Skip to main content
Top
Published in: Lasers in Dental Science 3/2019

01-09-2019 | Diode Laser | Original Article

In vitro evaluation of the push-out bond strength of the epoxy resin-based root canal sealer to root dentin irradiated with double-wavelength lasers

Authors: Massoud Rahati, Ezatolah Kazeminejad, Sareh Said Yekta-Michael, Norbert Gutknecht

Published in: Lasers in Dental Science | Issue 3/2019

Login to get access

Abstract

Purpose

The influence of dual-wavelength lasers Er,Cr:YSGG 2780 nm and diode 940 nm on bond strength of the epoxy resin-based sealer (AH Plus) to the root dentin was evaluated.

Methods

Thirty-three extracted human mandibular and maxillary straight and single-rooted teeth were sectioned at the cementoenamel junction perpendicular to the long axis. All the teeth were endodontically prepared and cut with thin disc 1.1 mm thickness transversally to the long axis. The teeth were randomly divided into three groups (n = 40). In group 1, 1 ml of NaOCl (5.25%) was used between every drill exchange for irradiating the canals. Group 2 was irradiated with EDTA (17%) and NaOCl in order to remove the smear layer from the canals. In group 3, the canals irradiated with 2780 nm Er,Cr:YSGG laser using radial firing tips and 940 nm diode laser. One root from every group was selected for scanning electron microscopy (SEM) analysis. Statistical analysis was performed using the Shapiro–Wilk test and one-way ANOVA test.

Results

Statistically significant differences were observed in group 2 (EDTA Plus NaOCl) compared with the two other groups (p < 0.001). There were no significant differences between the laser and control group (p = 0.987). The mode of failure in all groups was the cohesion failure indicating the failure within the sealer. SEM examination confirmed the presence of a thick smear layer in group 1 and open dentinal orifices in groups 2 and 3.

Conclusion

In spite of removing the smear layer, the dual-wavelength laser has no effect on the push-out bond strength of AH Plus sealer.
Literature
1.
go back to reference Sjogren U, Hagglund B, Sundqvist G, Wing K (1990) Factors affecting the long-term results of endodontic treatment. J End 16:498–504CrossRef Sjogren U, Hagglund B, Sundqvist G, Wing K (1990) Factors affecting the long-term results of endodontic treatment. J End 16:498–504CrossRef
2.
3.
go back to reference Shahravan A, Haghdoost AA, Adl A, Rahimi H, Shadifar F (2007) Effect of smear layer on sealing ability of canal obturation: a systematic review and meta-analysis. J Endod 33(2):96–105CrossRefPubMed Shahravan A, Haghdoost AA, Adl A, Rahimi H, Shadifar F (2007) Effect of smear layer on sealing ability of canal obturation: a systematic review and meta-analysis. J Endod 33(2):96–105CrossRefPubMed
4.
go back to reference Clark-Holke D, Drake D, Walton R, Rivera E, Guthmiller JM (2003) Bacterial penetration through canals of endodontically treated teeth in the presence or absence of the smear layer. J Dent 31:275–281CrossRefPubMed Clark-Holke D, Drake D, Walton R, Rivera E, Guthmiller JM (2003) Bacterial penetration through canals of endodontically treated teeth in the presence or absence of the smear layer. J Dent 31:275–281CrossRefPubMed
5.
go back to reference Cobankara FK, Adanr N, Belli S (2004) Evaluation of the influence of smear layer on the apical and coronal sealing ability of two sealers. J Endod 30:406–409CrossRefPubMed Cobankara FK, Adanr N, Belli S (2004) Evaluation of the influence of smear layer on the apical and coronal sealing ability of two sealers. J Endod 30:406–409CrossRefPubMed
6.
go back to reference Hulsmann M, Heckendorff M, Lennon A (2003) Chelating agents in root canal treatment: mode of action and indications for their use. Int Endod J 36:810–830CrossRefPubMed Hulsmann M, Heckendorff M, Lennon A (2003) Chelating agents in root canal treatment: mode of action and indications for their use. Int Endod J 36:810–830CrossRefPubMed
7.
go back to reference Crumpton BJ, Goodell GG, McClanahan SB (2005) Effects on smear layer and debris removal with varying volumes of 17% REDTA after rotary instrumentation. J Endod 31:536–538CrossRefPubMed Crumpton BJ, Goodell GG, McClanahan SB (2005) Effects on smear layer and debris removal with varying volumes of 17% REDTA after rotary instrumentation. J Endod 31:536–538CrossRefPubMed
8.
go back to reference Siqueira JF Jr, Magalhaes KM, Rocas IN (2007) Bacterial reduction in infected root canals treated with 2.5% NaOCl as an irrigant and calcium hydroxide/camphorated paramonochlorophenol paste as an intracanal dressing. J Endod 33(6):667–672CrossRefPubMed Siqueira JF Jr, Magalhaes KM, Rocas IN (2007) Bacterial reduction in infected root canals treated with 2.5% NaOCl as an irrigant and calcium hydroxide/camphorated paramonochlorophenol paste as an intracanal dressing. J Endod 33(6):667–672CrossRefPubMed
9.
go back to reference Kouchi Y, Ninomiya J, Yasuda H, Fukui K, Moriyama T, Okamoto H (1980) Location of Streptococcus mutans in the dentinal tubules of open infected root canals. J Dent Res 59:2038–2046CrossRefPubMed Kouchi Y, Ninomiya J, Yasuda H, Fukui K, Moriyama T, Okamoto H (1980) Location of Streptococcus mutans in the dentinal tubules of open infected root canals. J Dent Res 59:2038–2046CrossRefPubMed
10.
go back to reference Spratt DA, Pratten J, Wilson M, Gulabivala K (2001) An in vitro evaluation of the antimicrobial efficacy of irrigants on biofilms of root canal isolates. Int Endod J 34:300–307CrossRefPubMed Spratt DA, Pratten J, Wilson M, Gulabivala K (2001) An in vitro evaluation of the antimicrobial efficacy of irrigants on biofilms of root canal isolates. Int Endod J 34:300–307CrossRefPubMed
11.
go back to reference George R, Meyers IA, Walsh LJ (2008) Laser activation of endodontic irrigants with improved conical laser fiber tips for removing smear layer in the apical third of the root canal. J Endod 34:1524–1527CrossRefPubMed George R, Meyers IA, Walsh LJ (2008) Laser activation of endodontic irrigants with improved conical laser fiber tips for removing smear layer in the apical third of the root canal. J Endod 34:1524–1527CrossRefPubMed
12.
go back to reference Takeda FH, Harashima T, Kimura Y, Matsumoto K (1998) Comparative study about the removal of smear layer by three types of laser devices. J Clin Laser Med Surg 16(2):117–122CrossRefPubMed Takeda FH, Harashima T, Kimura Y, Matsumoto K (1998) Comparative study about the removal of smear layer by three types of laser devices. J Clin Laser Med Surg 16(2):117–122CrossRefPubMed
13.
go back to reference Gutknecht N, van Gogswaardt D, Conrads G, Apel C, Schubert C, Lampert F (2000) Diode laser radiation and its bactericidal effect in root canal wall dentin. J Clin Laser Med Surg 18(2):57–60CrossRefPubMed Gutknecht N, van Gogswaardt D, Conrads G, Apel C, Schubert C, Lampert F (2000) Diode laser radiation and its bactericidal effect in root canal wall dentin. J Clin Laser Med Surg 18(2):57–60CrossRefPubMed
14.
go back to reference Fegan SE, Steiman HR (1995) Comparative evaluation of the antibacterial effects of intracanal Nd:YAG laser irradiation: an in vitro study. J Endod 21(8):415–417CrossRefPubMed Fegan SE, Steiman HR (1995) Comparative evaluation of the antibacterial effects of intracanal Nd:YAG laser irradiation: an in vitro study. J Endod 21(8):415–417CrossRefPubMed
15.
go back to reference Silva AC, Guglielmi C, Meneguzzo DT, Aranha AC, Bombana AC, de Paula Eduardo C (2010) Analysis of permeability and morphology of root canal dentin after Er,Cr:YSGG laser irradiation. Photomed Laser Surg 28(1):103–108CrossRefPubMed Silva AC, Guglielmi C, Meneguzzo DT, Aranha AC, Bombana AC, de Paula Eduardo C (2010) Analysis of permeability and morphology of root canal dentin after Er,Cr:YSGG laser irradiation. Photomed Laser Surg 28(1):103–108CrossRefPubMed
16.
go back to reference Varella CH, Pileggi R (2007) Obturation of root canal system treated by Cr, Er: YSGG laser irradiation. J Endod 33(9):1091–1093CrossRefPubMed Varella CH, Pileggi R (2007) Obturation of root canal system treated by Cr, Er: YSGG laser irradiation. J Endod 33(9):1091–1093CrossRefPubMed
17.
go back to reference Eldeniz AU, Ozer F, Hadimli HH, Erganis O (2007) Bactericidal efficacy of Er, Cr:YSGG laser irradiation against Enterococcus faecalis compared with NaOCl irrigation: an ex vivo pilot study. Int Endod J 40(2):112–119CrossRefPubMed Eldeniz AU, Ozer F, Hadimli HH, Erganis O (2007) Bactericidal efficacy of Er, Cr:YSGG laser irradiation against Enterococcus faecalis compared with NaOCl irrigation: an ex vivo pilot study. Int Endod J 40(2):112–119CrossRefPubMed
18.
go back to reference Wang QQ, Zhang CF, Yin XZ (2007) Evaluation of the bactericidal effect of Er, Cr:YSGG, and Nd:YAG lasers in experimentally infected root canals. J Endod 33(7):830–832CrossRefPubMed Wang QQ, Zhang CF, Yin XZ (2007) Evaluation of the bactericidal effect of Er, Cr:YSGG, and Nd:YAG lasers in experimentally infected root canals. J Endod 33(7):830–832CrossRefPubMed
19.
go back to reference Ishizaki NT, Matsumoto K, Kimura Y, Wang X, Kinoshita J, Okano SM et al (2004) Thermographical and morphological studies of Er,Cr:YSGG laser irradiation on root canal walls. Photomed Laser Surg 22(4):291–297CrossRefPubMed Ishizaki NT, Matsumoto K, Kimura Y, Wang X, Kinoshita J, Okano SM et al (2004) Thermographical and morphological studies of Er,Cr:YSGG laser irradiation on root canal walls. Photomed Laser Surg 22(4):291–297CrossRefPubMed
20.
go back to reference George R, Walsh LJ (2010) Thermal effects from modified endodontic laser tips used in the apical third of root canals with erbium-doped yttrium aluminum garnet and erbium, chromium-doped yttrium scandium gallium garnet lasers. Photomed Laser Surg 28:161–165CrossRefPubMed George R, Walsh LJ (2010) Thermal effects from modified endodontic laser tips used in the apical third of root canals with erbium-doped yttrium aluminum garnet and erbium, chromium-doped yttrium scandium gallium garnet lasers. Photomed Laser Surg 28:161–165CrossRefPubMed
21.
go back to reference Martins MR, Carvalho MF, Vaz IP, Capelas JA, Martins MA, Gutknecht N (2013) Efficacy of Er,Cr:YSGG laser with endodontical radial firing tips on the outcome of endodontic treatment: blind randomized controlled clinical trial with six-month evaluation. Lasers Med Sci 28:1049–1055CrossRefPubMed Martins MR, Carvalho MF, Vaz IP, Capelas JA, Martins MA, Gutknecht N (2013) Efficacy of Er,Cr:YSGG laser with endodontical radial firing tips on the outcome of endodontic treatment: blind randomized controlled clinical trial with six-month evaluation. Lasers Med Sci 28:1049–1055CrossRefPubMed
22.
go back to reference Schoop U, Barylyak A, Goharkhay K, Beer F, Wernisch J, Georgopoulos A, Sperr W, Moritz A (2009) The impact of an erbium, chromium:yttrium-scandium-gallium-garnet laser with radial-firing tips on endodontic treatment. Lasers Med Sci 24:59–65CrossRefPubMed Schoop U, Barylyak A, Goharkhay K, Beer F, Wernisch J, Georgopoulos A, Sperr W, Moritz A (2009) The impact of an erbium, chromium:yttrium-scandium-gallium-garnet laser with radial-firing tips on endodontic treatment. Lasers Med Sci 24:59–65CrossRefPubMed
23.
go back to reference Licata ME, Albanese A, Campisi G, Geraci DM, Russo R, Gallina G (2015) Effectiveness of a new method of disinfecting the root canal using Er,Cr:YSGG laser to kill Enterococcus faecalis in an infected tooth model. Lasers Med Sci 30(2):707–712CrossRefPubMed Licata ME, Albanese A, Campisi G, Geraci DM, Russo R, Gallina G (2015) Effectiveness of a new method of disinfecting the root canal using Er,Cr:YSGG laser to kill Enterococcus faecalis in an infected tooth model. Lasers Med Sci 30(2):707–712CrossRefPubMed
24.
go back to reference Bago Juric I, Plecko V, Anic I (2014) Antimicrobial efficacy of Er,Cr:YSGG laser-activated irrigation compared with passive ultrasonic irrigation and RinsEndo((R)) against intracanal Enterococcus faecalis. Photomed Laser Surg 32(11):600–605CrossRefPubMed Bago Juric I, Plecko V, Anic I (2014) Antimicrobial efficacy of Er,Cr:YSGG laser-activated irrigation compared with passive ultrasonic irrigation and RinsEndo((R)) against intracanal Enterococcus faecalis. Photomed Laser Surg 32(11):600–605CrossRefPubMed
25.
go back to reference Falkenstein F, Gutknecht N, Franzen R (2014) Analysis of laser transmission and thermal effects on the inner root surface during periodontal treatment with a 940-nm diode laser in an in vitro pocket model. J Biomed Opt 19(12):128002CrossRefPubMed Falkenstein F, Gutknecht N, Franzen R (2014) Analysis of laser transmission and thermal effects on the inner root surface during periodontal treatment with a 940-nm diode laser in an in vitro pocket model. J Biomed Opt 19(12):128002CrossRefPubMed
26.
go back to reference Haidary D, Franzen R, Gutknecht N (2016) Root surface temperature changes during root canal laser irradiation with dual wavelength laser (940 and 2780 nm): a preliminary study. Photomed Laser Surg 34(8):336–334CrossRefPubMed Haidary D, Franzen R, Gutknecht N (2016) Root surface temperature changes during root canal laser irradiation with dual wavelength laser (940 and 2780 nm): a preliminary study. Photomed Laser Surg 34(8):336–334CrossRefPubMed
27.
go back to reference Al-Karadaghi TS, Franzen R, Jawad HA, Gutknecht N (2015) Investigations of radicular dentin permeability and ultrastructural changes after irradiation with Er,Cr:YSGG laser and dual wavelength (2780 and 940 nm) laser. Lasers Med Sci 30(8):2115–2121CrossRefPubMed Al-Karadaghi TS, Franzen R, Jawad HA, Gutknecht N (2015) Investigations of radicular dentin permeability and ultrastructural changes after irradiation with Er,Cr:YSGG laser and dual wavelength (2780 and 940 nm) laser. Lasers Med Sci 30(8):2115–2121CrossRefPubMed
28.
go back to reference Erickson R (1992) Surface interactions of dental adhesive materials. Oper Dent 5:81–94PubMed Erickson R (1992) Surface interactions of dental adhesive materials. Oper Dent 5:81–94PubMed
29.
go back to reference Versiani MA, Carvalho-Junior JR, Padilha MIAF, Lacey S, Pascon EA, Sousa-Neto MD (2006) A comparative study of physicochemical properties of AH Plus and Epiphany root canal sealants. Int Endod J 39:464–471CrossRefPubMed Versiani MA, Carvalho-Junior JR, Padilha MIAF, Lacey S, Pascon EA, Sousa-Neto MD (2006) A comparative study of physicochemical properties of AH Plus and Epiphany root canal sealants. Int Endod J 39:464–471CrossRefPubMed
30.
go back to reference Tsui-Hsien H, Chong-Kuei L, Ming-Yung C, Chia-Tze K (2000) Lactate dehydrogenase leakage of hepatocytes with AH26 and AH Plus sealer treatments JOE, VOL. 26, NO. 9, SEPTEMBER 2000 Tsui-Hsien H, Chong-Kuei L, Ming-Yung C, Chia-Tze K (2000) Lactate dehydrogenase leakage of hepatocytes with AH26 and AH Plus sealer treatments JOE, VOL. 26, NO. 9, SEPTEMBER 2000
31.
go back to reference Ngoh EC, Pashley DH, Loushine RJ, Weller RN, Kimbrough WF (2001) Effects of eugenol on resin bond strengths to root canal dentin. J Endod 27:411–414CrossRefPubMed Ngoh EC, Pashley DH, Loushine RJ, Weller RN, Kimbrough WF (2001) Effects of eugenol on resin bond strengths to root canal dentin. J Endod 27:411–414CrossRefPubMed
32.
go back to reference Schwartz RS (2006) Adhesive dentistry and endodontics (2006) bonding in the root canal system-the promise and the problems: a review. J Endod 32(12):1125–1134CrossRefPubMed Schwartz RS (2006) Adhesive dentistry and endodontics (2006) bonding in the root canal system-the promise and the problems: a review. J Endod 32(12):1125–1134CrossRefPubMed
33.
go back to reference Onay EO, Orucoglu H, Kiremitci A, Korkmaz Y, Berk G (2010) Effect of Er,Cr:YSGG laser irradiation on the apical sealing ability of AH Plus/gutta-percha and hybrid root seal/Resilon combinations. Oral Surg Oral Med Oral Pathol Oral Radiol 110:657–664CrossRef Onay EO, Orucoglu H, Kiremitci A, Korkmaz Y, Berk G (2010) Effect of Er,Cr:YSGG laser irradiation on the apical sealing ability of AH Plus/gutta-percha and hybrid root seal/Resilon combinations. Oral Surg Oral Med Oral Pathol Oral Radiol 110:657–664CrossRef
34.
go back to reference Uzunoglu E, Aktemur S, Uyanik MO, Durmaz V, Nagas E (2012) Effect of ethylenediaminetetraacetic acid on root fracture with respect to concentration at different time exposures. J Endod 38:1110–1113CrossRefPubMed Uzunoglu E, Aktemur S, Uyanik MO, Durmaz V, Nagas E (2012) Effect of ethylenediaminetetraacetic acid on root fracture with respect to concentration at different time exposures. J Endod 38:1110–1113CrossRefPubMed
35.
36.
go back to reference Martins M, Franzen R, Depraet F, Gutknecht N (2018) Rationale for using a double-wavelength (940 nm + 2780 nm) laser in endodontics: literature overview and proof-of-concept. Lasers Dent Sci 2:29–41CrossRef Martins M, Franzen R, Depraet F, Gutknecht N (2018) Rationale for using a double-wavelength (940 nm + 2780 nm) laser in endodontics: literature overview and proof-of-concept. Lasers Dent Sci 2:29–41CrossRef
37.
go back to reference Goldman M, Goldman LB, Cavaleri R, Bogis J, Lin PS (1982) The efficacy of several endodontic irrigating solutions: a scanning electron microscopic study: part 2. J Endod 8:487–492CrossRefPubMed Goldman M, Goldman LB, Cavaleri R, Bogis J, Lin PS (1982) The efficacy of several endodontic irrigating solutions: a scanning electron microscopic study: part 2. J Endod 8:487–492CrossRefPubMed
38.
go back to reference Chen WP, Chen YY, Huang SH, Lin CP (2013) Limitations of push-out test in bond strength measurement. J Endod 39(2):283–287CrossRefPubMed Chen WP, Chen YY, Huang SH, Lin CP (2013) Limitations of push-out test in bond strength measurement. J Endod 39(2):283–287CrossRefPubMed
39.
go back to reference Pane ES, Palamara JE, Messer HH (2013) Critical evaluation of the push-out test for root canal filling materials. J Endod 39(5):669–673CrossRefPubMed Pane ES, Palamara JE, Messer HH (2013) Critical evaluation of the push-out test for root canal filling materials. J Endod 39(5):669–673CrossRefPubMed
40.
go back to reference Jainaen A, Palamara JE, Messer HH (2007) Push-out bond strengths of the dentine-sealer interface with and without a main cone. Int Endod J 40:882–890CrossRefPubMed Jainaen A, Palamara JE, Messer HH (2007) Push-out bond strengths of the dentine-sealer interface with and without a main cone. Int Endod J 40:882–890CrossRefPubMed
41.
go back to reference Nagas E, Uyanik O, Durmaz V, Cehreli ZC (2011) Effect of plunger diameter on the push-out bond values of different root filling materials. Int Endod J 44(10):950–955CrossRefPubMed Nagas E, Uyanik O, Durmaz V, Cehreli ZC (2011) Effect of plunger diameter on the push-out bond values of different root filling materials. Int Endod J 44(10):950–955CrossRefPubMed
42.
go back to reference Haragushiku GA, Sousa-Neto MD, Silva-Sousa YT, Alfredo E, Silva SC, Silva RG (2010) Adhesion of endodontic sealers to human root dentine submitted to different surface treatments. Photomed Laser Surg 28(3):405–410CrossRefPubMed Haragushiku GA, Sousa-Neto MD, Silva-Sousa YT, Alfredo E, Silva SC, Silva RG (2010) Adhesion of endodontic sealers to human root dentine submitted to different surface treatments. Photomed Laser Surg 28(3):405–410CrossRefPubMed
43.
go back to reference Lee KW, Williams MC, Camps JJ, Pashley DH (2002) Adhesion of endodontic sealers to dentin and gutta-percha. J Endod 28:684–688CrossRefPubMed Lee KW, Williams MC, Camps JJ, Pashley DH (2002) Adhesion of endodontic sealers to dentin and gutta-percha. J Endod 28:684–688CrossRefPubMed
44.
go back to reference Fisher MA, Berzins DW, Bahcall JK (2007) An in vitro comparison of bond strength of various obturation materials to root canal dentin using a push-out test design. J Endod 33:856–858CrossRefPubMed Fisher MA, Berzins DW, Bahcall JK (2007) An in vitro comparison of bond strength of various obturation materials to root canal dentin using a push-out test design. J Endod 33:856–858CrossRefPubMed
45.
go back to reference Moura-Net to CPT, Davidowicz H, de Moura AA (2009) Apical leakage of three resin-based endodontic sealers after 810-nm diode laser irradiation. Photomed Laser Surg 27(6):891–894CrossRef Moura-Net to CPT, Davidowicz H, de Moura AA (2009) Apical leakage of three resin-based endodontic sealers after 810-nm diode laser irradiation. Photomed Laser Surg 27(6):891–894CrossRef
Metadata
Title
In vitro evaluation of the push-out bond strength of the epoxy resin-based root canal sealer to root dentin irradiated with double-wavelength lasers
Authors
Massoud Rahati
Ezatolah Kazeminejad
Sareh Said Yekta-Michael
Norbert Gutknecht
Publication date
01-09-2019
Publisher
Springer International Publishing
Published in
Lasers in Dental Science / Issue 3/2019
Electronic ISSN: 2367-2587
DOI
https://doi.org/10.1007/s41547-019-00067-6

Other articles of this Issue 3/2019

Lasers in Dental Science 3/2019 Go to the issue