparison of human dental pulp response to calcium hydroxide and Biodentine as direct pulp-capping agents. J Conserv Dent. 2017;20(2):129–33.PubMedPubMedCentralCrossRef
9.
go back to reference Heller AL, Koenigs JF, Brilliant JD, Melfi RC, Driskell TD. Direct pulp capping of permanent teeth in primates using a resorbable form of tricalcium phosphate ceramic. J Endod. 1975;1(3):95–101.PubMedCrossRef Heller AL, Koenigs JF, Brilliant JD, Melfi RC, Driskell TD. Direct pulp capping of permanent teeth in primates using a resorbable form of tricalcium phosphate ceramic. J Endod. 1975;1(3):95–101.PubMedCrossRef
10.
go back to reference Peskersoy C, Lukarcanin J, Turkun M. Efficacy of different calcium silicate materials as pulp-capping agents: randomized clinical trial. J Dent Sci. 2020;16:723–31.PubMedPubMedCentralCrossRef Peskersoy C, Lukarcanin J, Turkun M. Efficacy of different calcium silicate materials as pulp-capping agents: randomized clinical trial. J Dent Sci. 2020;16:723–31.PubMedPubMedCentralCrossRef
11.
go back to reference Tran XV, Gorin C, Willig C, Baroukh B, Pellat B, Decup F, Opsahl Vital S, Chaussain C, Boukpessi T. Effect of a calcium-silicate-based restorative cement on pulp repair. J Dent Res. 2012;91:1166–71.PubMedCrossRef Tran XV, Gorin C, Willig C, Baroukh B, Pellat B, Decup F, Opsahl Vital S, Chaussain C, Boukpessi T. Effect of a calcium-silicate-based restorative cement on pulp repair. J Dent Res. 2012;91:1166–71.PubMedCrossRef
12.
go back to reference De-Deus G, Cardoso ML, Belladonna FG, Cavalcante DM, Simões-Carvalho M, Souza EM, Lopes RT, Silva EJ. Performance of Reciproc Blue R25 instruments in shaping the canal space without glide path. J Endod. 2019;45:194–8.PubMedCrossRef De-Deus G, Cardoso ML, Belladonna FG, Cavalcante DM, Simões-Carvalho M, Souza EM, Lopes RT, Silva EJ. Performance of Reciproc Blue R25 instruments in shaping the canal space without glide path. J Endod. 2019;45:194–8.PubMedCrossRef
13.
go back to reference Komabayashi T, Ebihara A, Aoki A. The use of lasers for direct pulp capping. J Oral Sci. 2015;57(4):277–86.PubMedCrossRef Komabayashi T, Ebihara A, Aoki A. The use of lasers for direct pulp capping. J Oral Sci. 2015;57(4):277–86.PubMedCrossRef
14.
go back to reference Utsunomiya T. A histopathological study of the effects of low-power laser irradiation on wound healing of exposed dental pulp tissues in dogs, with special reference to lectins and collagens. J Endod. 1998;24(3):187–93.PubMedCrossRef Utsunomiya T. A histopathological study of the effects of low-power laser irradiation on wound healing of exposed dental pulp tissues in dogs, with special reference to lectins and collagens. J Endod. 1998;24(3):187–93.PubMedCrossRef
15.
go back to reference Javed FA-O, Kellesarian SV, Abduljabbar T, Gholamiazizi E, Feng C, Aldosary K, Vohra F, Romanos GE. Role of laser irradiation in direct pulp capping procedures: a systematic review and meta-analysis. Laser Med Sci. 2017;32(2):439–48.CrossRef Javed FA-O, Kellesarian SV, Abduljabbar T, Gholamiazizi E, Feng C, Aldosary K, Vohra F, Romanos GE. Role of laser irradiation in direct pulp capping procedures: a systematic review and meta-analysis. Laser Med Sci. 2017;32(2):439–48.CrossRef
16.
go back to reference Yazdanfar I, Gutknecht N, Franzen R. Effects of diode laser on direct pulp capping treatment: a pilot study. Lasers Med Sci. 2015;30(4):1237–43.PubMedCrossRef Yazdanfar I, Gutknecht N, Franzen R. Effects of diode laser on direct pulp capping treatment: a pilot study. Lasers Med Sci. 2015;30(4):1237–43.PubMedCrossRef
17.
go back to reference Bidar M, Moushekhian S, Gharechahi M, Talati A, Ahrari F, Bojarpour M. The effect of low-level laser therapy on direct pulp capping in dogs. J Lasers Med Sci. 2016;7(3):177–83.PubMedPubMedCentralCrossRef Bidar M, Moushekhian S, Gharechahi M, Talati A, Ahrari F, Bojarpour M. The effect of low-level laser therapy on direct pulp capping in dogs. J Lasers Med Sci. 2016;7(3):177–83.PubMedPubMedCentralCrossRef
18.
go back to reference Yamakawa S, Niwa T, Karakida T, Kobayashi K, Yamamoto R, Chiba R, Yamakoshi Y, Hosoya N. Effects of Er: YAG and diode laser irradiation on dental pulp cells and tissues. Int J Mol Sci. 2018;19(8):2429.PubMedCentralCrossRef Yamakawa S, Niwa T, Karakida T, Kobayashi K, Yamamoto R, Chiba R, Yamakoshi Y, Hosoya N. Effects of Er: YAG and diode laser irradiation on dental pulp cells and tissues. Int J Mol Sci. 2018;19(8):2429.PubMedCentralCrossRef
19.
go back to reference Godoy BM, Arana-Chavez VE, Nunez SC, Ribeiro MS. Effects of low-power red laser on dentine-pulp interface after cavity preparation. An ultrastructural study. Arch Oral Biol. 2007;52(9):899–903.PubMedCrossRef Godoy BM, Arana-Chavez VE, Nunez SC, Ribeiro MS. Effects of low-power red laser on dentine-pulp interface after cavity preparation. An ultrastructural study. Arch Oral Biol. 2007;52(9):899–903.PubMedCrossRef
20.
go back to reference Yasuda Y, Ohtomo E, Tsukuba T, Okamoto K, Saito T. Carbon dioxide laser irradiation stimulates mineralization in rat dental pulp cells. Int Endod J. 2009;42(10):940–6.PubMedCrossRef Yasuda Y, Ohtomo E, Tsukuba T, Okamoto K, Saito T. Carbon dioxide laser irradiation stimulates mineralization in rat dental pulp cells. Int Endod J. 2009;42(10):940–6.PubMedCrossRef
21.
go back to reference Carroll JD, Milward MR, Cooper PR, Hadis M, Palin WM. Developments in low-level light therapy (LLLT) for dentistry. Dent Mater. 2014;30(5):465–75.PubMedCrossRef Carroll JD, Milward MR, Cooper PR, Hadis M, Palin WM. Developments in low-level light therapy (LLLT) for dentistry. Dent Mater. 2014;30(5):465–75.PubMedCrossRef
22.
go back to reference Choung HW, Lee SH, Ham AR, Lee NR, Kim B, Pang KM, Jahng JW, Lee JH. Effectiveness of low-level laser therapy with a 915 Nm wavelength diode laser on the healing of intraoral mucosal wound: an animal study and a double-blind randomized clinical trial. Medicina (Kaunas). 2019;55(8):405.CrossRef Choung HW, Lee SH, Ham AR, Lee NR, Kim B, Pang KM, Jahng JW, Lee JH. Effectiveness of low-level laser therapy with a 915 Nm wavelength diode laser on the healing of intraoral mucosal wound: an animal study and a double-blind randomized clinical trial. Medicina (Kaunas). 2019;55(8):405.CrossRef
23.
go back to reference Sakata S, Kunimatsu R, Tsuka Y, Nakatani A, Hiraki T, Gunji H, Hirose N, Yanoshita M, Putranti NAR, Tanimoto K. High-frequency near-infrared diode laser irradiation attenuates il-1β-induced expression of inflammatory cytokines and matrix metalloproteinases in human primary chondrocytes. J Clin Med. 2020;9(3):881.PubMedCentralCrossRef Sakata S, Kunimatsu R, Tsuka Y, Nakatani A, Hiraki T, Gunji H, Hirose N, Yanoshita M, Putranti NAR, Tanimoto K. High-frequency near-infrared diode laser irradiation attenuates il-1β-induced expression of inflammatory cytokines and matrix metalloproteinases in human primary chondrocytes. J Clin Med. 2020;9(3):881.PubMedCentralCrossRef
24.
go back to reference Alves ACA, de Paula Vieira R, Leal-Junior EC, dos Santos SA, Ligeiro AP, Albertini R, Junior JA, de Carvalho PD. Effect of low-level laser therapy on the expression of inflammatory mediators and on neutrophils and macrophages in acute joint inflammation. Arthr Res Ther. 2013;15(5):1–1.CrossRef Alves ACA, de Paula Vieira R, Leal-Junior EC, dos Santos SA, Ligeiro AP, Albertini R, Junior JA, de Carvalho PD. Effect of low-level laser therapy on the expression of inflammatory mediators and on neutrophils and macrophages in acute joint inflammation. Arthr Res Ther. 2013;15(5):1–1.CrossRef
25.
go back to reference El Nawam H, El Backly R, Zaky A, Abdallah A. Low-level laser therapy affects dentinogenesis and angiogenesis of in vitro 3D cultures of dentin-pulp complex. Lasers Med Sci. 2019;34(8):1689–98.PubMedCrossRef El Nawam H, El Backly R, Zaky A, Abdallah A. Low-level laser therapy affects dentinogenesis and angiogenesis of in vitro 3D cultures of dentin-pulp complex. Lasers Med Sci. 2019;34(8):1689–98.PubMedCrossRef
26.
go back to reference Arany PR, Cho A, Hunt TD, Sidhu G, Shin K, Hahm E, Huang GX, Weaver J, Chen AC, Padwa BL, et al. Photoactivation of endogenous latent transforming growth factor-β1 directs dental stem cell differentiation for regeneration. Sci Transl Med. 2014;6(238):238–69.CrossRef Arany PR, Cho A, Hunt TD, Sidhu G, Shin K, Hahm E, Huang GX, Weaver J, Chen AC, Padwa BL, et al. Photoactivation of endogenous latent transforming growth factor-β1 directs dental stem cell differentiation for regeneration. Sci Transl Med. 2014;6(238):238–69.CrossRef
27.
go back to reference Verstraete FJ, Osofsky A. Dentistry in pet rabbits. Compend Contin Educ Pract Vet. 2005;27(9):671–84. Verstraete FJ, Osofsky A. Dentistry in pet rabbits. Compend Contin Educ Pract Vet. 2005;27(9):671–84.
28.
go back to reference Amin LE, Montaser M. Comparative evaluation of pulpal repair after direct pulp capping using stem cell therapy and biodentine: an animal study. Aust Endod J. 2021;47(1):11–9.PubMedCrossRef Amin LE, Montaser M. Comparative evaluation of pulpal repair after direct pulp capping using stem cell therapy and biodentine: an animal study. Aust Endod J. 2021;47(1):11–9.PubMedCrossRef
29.
go back to reference Khalil WA, Abunasef SK. Can mineral trioxide aggregate and nanoparticulate endosequence root repair material produce injurious effects to rat subcutaneous tissues? J Endod. 2015;41(7):1151–6.PubMedCrossRef Khalil WA, Abunasef SK. Can mineral trioxide aggregate and nanoparticulate endosequence root repair material produce injurious effects to rat subcutaneous tissues? J Endod. 2015;41(7):1151–6.PubMedCrossRef
30.
go back to reference Koliniotou-Koumpia E, Tziafas D. Pulpal responses following direct pulp capping of healthy dog teeth with dentine adhesive systems. J Dent. 2005;33(8):639–47.PubMedCrossRef Koliniotou-Koumpia E, Tziafas D. Pulpal responses following direct pulp capping of healthy dog teeth with dentine adhesive systems. J Dent. 2005;33(8):639–47.PubMedCrossRef
31.
go back to reference Misuriya A, Bhardwaj A, Bhardwaj A, Aggrawal S, Kumar PP, Gajjarepu S. A comparative antimicrobial analysis of various root canal irrigating solutions on endodontic pathogens: an in vitro study. J Contemp Dent Pract. 2014;15(2):153–60.PubMedCrossRef Misuriya A, Bhardwaj A, Bhardwaj A, Aggrawal S, Kumar PP, Gajjarepu S. A comparative antimicrobial analysis of various root canal irrigating solutions on endodontic pathogens: an in vitro study. J Contemp Dent Pract. 2014;15(2):153–60.PubMedCrossRef
32.
go back to reference Parolia A, Kundabala M, Rao NN, Acharya SR, Agrawal P, Mohan M, Thomas M. A comparative histological analysis of human pulp following direct pulp capping with Propolis, mineral trioxide aggregate and Dycal. Aust Dent J. 2010;55(1):59–64.PubMedCrossRef Parolia A, Kundabala M, Rao NN, Acharya SR, Agrawal P, Mohan M, Thomas M. A comparative histological analysis of human pulp following direct pulp capping with Propolis, mineral trioxide aggregate and Dycal. Aust Dent J. 2010;55(1):59–64.PubMedCrossRef
33.
go back to reference Nowicka AA-O, Łagocka RA-O, Lipski MA-O, Parafiniuk M, Grocholewicz K, Sobolewska E, Witek AA-O, Buczkowska-Radlińska J. Clinical and histological evaluation of direct pulp capping on human pulp tissue using a dentin adhesive system. Biomed Res Int. 2016;2016:2591273.PubMedPubMedCentralCrossRef Nowicka AA-O, Łagocka RA-O, Lipski MA-O, Parafiniuk M, Grocholewicz K, Sobolewska E, Witek AA-O, Buczkowska-Radlińska J. Clinical and histological evaluation of direct pulp capping on human pulp tissue using a dentin adhesive system. Biomed Res Int. 2016;2016:2591273.PubMedPubMedCentralCrossRef
34.
go back to reference Basandi PS, Madammal RM, Adi RP, Donoghue M, Nayak S, Manickam S. Predentin thickness analysis in developing and developed permanent teeth. J Nat Sci Biol Med. 2015;6(2):310–3.PubMedPubMedCentralCrossRef Basandi PS, Madammal RM, Adi RP, Donoghue M, Nayak S, Manickam S. Predentin thickness analysis in developing and developed permanent teeth. J Nat Sci Biol Med. 2015;6(2):310–3.PubMedPubMedCentralCrossRef
35.
go back to reference Al-Agele A, Jamil A, Abdulghani B. In vivo histological evaluation of effect of direct pulp capping with BMP7 with and without laser therapy. J Pharm Sci&Res. 2019;11(6):2019–295. Al-Agele A, Jamil A, Abdulghani B. In vivo histological evaluation of effect of direct pulp capping with BMP7 with and without laser therapy. J Pharm Sci&Res. 2019;11(6):2019–295.
36.
go back to reference Olsson B, Sliwkowski A, Langeland K. Subcutaneous implantation for the biological evaluation of endodontic materials. J Endod. 1981;7(8):355–69.PubMedCrossRef Olsson B, Sliwkowski A, Langeland K. Subcutaneous implantation for the biological evaluation of endodontic materials. J Endod. 1981;7(8):355–69.PubMedCrossRef
38.
go back to reference Campillo VE, Langonnet S, Pierrefeu A, Chaux-Bodard AG. Anatomic and histological study of the rabbit mandible as an experimental model for wound healing and surgical therapies. Lab Anim. 2014;48(4):273–7.PubMedCrossRef Campillo VE, Langonnet S, Pierrefeu A, Chaux-Bodard AG. Anatomic and histological study of the rabbit mandible as an experimental model for wound healing and surgical therapies. Lab Anim. 2014;48(4):273–7.PubMedCrossRef
39.
go back to reference Javed F, Kellesarian SV, Abduljabbar T, Gholamiazizi E, Feng C, Aldosary K, Vohra F, Romanos GE. Role of laser irradiation in direct pulp capping procedures: a systematic review and meta-analysis. Lasers Med Sci. 2017;32(2):439–48.PubMedCrossRef Javed F, Kellesarian SV, Abduljabbar T, Gholamiazizi E, Feng C, Aldosary K, Vohra F, Romanos GE. Role of laser irradiation in direct pulp capping procedures: a systematic review and meta-analysis. Lasers Med Sci. 2017;32(2):439–48.PubMedCrossRef
40.
go back to reference Basandi PS, Madammal RM, Adi RP, Donoghue M, Nayak S, Manickam S. Predentin thickness analysis in developing and developed permanent teeth. J Nat Sci Biol Med. 2015;6(2):310–3.PubMedPubMedCentralCrossRef Basandi PS, Madammal RM, Adi RP, Donoghue M, Nayak S, Manickam S. Predentin thickness analysis in developing and developed permanent teeth. J Nat Sci Biol Med. 2015;6(2):310–3.PubMedPubMedCentralCrossRef
41.
go back to reference Fekrazad R, Seraj B, Ghadimi S, Tamiz P, Mottahary P, Dehghan MM. The effect of low-level laser therapy (810 nm) on root development of immature permanent teeth in dogs. Lasers Med Sci. 2015;30(4):1251–7.PubMedCrossRef Fekrazad R, Seraj B, Ghadimi S, Tamiz P, Mottahary P, Dehghan MM. The effect of low-level laser therapy (810 nm) on root development of immature permanent teeth in dogs. Lasers Med Sci. 2015;30(4):1251–7.PubMedCrossRef
42.
go back to reference Fiório FB, Albertini R, Leal-Junior ECP. de Carvalho PdTC: Effect of low-level laser therapy on types I and III collagen and inflammatory cells in rats with induced third-degree burns. Lasers Med Sci. 2014;29(1):313–9.PubMedCrossRef Fiório FB, Albertini R, Leal-Junior ECP. de Carvalho PdTC: Effect of low-level laser therapy on types I and III collagen and inflammatory cells in rats with induced third-degree burns. Lasers Med Sci. 2014;29(1):313–9.PubMedCrossRef
43.
go back to reference Yazdanfar I, Barekatain M, Zare Jahromi M. Combination effects of diode laser and resin-modified tricalcium silicate on direct pulp capping treatment of caries exposures in permanent teeth: a randomized clinical trial. Lasers Med Sci. 2020;35(8):1849–55.PubMedCrossRef Yazdanfar I, Barekatain M, Zare Jahromi M. Combination effects of diode laser and resin-modified tricalcium silicate on direct pulp capping treatment of caries exposures in permanent teeth: a randomized clinical trial. Lasers Med Sci. 2020;35(8):1849–55.PubMedCrossRef
44.
go back to reference Ferriello V, Faria MR, Cavalcanti BN. The effects of low-level diode laser treatment and dental pulp-capping materials on the proliferation of L-929 fibroblasts. J Oral Sci. 2010;52(1):33–8.PubMedCrossRef Ferriello V, Faria MR, Cavalcanti BN. The effects of low-level diode laser treatment and dental pulp-capping materials on the proliferation of L-929 fibroblasts. J Oral Sci. 2010;52(1):33–8.PubMedCrossRef
Metadata
Title
Pulpal and periapical tissue response after direct pulp capping with endosequence root repair material and low-level laser application
Authors
Loai Alsofi
Wafaa Khalil
Nada O. Binmadi
Mey A. Al-Habib
Hanan Alharbi
Publication date
01-12-2022
Publisher
BioMed Central
Keywords
Diode Laser
Laser
Published in
BMC Oral Health / Issue 1/2022
Electronic ISSN: 1472-6831
DOI
https://doi.org/10.1186/s12903-022-02099-0

Other articles of this Issue 1/2022

BMC Oral Health 1/2022 Go to the issue