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BMC Medical Imaging
Published in: BMC Medical Imaging 1/2020

Open Access 01-12-2020 | Digital Volume Tomography | Research article

Sentinel lymph node mapping using ICG fluorescence and cone beam CT – a feasibility study in a rabbit model of oral cancer

Authors: Nidal Muhanna, Harley H. L. Chan, Catriona M. Douglas, Michael J. Daly, Atul Jaidka, Donovan Eu, Jonathan Bernstein, Jason L. Townson, Jonathan C. Irish

Published in: BMC Medical Imaging | Issue 1/2020

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Abstract

Background

Current sentinel lymph node biopsy (SLNB) techniques, including use of radioisotopes, have disadvantages including the use of a radioactive tracer. Indocyanine green (ICG) based near-infrared (NIR) fluorescence imaging and cone beam CT (CBCT) have advantages for intraoperative use. However, limited literature exists regarding their use in head and neck cancer SLNB.

Methods

This was a prospective, non-randomized study using a rabbit oral cavity VX2 squamous cell carcinoma model (n = 10) which develops lymph node metastasis. Pre-operatively, images were acquired by MicroCT. During surgery, CBCT and NIR fluorescence imaging of ICG was used to map and guide the SLNB resection.

Results

Intraoperative use of ICG to guide fluorescence resection resulted in identification of all lymph nodes identified by pre-operative CT. CBCT was useful for near real time intraoperative imaging and 3D reconstruction.

Conclusions

This pre-clinical study further demonstrates the technical feasibility, limitations and advantages of intraoperative NIR-guided ICG imaging for SLN identification as a complementary method during head and neck surgery.
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Metadata
Title
Sentinel lymph node mapping using ICG fluorescence and cone beam CT – a feasibility study in a rabbit model of oral cancer
Authors
Nidal Muhanna
Harley H. L. Chan
Catriona M. Douglas
Michael J. Daly
Atul Jaidka
Donovan Eu
Jonathan Bernstein
Jason L. Townson
Jonathan C. Irish
Publication date
01-12-2020
Publisher
BioMed Central
Published in
BMC Medical Imaging / Issue 1/2020
Electronic ISSN: 1471-2342
DOI
https://doi.org/10.1186/s12880-020-00507-x

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